Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective
IntroductionClinical metritis (CM) has significant costs to dairy producers. Current treatment strategy involves systemic antibiotics; however, there is increasing concern about judicious antibiotic use. The study objective was to evaluate the effects of a non-antibiotic treatment vs. systemic antib...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-12-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1478288/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849727999864209408 |
|---|---|
| author | Jennine Lection Jennine Lection Jennine Lection Emily Van Syoc Emily Van Syoc Emily Van Syoc Asha Miles Asha Miles Julia Hamilton Marcela Martinez Santiago Bas Justin Silverman Justin Silverman Justin Silverman Adrian Barragan Erika Ganda Erika Ganda Erika Ganda |
| author_facet | Jennine Lection Jennine Lection Jennine Lection Emily Van Syoc Emily Van Syoc Emily Van Syoc Asha Miles Asha Miles Julia Hamilton Marcela Martinez Santiago Bas Justin Silverman Justin Silverman Justin Silverman Adrian Barragan Erika Ganda Erika Ganda Erika Ganda |
| author_sort | Jennine Lection |
| collection | DOAJ |
| description | IntroductionClinical metritis (CM) has significant costs to dairy producers. Current treatment strategy involves systemic antibiotics; however, there is increasing concern about judicious antibiotic use. The study objective was to evaluate the effects of a non-antibiotic treatment vs. systemic antibiotic therapy on the vaginal discharge microbiome of dairy cows diagnosed with CM at 7 ± 3 DIM (days in milk). We hypothesize that both treatment methods will have a similar impact on the reproductive microbiome due to broad-spectrum bactericidal activity; therefore, there will not be significant differences amongst the microbiota after the completion of therapy.MethodsCows from a central Pennsylvania dairy were screened for CM at 7 ± 3 days DIM using a Metricheck™ device (n = 351). Cows with red-brown watery discharge were diagnosed with CM and eligible for enrollment. Eligible cows (n = 77) were blocked by parity and randomly allocated to one of two treatments starting on the day of diagnosis: (1) Intrauterine dextrose (DEX, n = 38): 1 l of an intrauterine 50% dextrose solution for 3 days, and (2) Systemic ceftiofur (CONV, n = 39): two injections of ceftiofur (6.6 mg/Kg of BW; Excede, Zoetis Inc.) 72 h apart. Cows were evaluated for clinical cure rate at 7 ± 3 and 14 ± 3 days post-diagnosis. Vaginal discharge samples were collected using the Metricheck™ at enrollment day [study day (sd) 0, pre-treatment], sd 7, and sd 14 for a subset of enrolled cows (DEX = 13, CONV = 14). Vaginal discharge samples were analyzed with 16S rRNA sequencing to evaluate changes in the microbiome between treatments.ResultsAfter treatment, there were only minor differences within the microbiome between the two treatments indicating the potential suitability of dextrose as an antibiotic-alternative treatment. Alpha diversity did not differ (Welch's t-test) between the treatments at any of the time points. Beta diversity based on PERMANOVA analysis did differ between treatments at sd 0 (P = 0.014) and again at sd 14 (P = 0.028), but not at sd 7 (P = 0.261).DiscussionWhile 16S rRNA analysis does not provide information on bacterial viability, the relative similarity of the microbiome between the two groups immediately following treatment might suggest that intrauterine dextrose could be utilized as an alternative treatment for CM. |
| format | Article |
| id | doaj-art-c852b27ff2b34099ac690b875c5dfdaa |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-c852b27ff2b34099ac690b875c5dfdaa2025-08-20T03:09:42ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.14782881478288Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspectiveJennine Lection0Jennine Lection1Jennine Lection2Emily Van Syoc3Emily Van Syoc4Emily Van Syoc5Asha Miles6Asha Miles7Julia Hamilton8Marcela Martinez9Santiago Bas10Justin Silverman11Justin Silverman12Justin Silverman13Adrian Barragan14Erika Ganda15Erika Ganda16Erika Ganda17Intergraduate Degree Program in Integrative and Biomedical Physiology, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, United StatesDepartment of Animal Science, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, United StatesOne Health Microbiome Center, The Pennsylvania State University, University Park, PA, United StatesDepartment of Animal Science, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, United StatesOne Health Microbiome Center, The Pennsylvania State University, University Park, PA, United StatesDepartment of Biology, Eberly College of Science, The Pennsylvania State University, University Park, PA, United StatesDepartment of Animal Science, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, United StatesAnimal Genomics and Improvement Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD, United StatesDepartment of Veterinary and Biomedical Sciences, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, United StatesDepartment of Veterinary and Biomedical Sciences, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, United StatesPhytobiotics Futterzusatzstoffe GmbH Bvd Villa Maria Córdoba Argentina, Villa Maria, ArgentinaCollege of Information Sciences and Technology, The Pennsylvania State University, University Park, PA, United StatesDepartment of Medicine, The Pennsylvania State University, Hershey, PA, United States0Institute for Computational and Data Science, The Pennsylvania State University, University Park, PA, United StatesDepartment of Veterinary and Biomedical Sciences, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, United StatesIntergraduate Degree Program in Integrative and Biomedical Physiology, Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, United StatesDepartment of Animal Science, College of Agricultural Sciences, The Pennsylvania State University, University Park, PA, United StatesOne Health Microbiome Center, The Pennsylvania State University, University Park, PA, United StatesIntroductionClinical metritis (CM) has significant costs to dairy producers. Current treatment strategy involves systemic antibiotics; however, there is increasing concern about judicious antibiotic use. The study objective was to evaluate the effects of a non-antibiotic treatment vs. systemic antibiotic therapy on the vaginal discharge microbiome of dairy cows diagnosed with CM at 7 ± 3 DIM (days in milk). We hypothesize that both treatment methods will have a similar impact on the reproductive microbiome due to broad-spectrum bactericidal activity; therefore, there will not be significant differences amongst the microbiota after the completion of therapy.MethodsCows from a central Pennsylvania dairy were screened for CM at 7 ± 3 days DIM using a Metricheck™ device (n = 351). Cows with red-brown watery discharge were diagnosed with CM and eligible for enrollment. Eligible cows (n = 77) were blocked by parity and randomly allocated to one of two treatments starting on the day of diagnosis: (1) Intrauterine dextrose (DEX, n = 38): 1 l of an intrauterine 50% dextrose solution for 3 days, and (2) Systemic ceftiofur (CONV, n = 39): two injections of ceftiofur (6.6 mg/Kg of BW; Excede, Zoetis Inc.) 72 h apart. Cows were evaluated for clinical cure rate at 7 ± 3 and 14 ± 3 days post-diagnosis. Vaginal discharge samples were collected using the Metricheck™ at enrollment day [study day (sd) 0, pre-treatment], sd 7, and sd 14 for a subset of enrolled cows (DEX = 13, CONV = 14). Vaginal discharge samples were analyzed with 16S rRNA sequencing to evaluate changes in the microbiome between treatments.ResultsAfter treatment, there were only minor differences within the microbiome between the two treatments indicating the potential suitability of dextrose as an antibiotic-alternative treatment. Alpha diversity did not differ (Welch's t-test) between the treatments at any of the time points. Beta diversity based on PERMANOVA analysis did differ between treatments at sd 0 (P = 0.014) and again at sd 14 (P = 0.028), but not at sd 7 (P = 0.261).DiscussionWhile 16S rRNA analysis does not provide information on bacterial viability, the relative similarity of the microbiome between the two groups immediately following treatment might suggest that intrauterine dextrose could be utilized as an alternative treatment for CM.https://www.frontiersin.org/articles/10.3389/fvets.2024.1478288/fullantibiotic alternativeclinical metritisdairy cattleintrauterine dextrosemicrobiome |
| spellingShingle | Jennine Lection Jennine Lection Jennine Lection Emily Van Syoc Emily Van Syoc Emily Van Syoc Asha Miles Asha Miles Julia Hamilton Marcela Martinez Santiago Bas Justin Silverman Justin Silverman Justin Silverman Adrian Barragan Erika Ganda Erika Ganda Erika Ganda Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective Frontiers in Veterinary Science antibiotic alternative clinical metritis dairy cattle intrauterine dextrose microbiome |
| title | Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective |
| title_full | Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective |
| title_fullStr | Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective |
| title_full_unstemmed | Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective |
| title_short | Use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle: a microbiome perspective |
| title_sort | use of intrauterine dextrose as an alternative to systemic antibiotics for treatment of clinical metritis in dairy cattle a microbiome perspective |
| topic | antibiotic alternative clinical metritis dairy cattle intrauterine dextrose microbiome |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1478288/full |
| work_keys_str_mv | AT jenninelection useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT jenninelection useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT jenninelection useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT emilyvansyoc useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT emilyvansyoc useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT emilyvansyoc useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT ashamiles useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT ashamiles useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT juliahamilton useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT marcelamartinez useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT santiagobas useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT justinsilverman useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT justinsilverman useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT justinsilverman useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT adrianbarragan useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT erikaganda useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT erikaganda useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective AT erikaganda useofintrauterinedextroseasanalternativetosystemicantibioticsfortreatmentofclinicalmetritisindairycattleamicrobiomeperspective |