Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis
Tuberculosis (TB) is a serious global public health problem. Detecting the changes of adenosine deaminase (ADA), interleukin (IL)-1β, IL-2, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) released from macrophages and monocytes is greatly valuable in the diagnosis of TB. Hence, this study was...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2024-10-01
|
| Series: | Italian Journal of Medicine |
| Subjects: | |
| Online Access: | https://www.italjmed.org/ijm/article/view/1810 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850210067169673216 |
|---|---|
| author | Bui-Thi Thu Huong Nguyen Minh Hien Nguyen Tien Dung Le-Thi Minh Hien Luu Cong Thanh Luu Vu Dung Tran Khanh Chi Nguyen-Van Khiem Hoang Hai Yen |
| author_facet | Bui-Thi Thu Huong Nguyen Minh Hien Nguyen Tien Dung Le-Thi Minh Hien Luu Cong Thanh Luu Vu Dung Tran Khanh Chi Nguyen-Van Khiem Hoang Hai Yen |
| author_sort | Bui-Thi Thu Huong |
| collection | DOAJ |
| description |
Tuberculosis (TB) is a serious global public health problem. Detecting the changes of adenosine deaminase (ADA), interleukin (IL)-1β, IL-2, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) released from macrophages and monocytes is greatly valuable in the diagnosis of TB. Hence, this study was designed to determine the optimal cut-off points of ADA, IL-1β, IL-2, TNF-α, and IFN-γ, allowing to differentiate pleural TB (PLTB), peritoneal TB (PTB), and meningeal TB (MTB). The study included 386 patients with pleural effusion, 47 patients with peritoneal effusion, and 134 patients with encephalitis and meningoencephalitis suspect of TB. ADA was determined by an enzyme kinetics method; cytokine concentration was measured by a test based on the competitive enzyme-linked immunosorbent assay principle. Sensitivity, specificity, and positive and negative predictive value were calculated and described. In the aspiration fluid, ADA activity was greatly different between PLTB, PTB, and MTB groups with values of 37.5 U/L, 30.5 U/L, and 8.1 U/L and sensitivity and specificity of 87.7% and 83.4%; 100% and 88.9%; 97.3% and 98.6%, respectively. Similar, IFN-γ concentrations were distinctively different between PLTB, PTB, and MTB patients (with values of 120 pg/mL, 200 pg/mL, and 30 pg/mL with sensitivity and specificity of 80.3% and 80.9%; 80.0% and 92.6%; 98.6% and 61.7%, respectively). The determination of ADA and IFN-γ levels in the aspiration fluid is potentially considered a highly sensitive and specific test for differentiating PLTB, PTB, and MTB.
|
| format | Article |
| id | doaj-art-c83d16d8c74e41f7b86e3b69a05ae71b |
| institution | OA Journals |
| issn | 1877-9344 1877-9352 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Italian Journal of Medicine |
| spelling | doaj-art-c83d16d8c74e41f7b86e3b69a05ae71b2025-08-20T02:09:51ZengPAGEPress PublicationsItalian Journal of Medicine1877-93441877-93522024-10-0118310.4081/itjm.2024.1810Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosisBui-Thi Thu Huong0Nguyen Minh Hien1Nguyen Tien Dung2Le-Thi Minh Hien3Luu Cong Thanh4Luu Vu Dung5Tran Khanh Chi6Nguyen-Van Khiem7Hoang Hai Yen8Department of Biochemistry, Thai Nguyen University of Medicine and PharmacyDepartment of Biochemistry, Thanh Nhan Hospital, HanoiDepartment of Internal Medicine, Thai Nguyen University of Medicine and PharmacyDepartment of Biochemistry, Thai Nguyen University of Medicine and PharmacyDiagnostic Imaging Department, Tam Anh Hospital, HanoiDepartment of Biochemistry, Hai Phong Hospital of Obstetrics and GynecologyDepartment of Biochemistry, Ha Noi Medical UniversityDepartment of Pediatrics, Vietnam University of Traditional Medicine, HanoiGenetic Center, Phenikaa University Hospital (Phenikaamec), Hanoi Tuberculosis (TB) is a serious global public health problem. Detecting the changes of adenosine deaminase (ADA), interleukin (IL)-1β, IL-2, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ) released from macrophages and monocytes is greatly valuable in the diagnosis of TB. Hence, this study was designed to determine the optimal cut-off points of ADA, IL-1β, IL-2, TNF-α, and IFN-γ, allowing to differentiate pleural TB (PLTB), peritoneal TB (PTB), and meningeal TB (MTB). The study included 386 patients with pleural effusion, 47 patients with peritoneal effusion, and 134 patients with encephalitis and meningoencephalitis suspect of TB. ADA was determined by an enzyme kinetics method; cytokine concentration was measured by a test based on the competitive enzyme-linked immunosorbent assay principle. Sensitivity, specificity, and positive and negative predictive value were calculated and described. In the aspiration fluid, ADA activity was greatly different between PLTB, PTB, and MTB groups with values of 37.5 U/L, 30.5 U/L, and 8.1 U/L and sensitivity and specificity of 87.7% and 83.4%; 100% and 88.9%; 97.3% and 98.6%, respectively. Similar, IFN-γ concentrations were distinctively different between PLTB, PTB, and MTB patients (with values of 120 pg/mL, 200 pg/mL, and 30 pg/mL with sensitivity and specificity of 80.3% and 80.9%; 80.0% and 92.6%; 98.6% and 61.7%, respectively). The determination of ADA and IFN-γ levels in the aspiration fluid is potentially considered a highly sensitive and specific test for differentiating PLTB, PTB, and MTB. https://www.italjmed.org/ijm/article/view/1810ADAIL-1βIL-2TNF-αIFN-γcytokines |
| spellingShingle | Bui-Thi Thu Huong Nguyen Minh Hien Nguyen Tien Dung Le-Thi Minh Hien Luu Cong Thanh Luu Vu Dung Tran Khanh Chi Nguyen-Van Khiem Hoang Hai Yen Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis Italian Journal of Medicine ADA IL-1β IL-2 TNF-α IFN-γ cytokines |
| title | Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis |
| title_full | Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis |
| title_fullStr | Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis |
| title_full_unstemmed | Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis |
| title_short | Diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis |
| title_sort | diagnostic values of adenosine deaminase cytokines in fluid contributed to the detection of extrapulmonary tuberculosis |
| topic | ADA IL-1β IL-2 TNF-α IFN-γ cytokines |
| url | https://www.italjmed.org/ijm/article/view/1810 |
| work_keys_str_mv | AT buithithuhuong diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis AT nguyenminhhien diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis AT nguyentiendung diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis AT lethiminhhien diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis AT luucongthanh diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis AT luuvudung diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis AT trankhanhchi diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis AT nguyenvankhiem diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis AT hoanghaiyen diagnosticvaluesofadenosinedeaminasecytokinesinfluidcontributedtothedetectionofextrapulmonarytuberculosis |