Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with Effusion
Zhihe Lin,1,* Chuntao Deng,2,* Dan Wang,3,* Bo Lyu,1 Jiaying Pan,4 Jinlian Li,1 Minting Chen,1 Zhaoxia Zhang,5 Zibin Liang,6 Lei Chen,7 Shaoyan Feng1 1Department of Otolaryngology, Head and Neck Surgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhu...
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Dove Medical Press
2025-05-01
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| Series: | Journal of Inflammation Research |
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| Online Access: | https://www.dovepress.com/expression-and-clinical-significance-of-il-33-and-il-25-in-post-irradi-peer-reviewed-fulltext-article-JIR |
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| author | Lin Z Deng C Wang D Lyu B Pan J Li J Chen M Zhang Z Liang Z Chen L Feng S |
| author_facet | Lin Z Deng C Wang D Lyu B Pan J Li J Chen M Zhang Z Liang Z Chen L Feng S |
| author_sort | Lin Z |
| collection | DOAJ |
| description | Zhihe Lin,1,&ast; Chuntao Deng,2,&ast; Dan Wang,3,&ast; Bo Lyu,1 Jiaying Pan,4 Jinlian Li,1 Minting Chen,1 Zhaoxia Zhang,5 Zibin Liang,6 Lei Chen,7 Shaoyan Feng1 1Department of Otolaryngology, Head and Neck Surgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 2Department of Radiation Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 3Department of Gastrointestinal Surgery, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 4Department of Otolaryngology, Eye and ENT Hospital, ENT Institute, Fudan University, Zhuhai, Guangdong, People’s Republic of China; 5Office of Organ Transplant Management, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 6Department of Thoracic Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 7Department of Neurosurgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Shaoyan Feng, Email fengshaoyan97@163.com; fshaoy@mail.sysu.edu.cn Lei Chen, Email clzzzz@126.comPurpose: This study investigates IL-33 and IL-25 expression in post-radiation otitis media with effusion (POME), along with classic oxidative stress markers (MDA and SOD), to investigate radiation-induced oxidative stress and its association with ILC2-mediated chronic inflammation, providing basis for targeted therapies.Methods: Middle ear effusions (MEE) were collected from 35 irradiated nasopharyngeal carcinoma patients with otitis media with effusion (POME, 43 ears) and 20 non-irradiated conventional chronic otitis media with effusion (CCOME, 20 ears) patients. IL-33, IL-25, IL-6, SOD, and MDA levels were measured by ELISA. Eustachian tube function was evaluated using the Endoscopic Evaluation of the Eustachian Tube (3ET) scoring system.Results: Comparative analysis revealed distinct molecular profiles between POME and CCOME, with POME showing significantly reduced IL-33 levels (p=0.046) but elevated SOD activity (p=0.015), along with a non-significant trend toward higher MDA (p=0.083). Temporal analysis demonstrated peak expression of both IL-25 and IL-33 at 6 months post-radiation. Correlation studies identified significant associations between IL-33 (r=0.391) and IL-6, as well as between IL-25 (r=0.483) and IL-6 (both p< 0.01). Clinically, IL-25 levels showed positive correlation with 3ET endoscopic scores (r=0.407, p=0.021) and were significantly reduced following tympanostomy tube placement (p=0.024). Notably, no direct correlation was observed between IL-33 and IL-25 (p> 0.05), nor were any significant associations found with allergic comorbidities (all p> 0.05).Conclusion: IL-33 and IL-25 synergistically drive ILC2-mediated chronic inflammation in radiation-induced OME, with IL-25 emerging as a biomarker for radiotherapy-associated Eustachian tube dysfunction. The 6-month cytokine surge post-radiation highlights a therapeutic window for targeted interventions to mitigate long-term complications. Keywords: post-irradiation, otitis media with effusion, nasopharyngeal carcinoma, interleukin-33, interleukin-25 |
| format | Article |
| id | doaj-art-c84cf9436dd84a7ab294cfccb297cf03 |
| institution | DOAJ |
| issn | 1178-7031 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Inflammation Research |
| spelling | doaj-art-c84cf9436dd84a7ab294cfccb297cf032025-08-20T03:24:00ZengDove Medical PressJournal of Inflammation Research1178-70312025-05-01Volume 18Issue 168776887103347Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with EffusionLin Z0Deng C1Wang D2Lyu B3Pan J4Li J5Chen M6Zhang Z7Liang Z8Chen L9Feng S10Department of Otolaryngology, Head and Neck SurgeryDepartment of Radiation OncologyDepartment of Gastrointestinal surgeryDepartment of Otolaryngology, Head and Neck SurgeryOtorhinolaryngologyDepartment of Otolaryngology, Head and Neck SurgeryDepartment of Otolaryngology, Head and Neck SurgeryOrgan Transplant Management OfficeThe cancer centerDepartment of NeurosurgeryDepartment of Otolaryngology, Head and Neck SurgeryZhihe Lin,1,&ast; Chuntao Deng,2,&ast; Dan Wang,3,&ast; Bo Lyu,1 Jiaying Pan,4 Jinlian Li,1 Minting Chen,1 Zhaoxia Zhang,5 Zibin Liang,6 Lei Chen,7 Shaoyan Feng1 1Department of Otolaryngology, Head and Neck Surgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 2Department of Radiation Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 3Department of Gastrointestinal Surgery, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 4Department of Otolaryngology, Eye and ENT Hospital, ENT Institute, Fudan University, Zhuhai, Guangdong, People’s Republic of China; 5Office of Organ Transplant Management, The Fifth Affiliated Hospital, Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 6Department of Thoracic Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China; 7Department of Neurosurgery, The Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai, Guangdong, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Shaoyan Feng, Email fengshaoyan97@163.com; fshaoy@mail.sysu.edu.cn Lei Chen, Email clzzzz@126.comPurpose: This study investigates IL-33 and IL-25 expression in post-radiation otitis media with effusion (POME), along with classic oxidative stress markers (MDA and SOD), to investigate radiation-induced oxidative stress and its association with ILC2-mediated chronic inflammation, providing basis for targeted therapies.Methods: Middle ear effusions (MEE) were collected from 35 irradiated nasopharyngeal carcinoma patients with otitis media with effusion (POME, 43 ears) and 20 non-irradiated conventional chronic otitis media with effusion (CCOME, 20 ears) patients. IL-33, IL-25, IL-6, SOD, and MDA levels were measured by ELISA. Eustachian tube function was evaluated using the Endoscopic Evaluation of the Eustachian Tube (3ET) scoring system.Results: Comparative analysis revealed distinct molecular profiles between POME and CCOME, with POME showing significantly reduced IL-33 levels (p=0.046) but elevated SOD activity (p=0.015), along with a non-significant trend toward higher MDA (p=0.083). Temporal analysis demonstrated peak expression of both IL-25 and IL-33 at 6 months post-radiation. Correlation studies identified significant associations between IL-33 (r=0.391) and IL-6, as well as between IL-25 (r=0.483) and IL-6 (both p< 0.01). Clinically, IL-25 levels showed positive correlation with 3ET endoscopic scores (r=0.407, p=0.021) and were significantly reduced following tympanostomy tube placement (p=0.024). Notably, no direct correlation was observed between IL-33 and IL-25 (p> 0.05), nor were any significant associations found with allergic comorbidities (all p> 0.05).Conclusion: IL-33 and IL-25 synergistically drive ILC2-mediated chronic inflammation in radiation-induced OME, with IL-25 emerging as a biomarker for radiotherapy-associated Eustachian tube dysfunction. The 6-month cytokine surge post-radiation highlights a therapeutic window for targeted interventions to mitigate long-term complications. Keywords: post-irradiation, otitis media with effusion, nasopharyngeal carcinoma, interleukin-33, interleukin-25https://www.dovepress.com/expression-and-clinical-significance-of-il-33-and-il-25-in-post-irradi-peer-reviewed-fulltext-article-JIRpost-irradiationotitis media with effusionnasopharyngeal carcinomainterleukin-33interleukin-25. |
| spellingShingle | Lin Z Deng C Wang D Lyu B Pan J Li J Chen M Zhang Z Liang Z Chen L Feng S Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with Effusion Journal of Inflammation Research post-irradiation otitis media with effusion nasopharyngeal carcinoma interleukin-33 interleukin-25. |
| title | Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with Effusion |
| title_full | Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with Effusion |
| title_fullStr | Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with Effusion |
| title_full_unstemmed | Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with Effusion |
| title_short | Expression and Clinical Significance of IL-33 and IL-25 in Post-Irradiation Otitis Media with Effusion |
| title_sort | expression and clinical significance of il 33 and il 25 in post irradiation otitis media with effusion |
| topic | post-irradiation otitis media with effusion nasopharyngeal carcinoma interleukin-33 interleukin-25. |
| url | https://www.dovepress.com/expression-and-clinical-significance-of-il-33-and-il-25-in-post-irradi-peer-reviewed-fulltext-article-JIR |
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