Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in Angiosarcoma
Cutaneous angiosarcoma is a rare and highly aggressive skin malignancy. The aim of this study is to explore the alteration of serum-derived extracellular vesicle (EV) in angiosarcoma patients and to evaluate its clinical utility as a novel circulating biomarker. In a microarray analysis to examine t...
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| Language: | English |
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Medical Journals Sweden
2025-02-01
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| Series: | Acta Dermato-Venereologica |
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| Online Access: | https://medicaljournalssweden.se/actadv/article/view/40902 |
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| author | Kazunori Yokoi Jing Wang Yusuke Yoshioka Yasuhiro Fujisawa Manabu Fujimoto Takahiro Ochiya Atsushi Tanemura |
| author_facet | Kazunori Yokoi Jing Wang Yusuke Yoshioka Yasuhiro Fujisawa Manabu Fujimoto Takahiro Ochiya Atsushi Tanemura |
| author_sort | Kazunori Yokoi |
| collection | DOAJ |
| description | Cutaneous angiosarcoma is a rare and highly aggressive skin malignancy. The aim of this study is to explore the alteration of serum-derived extracellular vesicle (EV) in angiosarcoma patients and to evaluate its clinical utility as a novel circulating biomarker. In a microarray analysis to examine the differential expression of specific EV-associated microRNAs in sera between cutaneous angiosarcoma patients and healthy controls, 73 microRNAs with significant upregulation and 100 microRNAs with significant downregulation, respectively, were identified in patients with angio-sarcoma. Among them, quantitative PCR confirmed that miR-184, miR-3925-5p, miR-3926, and miR-5703 were upregulated in sera of cutaneous angiosarcoma patients compared with those of healthy controls and melanoma patients. Additionally, these 4 microRNAs were expressed more highly in angiosarcoma cell lines compared with normal human endothelial cell lines and were prone to elevate along with disease progression. Furthermore, a gene analysis predicted that the target gene set of microRNAs might affect the regulation of TP53 via the epigenetic regulation of MECP2. Taken together, these 4 extracellular vesicle-associated microRNAs in circulation serve as a promising liquid biomarker to identify angiosarcoma patients and trace disease progression.
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| format | Article |
| id | doaj-art-c83d3851ad804a0a86fedb0efd462de7 |
| institution | OA Journals |
| issn | 0001-5555 1651-2057 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Medical Journals Sweden |
| record_format | Article |
| series | Acta Dermato-Venereologica |
| spelling | doaj-art-c83d3851ad804a0a86fedb0efd462de72025-08-20T02:03:35ZengMedical Journals SwedenActa Dermato-Venereologica0001-55551651-20572025-02-0110510.2340/actadv.v105.40902Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in AngiosarcomaKazunori Yokoi0https://orcid.org/0000-0003-0582-7325Jing Wang1Yusuke Yoshioka2Yasuhiro Fujisawa3Manabu Fujimoto4Takahiro Ochiya5Atsushi Tanemura6https://orcid.org/0000-0002-5239-8474Department of Dermatology, Osaka University Graduate School of Medicine, Osaka, JapanDepartment of Dermatology, Osaka University Graduate School of Medicine, Osaka, JapanDepartment of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo, JapanDepartment of Dermatology, Tsukuba University, Tsukuba, Ibaraki, JapanDepartment of Dermatology, Osaka University Graduate School of Medicine, Osaka, JapanDepartment of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo, JapanDepartment of Dermatology, Osaka University Graduate School of Medicine, Osaka, JapanCutaneous angiosarcoma is a rare and highly aggressive skin malignancy. The aim of this study is to explore the alteration of serum-derived extracellular vesicle (EV) in angiosarcoma patients and to evaluate its clinical utility as a novel circulating biomarker. In a microarray analysis to examine the differential expression of specific EV-associated microRNAs in sera between cutaneous angiosarcoma patients and healthy controls, 73 microRNAs with significant upregulation and 100 microRNAs with significant downregulation, respectively, were identified in patients with angio-sarcoma. Among them, quantitative PCR confirmed that miR-184, miR-3925-5p, miR-3926, and miR-5703 were upregulated in sera of cutaneous angiosarcoma patients compared with those of healthy controls and melanoma patients. Additionally, these 4 microRNAs were expressed more highly in angiosarcoma cell lines compared with normal human endothelial cell lines and were prone to elevate along with disease progression. Furthermore, a gene analysis predicted that the target gene set of microRNAs might affect the regulation of TP53 via the epigenetic regulation of MECP2. Taken together, these 4 extracellular vesicle-associated microRNAs in circulation serve as a promising liquid biomarker to identify angiosarcoma patients and trace disease progression. https://medicaljournalssweden.se/actadv/article/view/40902AngiosarcomaSerumExtracellular vesicleMicroRNABiomarker |
| spellingShingle | Kazunori Yokoi Jing Wang Yusuke Yoshioka Yasuhiro Fujisawa Manabu Fujimoto Takahiro Ochiya Atsushi Tanemura Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in Angiosarcoma Acta Dermato-Venereologica Angiosarcoma Serum Extracellular vesicle MicroRNA Biomarker |
| title | Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in Angiosarcoma |
| title_full | Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in Angiosarcoma |
| title_fullStr | Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in Angiosarcoma |
| title_full_unstemmed | Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in Angiosarcoma |
| title_short | Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in Angiosarcoma |
| title_sort | novel detection and clinical utility of serum derived extracellular vesicle in angiosarcoma |
| topic | Angiosarcoma Serum Extracellular vesicle MicroRNA Biomarker |
| url | https://medicaljournalssweden.se/actadv/article/view/40902 |
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