Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy
Acute schistosomiasis is associated with a primary exposure and is more commonly seen in nonimmune individuals traveling through endemic regions. In this study, we have focused on the cytokine profile of T lymphocytes evaluated in circulating leukocytes of acute Schistosomiasis mansoni-infected pati...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2013/909134 |
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| author | Denise Silveira-Lemos Matheus Fernandes Costa-Silva Amanda Cardoso de Oliveira Silveira Mauricio Azevedo Batista Lúcia Alves Oliveira-Fraga Alda Maria Soares Silveira Maria Carolina Barbosa Alvarez Olindo Assis Martins-Filho Giovanni Gazzinelli Rodrigo Corrêa-Oliveira Andréa Teixeira-Carvalho |
| author_facet | Denise Silveira-Lemos Matheus Fernandes Costa-Silva Amanda Cardoso de Oliveira Silveira Mauricio Azevedo Batista Lúcia Alves Oliveira-Fraga Alda Maria Soares Silveira Maria Carolina Barbosa Alvarez Olindo Assis Martins-Filho Giovanni Gazzinelli Rodrigo Corrêa-Oliveira Andréa Teixeira-Carvalho |
| author_sort | Denise Silveira-Lemos |
| collection | DOAJ |
| description | Acute schistosomiasis is associated with a primary exposure and is more commonly seen in nonimmune individuals traveling through endemic regions. In this study, we have focused on the cytokine profile of T lymphocytes evaluated in circulating leukocytes of acute Schistosomiasis mansoni-infected patients (ACT group) before and after praziquantel treatment (ACT-TR group). Our data demonstrated increased values of total leukocytes, eosinophils, and monocytes in both groups. Interestingly, we have observed that patients treated with praziquantel showed increased values of lymphocytes as compared with noninfected group (NI) or ACT groups. Furthermore, a decrease of neutrophils in ACT-TR was observed when compared to ACT group. Analyses of short-term in vitro whole blood stimulation demonstrated that, regardless of the presence of soluble Schistosoma mansoni eggs antigen (SEA), increased synthesis of IFN-γ and IL-4 by T-cells was observed in the ACT group. Analyses of cytokine profile in CD8 T cells demonstrated higher percentage of IFN-γ and IL-4 cells in both ACT and ACT-TR groups apart from increased percentage of IL-10 cells only in the ACT group. This study is the first one to point out the relevance of CD8 T lymphocytes in the immune response induced during the acute phase of schistosomiasis. |
| format | Article |
| id | doaj-art-c85c9fb451d0467bb4c772f0f75d8039 |
| institution | DOAJ |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-c85c9fb451d0467bb4c772f0f75d80392025-08-20T03:24:02ZengWileyJournal of Parasitology Research2090-00232090-00312013-01-01201310.1155/2013/909134909134Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel TherapyDenise Silveira-Lemos0Matheus Fernandes Costa-Silva1Amanda Cardoso de Oliveira Silveira2Mauricio Azevedo Batista3Lúcia Alves Oliveira-Fraga4Alda Maria Soares Silveira5Maria Carolina Barbosa Alvarez6Olindo Assis Martins-Filho7Giovanni Gazzinelli8Rodrigo Corrêa-Oliveira9Andréa Teixeira-Carvalho10Laboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, FIOCRUZ, Barro Preto, 30190-002 Belo Horizonte, MG, BrazilLaboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, FIOCRUZ, Barro Preto, 30190-002 Belo Horizonte, MG, BrazilLaboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, FIOCRUZ, Barro Preto, 30190-002 Belo Horizonte, MG, BrazilLaboratório de Imunoparasitologia, Departamento de Ciências Biológicas, NUPEB, Instituto de Ciências Exatas e Biológicas, Universidade Federal de Ouro Preto, Ouro Preto, MG, BrazilNúcleo de Pesquisa em Imunologia, Faculdade de Ciências da Saúde, Universidade Vale do Rio Doce, Governador Valadares, MG, BrazilNúcleo de Pesquisa em Imunologia, Faculdade de Ciências da Saúde, Universidade Vale do Rio Doce, Governador Valadares, MG, BrazilSanta Casa de Misericórdia de Belo Horizonte, Belo Horizonte, MG, BrazilLaboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, FIOCRUZ, Barro Preto, 30190-002 Belo Horizonte, MG, BrazilLaboratório de Imunologia Celular e Molecular, Centro de Pesquisas René Rachou, FIOCRUZ, 30190-002 Belo Horizonte, MG, BrazilLaboratório de Imunologia Celular e Molecular, Centro de Pesquisas René Rachou, FIOCRUZ, 30190-002 Belo Horizonte, MG, BrazilLaboratório de Biomarcadores de Diagnóstico e Monitoração, Centro de Pesquisas René Rachou, FIOCRUZ, Barro Preto, 30190-002 Belo Horizonte, MG, BrazilAcute schistosomiasis is associated with a primary exposure and is more commonly seen in nonimmune individuals traveling through endemic regions. In this study, we have focused on the cytokine profile of T lymphocytes evaluated in circulating leukocytes of acute Schistosomiasis mansoni-infected patients (ACT group) before and after praziquantel treatment (ACT-TR group). Our data demonstrated increased values of total leukocytes, eosinophils, and monocytes in both groups. Interestingly, we have observed that patients treated with praziquantel showed increased values of lymphocytes as compared with noninfected group (NI) or ACT groups. Furthermore, a decrease of neutrophils in ACT-TR was observed when compared to ACT group. Analyses of short-term in vitro whole blood stimulation demonstrated that, regardless of the presence of soluble Schistosoma mansoni eggs antigen (SEA), increased synthesis of IFN-γ and IL-4 by T-cells was observed in the ACT group. Analyses of cytokine profile in CD8 T cells demonstrated higher percentage of IFN-γ and IL-4 cells in both ACT and ACT-TR groups apart from increased percentage of IL-10 cells only in the ACT group. This study is the first one to point out the relevance of CD8 T lymphocytes in the immune response induced during the acute phase of schistosomiasis.http://dx.doi.org/10.1155/2013/909134 |
| spellingShingle | Denise Silveira-Lemos Matheus Fernandes Costa-Silva Amanda Cardoso de Oliveira Silveira Mauricio Azevedo Batista Lúcia Alves Oliveira-Fraga Alda Maria Soares Silveira Maria Carolina Barbosa Alvarez Olindo Assis Martins-Filho Giovanni Gazzinelli Rodrigo Corrêa-Oliveira Andréa Teixeira-Carvalho Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy Journal of Parasitology Research |
| title | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
| title_full | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
| title_fullStr | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
| title_full_unstemmed | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
| title_short | Cytokine Pattern of T Lymphocytes in Acute Schistosomiasis mansoni Patients following Treated Praziquantel Therapy |
| title_sort | cytokine pattern of t lymphocytes in acute schistosomiasis mansoni patients following treated praziquantel therapy |
| url | http://dx.doi.org/10.1155/2013/909134 |
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