An annotated near-complete sequence assembly of the Magnaporthe oryzae 70-15 reference genome
Abstract Magnaporthe oryzae is a devastating fungal pathogen that causes substantial yield losses in rice and other cereal crops worldwide. A high-quality genome assembly is critical for addressing challenges posed by this pathogen. However, the current widely used MG8 assembly of the M. oryzae stra...
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Nature Portfolio
2025-05-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05116-3 |
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| author | Hang-yuan Cheng Li-ping Jiang Yue Fei Fei Lu Shengwei Ma |
| author_facet | Hang-yuan Cheng Li-ping Jiang Yue Fei Fei Lu Shengwei Ma |
| author_sort | Hang-yuan Cheng |
| collection | DOAJ |
| description | Abstract Magnaporthe oryzae is a devastating fungal pathogen that causes substantial yield losses in rice and other cereal crops worldwide. A high-quality genome assembly is critical for addressing challenges posed by this pathogen. However, the current widely used MG8 assembly of the M. oryzae strain 70-15 reference genome contains numerous gaps and unresolved repetitive regions. Here, we report a complete 44.82 Mb high-quality nuclear genome and a 35.95 kb circular mitochondrial genome for strain 70-15, generated using deep-coverage PacBio high-fidelity sequencing (HiFi) and high-resolution chromatin conformation capture (Hi-C) data. Notably, we successfully resolved one or both telomere sequences for all seven chromosomes and achieved telomere-to-telomere (T2T) assemblies for chromosomes 2, 3, 4, 6, and 7. Based on this T2T assembly, we predicted 12,100 protein-coding genes and 493 effectors. This high-quality T2T assembly represents a significant advancement in M. oryzae genomics and provides an enhanced reference for studies in genome biology, comparative genomics, and population genetics of this economically important plant pathogen. |
| format | Article |
| id | doaj-art-c81d575886af4e0f96e380143e55ba34 |
| institution | DOAJ |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-c81d575886af4e0f96e380143e55ba342025-08-20T03:09:34ZengNature PortfolioScientific Data2052-44632025-05-011211910.1038/s41597-025-05116-3An annotated near-complete sequence assembly of the Magnaporthe oryzae 70-15 reference genomeHang-yuan Cheng0Li-ping Jiang1Yue Fei2Fei Lu3Shengwei Ma4Institute of Genetics and Developmental Biology, Chinese Academy of SciencesInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesInstitute of Genetics and Developmental Biology, Chinese Academy of SciencesAbstract Magnaporthe oryzae is a devastating fungal pathogen that causes substantial yield losses in rice and other cereal crops worldwide. A high-quality genome assembly is critical for addressing challenges posed by this pathogen. However, the current widely used MG8 assembly of the M. oryzae strain 70-15 reference genome contains numerous gaps and unresolved repetitive regions. Here, we report a complete 44.82 Mb high-quality nuclear genome and a 35.95 kb circular mitochondrial genome for strain 70-15, generated using deep-coverage PacBio high-fidelity sequencing (HiFi) and high-resolution chromatin conformation capture (Hi-C) data. Notably, we successfully resolved one or both telomere sequences for all seven chromosomes and achieved telomere-to-telomere (T2T) assemblies for chromosomes 2, 3, 4, 6, and 7. Based on this T2T assembly, we predicted 12,100 protein-coding genes and 493 effectors. This high-quality T2T assembly represents a significant advancement in M. oryzae genomics and provides an enhanced reference for studies in genome biology, comparative genomics, and population genetics of this economically important plant pathogen.https://doi.org/10.1038/s41597-025-05116-3 |
| spellingShingle | Hang-yuan Cheng Li-ping Jiang Yue Fei Fei Lu Shengwei Ma An annotated near-complete sequence assembly of the Magnaporthe oryzae 70-15 reference genome Scientific Data |
| title | An annotated near-complete sequence assembly of the Magnaporthe oryzae 70-15 reference genome |
| title_full | An annotated near-complete sequence assembly of the Magnaporthe oryzae 70-15 reference genome |
| title_fullStr | An annotated near-complete sequence assembly of the Magnaporthe oryzae 70-15 reference genome |
| title_full_unstemmed | An annotated near-complete sequence assembly of the Magnaporthe oryzae 70-15 reference genome |
| title_short | An annotated near-complete sequence assembly of the Magnaporthe oryzae 70-15 reference genome |
| title_sort | annotated near complete sequence assembly of the magnaporthe oryzae 70 15 reference genome |
| url | https://doi.org/10.1038/s41597-025-05116-3 |
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