Green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive RP-HPLC technique
Abstract A simple, selective, and environmentally sustainable RP-HPLC method was developed for simultaneous quantification of gabapentin (GAB) and Methylcobalamin (MET) in pharmaceutical products. Separation was achieved on a Zorbax Eclipse C8 column (150 × 4.6 mm, 3.5 μm) using a green mobile phase...
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Nature Portfolio
2025-06-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-07056-9 |
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| author | Samar M. Mahgoub Fahad M. Alminderej Ammena Y. Binsaleh Bandar R. Alsehli Sayed M. Saleh Mahmoud A. Mohamed |
| author_facet | Samar M. Mahgoub Fahad M. Alminderej Ammena Y. Binsaleh Bandar R. Alsehli Sayed M. Saleh Mahmoud A. Mohamed |
| author_sort | Samar M. Mahgoub |
| collection | DOAJ |
| description | Abstract A simple, selective, and environmentally sustainable RP-HPLC method was developed for simultaneous quantification of gabapentin (GAB) and Methylcobalamin (MET) in pharmaceutical products. Separation was achieved on a Zorbax Eclipse C8 column (150 × 4.6 mm, 3.5 μm) using a green mobile phase of potassium phosphate buffer (pH 6.9)/acetonitrile (95:5 v/v) at 2.0 mL/min, with detection at 210 nm. Total analysis time was 10 min with 100 µL injection volume. The method showed excellent linearity (3–50 µg/mL; R² > 0.9998), LODs (0.60–0.80 µg/mL), and LOQs (2.00–2.50 µg/mL). Environmental sustainability was validated by multiple metrics: AGREE (0.70), AGREEprep (0.71), and Analytical Eco-Scale (80), confirming superior greenness. Forced degradation studies demonstrated stability-indicating capability per ICH guidelines. This eco-friendly approach provides a reliable solution for quality control of GAB/MET formulations while minimizing environmental impact. |
| format | Article |
| id | doaj-art-c822e09d7d074cab81a459d359562098 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-c822e09d7d074cab81a459d3595620982025-08-20T03:31:45ZengNature PortfolioScientific Reports2045-23222025-06-0115111610.1038/s41598-025-07056-9Green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive RP-HPLC techniqueSamar M. Mahgoub0Fahad M. Alminderej1Ammena Y. Binsaleh2Bandar R. Alsehli3Sayed M. Saleh4Mahmoud A. Mohamed5Materials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef UniversityDepartment of Chemistry, College of Science, Qassim UniversityDepartment of Pharmacy Practice, College of Pharmacy, Princess Nourah bint Abdulrahman UniversityDepartment of Chemistry, Faculty of Science, Taibah UniversityDepartment of Chemistry, College of Science, Qassim UniversityHikma Pharmaceutical CompanyAbstract A simple, selective, and environmentally sustainable RP-HPLC method was developed for simultaneous quantification of gabapentin (GAB) and Methylcobalamin (MET) in pharmaceutical products. Separation was achieved on a Zorbax Eclipse C8 column (150 × 4.6 mm, 3.5 μm) using a green mobile phase of potassium phosphate buffer (pH 6.9)/acetonitrile (95:5 v/v) at 2.0 mL/min, with detection at 210 nm. Total analysis time was 10 min with 100 µL injection volume. The method showed excellent linearity (3–50 µg/mL; R² > 0.9998), LODs (0.60–0.80 µg/mL), and LOQs (2.00–2.50 µg/mL). Environmental sustainability was validated by multiple metrics: AGREE (0.70), AGREEprep (0.71), and Analytical Eco-Scale (80), confirming superior greenness. Forced degradation studies demonstrated stability-indicating capability per ICH guidelines. This eco-friendly approach provides a reliable solution for quality control of GAB/MET formulations while minimizing environmental impact.https://doi.org/10.1038/s41598-025-07056-9GabapentinMethylcobalaminGreen and white approachRP- HPLCStability |
| spellingShingle | Samar M. Mahgoub Fahad M. Alminderej Ammena Y. Binsaleh Bandar R. Alsehli Sayed M. Saleh Mahmoud A. Mohamed Green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive RP-HPLC technique Scientific Reports Gabapentin Methylcobalamin Green and white approach RP- HPLC Stability |
| title | Green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive RP-HPLC technique |
| title_full | Green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive RP-HPLC technique |
| title_fullStr | Green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive RP-HPLC technique |
| title_full_unstemmed | Green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive RP-HPLC technique |
| title_short | Green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive RP-HPLC technique |
| title_sort | green and white analytical approach for parallel quantification of gabapentin and methylcobalamin in medicinal products using inventive rp hplc technique |
| topic | Gabapentin Methylcobalamin Green and white approach RP- HPLC Stability |
| url | https://doi.org/10.1038/s41598-025-07056-9 |
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