Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS
Flammulina velutipes is the fourth largest edible fungus in China with high nutritional value. In this paper, ultrahigh-performance liquid chromatography tandem hybrid quadrupole-Orbitrap mass spectrometry (UPLC-Q-Exactive-Orbitrap MS) was used to identify the secondary metabolites of F. velutipes....
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2021/4103952 |
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| author | Ying Ding Sitan Chen Honglin Wang Shanlei Li Changyang Ma Jinmei Wang Lili Cui |
| author_facet | Ying Ding Sitan Chen Honglin Wang Shanlei Li Changyang Ma Jinmei Wang Lili Cui |
| author_sort | Ying Ding |
| collection | DOAJ |
| description | Flammulina velutipes is the fourth largest edible fungus in China with high nutritional value. In this paper, ultrahigh-performance liquid chromatography tandem hybrid quadrupole-Orbitrap mass spectrometry (UPLC-Q-Exactive-Orbitrap MS) was used to identify the secondary metabolites of F. velutipes. The metabolites were identified by comparing the retention time, accurate molecular weight, and MS2 data with standard databases of mzVault and mzCloud (compound: 17,000+) and BGI high-resolution accurate mass plant metabolome database (plant metabolite: 2500+). Finally, 26 secondary metabolites were preliminarily identified, including flavonoids, phenylpropanoids, organic acids, and steroids. |
| format | Article |
| id | doaj-art-c84b2265fe5645698f747e2ac7d0bf47 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-c84b2265fe5645698f747e2ac7d0bf472025-02-03T05:45:57ZengWileyJournal of Food Quality0146-94281745-45572021-01-01202110.1155/2021/41039524103952Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MSYing Ding0Sitan Chen1Honglin Wang2Shanlei Li3Changyang Ma4Jinmei Wang5Lili Cui6National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, Henan, ChinaFlammulina velutipes is the fourth largest edible fungus in China with high nutritional value. In this paper, ultrahigh-performance liquid chromatography tandem hybrid quadrupole-Orbitrap mass spectrometry (UPLC-Q-Exactive-Orbitrap MS) was used to identify the secondary metabolites of F. velutipes. The metabolites were identified by comparing the retention time, accurate molecular weight, and MS2 data with standard databases of mzVault and mzCloud (compound: 17,000+) and BGI high-resolution accurate mass plant metabolome database (plant metabolite: 2500+). Finally, 26 secondary metabolites were preliminarily identified, including flavonoids, phenylpropanoids, organic acids, and steroids.http://dx.doi.org/10.1155/2021/4103952 |
| spellingShingle | Ying Ding Sitan Chen Honglin Wang Shanlei Li Changyang Ma Jinmei Wang Lili Cui Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS Journal of Food Quality |
| title | Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS |
| title_full | Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS |
| title_fullStr | Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS |
| title_full_unstemmed | Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS |
| title_short | Identification of Secondary Metabolites in Flammulina velutipes by UPLC-Q-Exactive-Orbitrap MS |
| title_sort | identification of secondary metabolites in flammulina velutipes by uplc q exactive orbitrap ms |
| url | http://dx.doi.org/10.1155/2021/4103952 |
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