Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances
Abstract Immobilizing enzymes onto solid supports having enhanced catalytic activity and resistance to harsh external conditions is considered as a promising and critical method of broadening enzymatic applications in biosensing, biocatalysis, and biomedical devices; however, it is considerably hamp...
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| Format: | Article |
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Wiley
2025-03-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202411196 |
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| author | Yiwen Wang Hongfei Tong Shulan Ni Kaiyuan Huo Wenjie Liu Xingjie Zan Xiaodie Yuan Shuangshuang Wang |
| author_facet | Yiwen Wang Hongfei Tong Shulan Ni Kaiyuan Huo Wenjie Liu Xingjie Zan Xiaodie Yuan Shuangshuang Wang |
| author_sort | Yiwen Wang |
| collection | DOAJ |
| description | Abstract Immobilizing enzymes onto solid supports having enhanced catalytic activity and resistance to harsh external conditions is considered as a promising and critical method of broadening enzymatic applications in biosensing, biocatalysis, and biomedical devices; however, it is considerably hampered by limited strategies. Here, a core–shell strategy involving a soft‐core hexahistidine metal assembly (HmA) is innovatively developed and characterized with encapsulated enzymes (catalase (CAT), horseradish peroxidase, glucose oxidase (GOx), and cascade enzymes (CAT+GOx)) and hard porous shells (zeolitic imidazolate framework (ZIF), ZIF‐8, ZIF‐67, ZIF‐90, calcium carbonate, and hydroxyapatite). The enzyme‐friendly environment provided by the embedded HmA proves beneficial for enhanced catalytic activity, which is particularly effective in preserving fragile enzymes that will have been deactivated without the HmA core during the mineralization of porous shells. The enzyme encapsulated within a core–shell particle exhibits noteworthy resilience against harsh external conditions, including heat, organic solvents, and proteinase K. Additionally, no significant alteration in the catalytic behavior of the enzyme is observed after multiple cycles of usage. This study offers a novel approach for immobilizing enzymes and rendering them resistant to harsh external conditions, with potential applications in diverse fields, including biocatalysis, bioremediation, and biomedical engineering. |
| format | Article |
| id | doaj-art-c832d22a2f0b4a90ac40a104ff031d8d |
| institution | OA Journals |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-c832d22a2f0b4a90ac40a104ff031d8d2025-08-20T02:35:40ZengWileyAdvanced Science2198-38442025-03-011210n/an/a10.1002/advs.202411196Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External DisturbancesYiwen Wang0Hongfei Tong1Shulan Ni2Kaiyuan Huo3Wenjie Liu4Xingjie Zan5Xiaodie Yuan6Shuangshuang Wang7Department of Cardiology The First People's Hospital of Wenling Wenling Hospital of Wenzhou Medical University Wenling Zhejiang 317500 ChinaYongkang First People's Hospital of Wenzhou Medical University Jinhua Zhejiang ChinaDepartment of Cardiology The First People's Hospital of Wenling Wenling Hospital of Wenzhou Medical University Wenling Zhejiang 317500 ChinaDepartment of Cardiology The First People's Hospital of Wenling Wenling Hospital of Wenzhou Medical University Wenling Zhejiang 317500 ChinaWenzhou Institute, University of Chinese Academy of Sciences Wenzhou Key Laboratory of Perioperative Medicine Wenzhou Zhejiang 325001 ChinaWenzhou Institute, University of Chinese Academy of Sciences Wenzhou Key Laboratory of Perioperative Medicine Wenzhou Zhejiang 325001 ChinaWenzhou Institute, University of Chinese Academy of Sciences Wenzhou Key Laboratory of Perioperative Medicine Wenzhou Zhejiang 325001 ChinaDepartment of Cardiology The First People's Hospital of Wenling Wenling Hospital of Wenzhou Medical University Wenling Zhejiang 317500 ChinaAbstract Immobilizing enzymes onto solid supports having enhanced catalytic activity and resistance to harsh external conditions is considered as a promising and critical method of broadening enzymatic applications in biosensing, biocatalysis, and biomedical devices; however, it is considerably hampered by limited strategies. Here, a core–shell strategy involving a soft‐core hexahistidine metal assembly (HmA) is innovatively developed and characterized with encapsulated enzymes (catalase (CAT), horseradish peroxidase, glucose oxidase (GOx), and cascade enzymes (CAT+GOx)) and hard porous shells (zeolitic imidazolate framework (ZIF), ZIF‐8, ZIF‐67, ZIF‐90, calcium carbonate, and hydroxyapatite). The enzyme‐friendly environment provided by the embedded HmA proves beneficial for enhanced catalytic activity, which is particularly effective in preserving fragile enzymes that will have been deactivated without the HmA core during the mineralization of porous shells. The enzyme encapsulated within a core–shell particle exhibits noteworthy resilience against harsh external conditions, including heat, organic solvents, and proteinase K. Additionally, no significant alteration in the catalytic behavior of the enzyme is observed after multiple cycles of usage. This study offers a novel approach for immobilizing enzymes and rendering them resistant to harsh external conditions, with potential applications in diverse fields, including biocatalysis, bioremediation, and biomedical engineering.https://doi.org/10.1002/advs.202411196catalysiscore–shell structureenzyme immobilizationhexahistidine metal assembly (HmA)zeolitic imidazolate framework (ZIF) |
| spellingShingle | Yiwen Wang Hongfei Tong Shulan Ni Kaiyuan Huo Wenjie Liu Xingjie Zan Xiaodie Yuan Shuangshuang Wang Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances Advanced Science catalysis core–shell structure enzyme immobilization hexahistidine metal assembly (HmA) zeolitic imidazolate framework (ZIF) |
| title | Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances |
| title_full | Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances |
| title_fullStr | Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances |
| title_full_unstemmed | Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances |
| title_short | Combining Hard Shell with Soft Core to Enhance Enzyme Activity and Resist External Disturbances |
| title_sort | combining hard shell with soft core to enhance enzyme activity and resist external disturbances |
| topic | catalysis core–shell structure enzyme immobilization hexahistidine metal assembly (HmA) zeolitic imidazolate framework (ZIF) |
| url | https://doi.org/10.1002/advs.202411196 |
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