Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying Ducks

This study investigated the effects of adding fermented mixed feed (FMF, composed of several unconventional protein feeds, such as brown rice, rice bran, rice bran meal, sunflower meal, cottonseed meal, and corn starch residue) into the diet of Longyan Shan-ma ducks on their egg quality and intestin...

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Main Authors: Changfeng Xiao, Yunying Xu, Changsuo Yang, Daqian He, Lihui Zhu
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Agriculture
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Online Access:https://www.mdpi.com/2077-0472/15/11/1230
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author Changfeng Xiao
Yunying Xu
Changsuo Yang
Daqian He
Lihui Zhu
author_facet Changfeng Xiao
Yunying Xu
Changsuo Yang
Daqian He
Lihui Zhu
author_sort Changfeng Xiao
collection DOAJ
description This study investigated the effects of adding fermented mixed feed (FMF, composed of several unconventional protein feeds, such as brown rice, rice bran, rice bran meal, sunflower meal, cottonseed meal, and corn starch residue) into the diet of Longyan Shan-ma ducks on their egg quality and intestinal health. The ducks were randomly divided into two groups: one group served as the control and received a standard diet, while the other group received a diet in which 4% of the feed was substituted with FMF. Compared to unfermented feed, FMF had elevated lactic acid levels and reduced phytic acid and crude fiber, along with higher amounts of crude protein and a range of amino acids, including serine, histidine, arginine, alanine, valine, methionine, cysteine, isoleucine, and lysine. FMF significantly enhanced egg production and improved the overall egg quality, such as eggshell strength and thickness. It also enhanced total antioxidant capacity and glutathione peroxidase concentrations in serum while reducing serum urea nitrogen and interleukin-1β levels. Histological analysis showed that FMF supplementation improved the ileal villus height-to-crypt depth ratio. Microbiota analysis demonstrated that FMF had a significant impact on β-diversity by increasing <i>Firmicutes</i>, <i>Actinobacteriota</i>, and <i>Desulfobacterota</i> and decreasing <i>Proteobacteria</i> and <i>Myxococcota</i> at the phylum level. The abundance of <i>Corynebacterium</i>, <i>Lactobacillus</i>, and <i>Gallicola</i> was found to be elevated due to FMF at the genus level, whereas <i>Kocuria</i>, <i>Rothia</i>, <i>Helicobacter</i>, and <i>Escherichia-Shigella</i> were decreased. Additionally, diets supplemented with FMF resulted in higher intestinal valeric acid levels among ducks. Our findings indicate that incorporating FMF into laying duck diets can enhance production performance, egg quality, and gut health.
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spelling doaj-art-c81febf13b6f4553bc2437ea1245969a2025-08-20T02:33:07ZengMDPI AGAgriculture2077-04722025-06-011511123010.3390/agriculture15111230Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying DucksChangfeng Xiao0Yunying Xu1Changsuo Yang2Daqian He3Lihui Zhu4Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaShanghai Nonghao Feed Co., Ltd., Shanghai 201501, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaInstitute of Animal Husbandry and Veterinary Science, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaThis study investigated the effects of adding fermented mixed feed (FMF, composed of several unconventional protein feeds, such as brown rice, rice bran, rice bran meal, sunflower meal, cottonseed meal, and corn starch residue) into the diet of Longyan Shan-ma ducks on their egg quality and intestinal health. The ducks were randomly divided into two groups: one group served as the control and received a standard diet, while the other group received a diet in which 4% of the feed was substituted with FMF. Compared to unfermented feed, FMF had elevated lactic acid levels and reduced phytic acid and crude fiber, along with higher amounts of crude protein and a range of amino acids, including serine, histidine, arginine, alanine, valine, methionine, cysteine, isoleucine, and lysine. FMF significantly enhanced egg production and improved the overall egg quality, such as eggshell strength and thickness. It also enhanced total antioxidant capacity and glutathione peroxidase concentrations in serum while reducing serum urea nitrogen and interleukin-1β levels. Histological analysis showed that FMF supplementation improved the ileal villus height-to-crypt depth ratio. Microbiota analysis demonstrated that FMF had a significant impact on β-diversity by increasing <i>Firmicutes</i>, <i>Actinobacteriota</i>, and <i>Desulfobacterota</i> and decreasing <i>Proteobacteria</i> and <i>Myxococcota</i> at the phylum level. The abundance of <i>Corynebacterium</i>, <i>Lactobacillus</i>, and <i>Gallicola</i> was found to be elevated due to FMF at the genus level, whereas <i>Kocuria</i>, <i>Rothia</i>, <i>Helicobacter</i>, and <i>Escherichia-Shigella</i> were decreased. Additionally, diets supplemented with FMF resulted in higher intestinal valeric acid levels among ducks. Our findings indicate that incorporating FMF into laying duck diets can enhance production performance, egg quality, and gut health.https://www.mdpi.com/2077-0472/15/11/1230fermented feedlaying duckegg qualityintestinal health
spellingShingle Changfeng Xiao
Yunying Xu
Changsuo Yang
Daqian He
Lihui Zhu
Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying Ducks
Agriculture
fermented feed
laying duck
egg quality
intestinal health
title Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying Ducks
title_full Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying Ducks
title_fullStr Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying Ducks
title_full_unstemmed Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying Ducks
title_short Fermented Mixed Feed Increased Egg Quality and Intestinal Health of Laying Ducks
title_sort fermented mixed feed increased egg quality and intestinal health of laying ducks
topic fermented feed
laying duck
egg quality
intestinal health
url https://www.mdpi.com/2077-0472/15/11/1230
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AT yunyingxu fermentedmixedfeedincreasedeggqualityandintestinalhealthoflayingducks
AT changsuoyang fermentedmixedfeedincreasedeggqualityandintestinalhealthoflayingducks
AT daqianhe fermentedmixedfeedincreasedeggqualityandintestinalhealthoflayingducks
AT lihuizhu fermentedmixedfeedincreasedeggqualityandintestinalhealthoflayingducks