A convergent fabrication of programmed pH/reduction-responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatment
A nanoparticle-based drug delivery technology could develop combination cancer therapy more effectively. However, because of inadequate drug delivery into tumor cells, the cancer therapeutic efficiency of nanomedicines is diminished. PEGylated poly(α-lipoic acid) copolymers with (mPEG-PLA) were fabr...
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| Format: | Article |
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Taylor & Francis Group
2023-12-01
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| Series: | Journal of Experimental Nanoscience |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/17458080.2023.2193400 |
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| author | Haiyan Zhang Youlin Yang Yi Chen Xiahui Zhang Xiaopei Chen |
| author_facet | Haiyan Zhang Youlin Yang Yi Chen Xiahui Zhang Xiaopei Chen |
| author_sort | Haiyan Zhang |
| collection | DOAJ |
| description | A nanoparticle-based drug delivery technology could develop combination cancer therapy more effectively. However, because of inadequate drug delivery into tumor cells, the cancer therapeutic efficiency of nanomedicines is diminished. PEGylated poly(α-lipoic acid) copolymers with (mPEG-PLA) were fabricated and used as pH/reductive responsive nanovesicles to administer Gefitinib (GFT) and doxorubicin (DOX) for the treatment of ovarian cancer. The amphiphilic polymers mPEG-PLA may be efficiently incorporated on DOX and GFT to fabricate DOX and GFT coloaded nanoparticles (DOX@GFT-NPs) and self-assembled into an aqueous solution. The DOX@GFT-NPs released more DOX and GFT after being prepared to respond to pH and reduction stimuli. The outcomes of confocal laser scanning microscopy and flow cytometry findings, the SKOV3 ovarian cancer cells quickly fascinated the dual drugs-coloaded nanoparticles and drugs released intracellularly accumulated. DOX@GFT-NPs triggered cell death and demonstrated synergistic therapeutic benefits in SKOV3 cells. Results showed that the nanoparticles efficiently trigger apoptosis in SKOV3 ovarian cancer cells using morphological staining (acridine orange/ethidium bromide (AO/EB) and HOECHST 33342 nuclear staining). These outcomes show that using pH and reduction stimuli on mPEG-PLA copolymer to treat ovarian cancer is a promising approach. |
| format | Article |
| id | doaj-art-c85bc74cfa254b8a80c829cc94c95dc1 |
| institution | OA Journals |
| issn | 1745-8080 1745-8099 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Experimental Nanoscience |
| spelling | doaj-art-c85bc74cfa254b8a80c829cc94c95dc12025-08-20T01:53:33ZengTaylor & Francis GroupJournal of Experimental Nanoscience1745-80801745-80992023-12-0118110.1080/17458080.2023.2193400A convergent fabrication of programmed pH/reduction-responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatmentHaiyan Zhang0Youlin Yang1Yi Chen2Xiahui Zhang3Xiaopei Chen4Department of Obstetrics and Gynecology, The First People’s Hospital of Wenling, Wenling, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Wenling, Wenling, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Wenling, Wenling, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Wenling, Wenling, ChinaDepartment of Obstetrics and Gynecology, The First People’s Hospital of Wenling, Wenling, ChinaA nanoparticle-based drug delivery technology could develop combination cancer therapy more effectively. However, because of inadequate drug delivery into tumor cells, the cancer therapeutic efficiency of nanomedicines is diminished. PEGylated poly(α-lipoic acid) copolymers with (mPEG-PLA) were fabricated and used as pH/reductive responsive nanovesicles to administer Gefitinib (GFT) and doxorubicin (DOX) for the treatment of ovarian cancer. The amphiphilic polymers mPEG-PLA may be efficiently incorporated on DOX and GFT to fabricate DOX and GFT coloaded nanoparticles (DOX@GFT-NPs) and self-assembled into an aqueous solution. The DOX@GFT-NPs released more DOX and GFT after being prepared to respond to pH and reduction stimuli. The outcomes of confocal laser scanning microscopy and flow cytometry findings, the SKOV3 ovarian cancer cells quickly fascinated the dual drugs-coloaded nanoparticles and drugs released intracellularly accumulated. DOX@GFT-NPs triggered cell death and demonstrated synergistic therapeutic benefits in SKOV3 cells. Results showed that the nanoparticles efficiently trigger apoptosis in SKOV3 ovarian cancer cells using morphological staining (acridine orange/ethidium bromide (AO/EB) and HOECHST 33342 nuclear staining). These outcomes show that using pH and reduction stimuli on mPEG-PLA copolymer to treat ovarian cancer is a promising approach.https://www.tandfonline.com/doi/10.1080/17458080.2023.2193400Anticancer drugsapoptosisovarian cancerco-polymerdrugs release |
| spellingShingle | Haiyan Zhang Youlin Yang Yi Chen Xiahui Zhang Xiaopei Chen A convergent fabrication of programmed pH/reduction-responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatment Journal of Experimental Nanoscience Anticancer drugs apoptosis ovarian cancer co-polymer drugs release |
| title | A convergent fabrication of programmed pH/reduction-responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatment |
| title_full | A convergent fabrication of programmed pH/reduction-responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatment |
| title_fullStr | A convergent fabrication of programmed pH/reduction-responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatment |
| title_full_unstemmed | A convergent fabrication of programmed pH/reduction-responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatment |
| title_short | A convergent fabrication of programmed pH/reduction-responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatment |
| title_sort | convergent fabrication of programmed ph reduction responsive nanoparticles for efficient dual anticancer drugs delivery for ovarian cancer treatment |
| topic | Anticancer drugs apoptosis ovarian cancer co-polymer drugs release |
| url | https://www.tandfonline.com/doi/10.1080/17458080.2023.2193400 |
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