Aptamer and Oligonucleotide-Based Biosensors for Health Applications
Aptamers have emerged as powerful molecular recognition elements for biosensing applications, offering high specificity, stability, and adaptability. This review explores key considerations in designing aptamer-based sensors (aptasensors), with a focus on biomarker selection, aptamer design, and det...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-04-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/5/277 |
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| author | Beatriz Mayol I. Zeina Qubbaj Julieta Nava-Granados Katherine Vasquez Scott T. Keene Juliane R. Sempionatto |
| author_facet | Beatriz Mayol I. Zeina Qubbaj Julieta Nava-Granados Katherine Vasquez Scott T. Keene Juliane R. Sempionatto |
| author_sort | Beatriz Mayol |
| collection | DOAJ |
| description | Aptamers have emerged as powerful molecular recognition elements for biosensing applications, offering high specificity, stability, and adaptability. This review explores key considerations in designing aptamer-based sensors (aptasensors), with a focus on biomarker selection, aptamer design, and detection and immobilization strategies. However, challenges such as biofluid stability and reversibility must be addressed to improve biosensor performance. In this study, the potential of aptamer-based platforms in diagnostics is explored, emphasizing their advantages and future applications. Looking ahead, advances in multifunctional aptamers, integration with nanomaterials, and computational optimization are highlighted as promising directions for enhancing their effectiveness in biosensing. |
| format | Article |
| id | doaj-art-c8560b0c8a834398abc459dbec0d31a2 |
| institution | OA Journals |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-c8560b0c8a834398abc459dbec0d31a22025-08-20T01:56:20ZengMDPI AGBiosensors2079-63742025-04-0115527710.3390/bios15050277Aptamer and Oligonucleotide-Based Biosensors for Health ApplicationsBeatriz Mayol0I. Zeina Qubbaj1Julieta Nava-Granados2Katherine Vasquez3Scott T. Keene4Juliane R. Sempionatto5Department of Electrical and Computer Engineering, Rice University, Houston, TX 77005, USADepartment of Materials Science and NanoEngineering, Rice University, Houston, TX 77005, USADepartment of Electrical and Computer Engineering, Rice University, Houston, TX 77005, USADepartment of Electrical and Computer Engineering, Rice University, Houston, TX 77005, USADepartment of Materials Science and NanoEngineering, Rice University, Houston, TX 77005, USADepartment of Electrical and Computer Engineering, Rice University, Houston, TX 77005, USAAptamers have emerged as powerful molecular recognition elements for biosensing applications, offering high specificity, stability, and adaptability. This review explores key considerations in designing aptamer-based sensors (aptasensors), with a focus on biomarker selection, aptamer design, and detection and immobilization strategies. However, challenges such as biofluid stability and reversibility must be addressed to improve biosensor performance. In this study, the potential of aptamer-based platforms in diagnostics is explored, emphasizing their advantages and future applications. Looking ahead, advances in multifunctional aptamers, integration with nanomaterials, and computational optimization are highlighted as promising directions for enhancing their effectiveness in biosensing.https://www.mdpi.com/2079-6374/15/5/277aptamer biosensorelectrochemical aptasensorhealth monitoring |
| spellingShingle | Beatriz Mayol I. Zeina Qubbaj Julieta Nava-Granados Katherine Vasquez Scott T. Keene Juliane R. Sempionatto Aptamer and Oligonucleotide-Based Biosensors for Health Applications Biosensors aptamer biosensor electrochemical aptasensor health monitoring |
| title | Aptamer and Oligonucleotide-Based Biosensors for Health Applications |
| title_full | Aptamer and Oligonucleotide-Based Biosensors for Health Applications |
| title_fullStr | Aptamer and Oligonucleotide-Based Biosensors for Health Applications |
| title_full_unstemmed | Aptamer and Oligonucleotide-Based Biosensors for Health Applications |
| title_short | Aptamer and Oligonucleotide-Based Biosensors for Health Applications |
| title_sort | aptamer and oligonucleotide based biosensors for health applications |
| topic | aptamer biosensor electrochemical aptasensor health monitoring |
| url | https://www.mdpi.com/2079-6374/15/5/277 |
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