Antimicrobial activity of reference Lactobacillus strains against WHO priority drug-resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysis
This study examines the antimicrobial properties of probiotic strains against twelve clinically relevant drug-resistant pathogens, including those classified as 'High priority' by the World Health Organization (WHO) for their significant antibiotic resistance. The probiotic strains, Lactob...
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Elsevier
2025-06-01
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| author | Vidhi Shah Richa Soni Milan Dabhi Dweipayan Goswami Rushikesh Joshi |
| author_facet | Vidhi Shah Richa Soni Milan Dabhi Dweipayan Goswami Rushikesh Joshi |
| author_sort | Vidhi Shah |
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| description | This study examines the antimicrobial properties of probiotic strains against twelve clinically relevant drug-resistant pathogens, including those classified as 'High priority' by the World Health Organization (WHO) for their significant antibiotic resistance. The probiotic strains, Lactobacillus acidophilus (LA, NCDC 13), Lacticaseibacillus casei (LC, NCDC 17), and Lactobacillus plantarum (LP, NCDC 20), were sourced from the National Dairy Research Institute (NDRI) in Karnal, India. Pathogens were isolated from clinical specimens, identified via Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS), and their antibiotic resistance profiles characterized using the VITEK® 2 system. The antibacterial effects of the probiotic strains were assessed using agar well diffusion and agar slab methods, demonstrating a spectrum of inhibitory activities against all twelve pathogens. Additionally, in silico genomic analysis was performed to explore the genetic basis of these antibacterial effects. Whole genome sequences of the American Type Culture Collection (ATCC) equivalents for the NDRI strains were traced and obtained from the National Center for Biotechnology Information (NCBI) database, then analyzed using BAGEL-4 to identify bacteriocin gene clusters. This analysis revealed the presence of diverse bacteriocin gene clusters, encoding bacteriocins such as Acidocin, Enterolysin A, Helveticin J, Lactococcin, Sakacin, and Enterocin X, which are known for their antimicrobial properties. These findings provide a genetic basis for the observed in vitro antibacterial activities, highlighting the potential of these Lactobacillus strains to inhibit drug-resistant pathogens. This integrated approach confirms the potential of the NDRI-derived Lactobacillus strains as alternative therapeutic agents against WHO-designated priority pathogens. |
| format | Article |
| id | doaj-art-c81b2b76ce7b49f3b030c7e566fd0786 |
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| language | English |
| publishDate | 2025-06-01 |
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| series | The Microbe |
| spelling | doaj-art-c81b2b76ce7b49f3b030c7e566fd07862025-08-20T02:30:03ZengElsevierThe Microbe2950-19462025-06-01710037810.1016/j.microb.2025.100378Antimicrobial activity of reference Lactobacillus strains against WHO priority drug-resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysisVidhi Shah0Richa Soni1Milan Dabhi2Dweipayan Goswami3Rushikesh Joshi4Department of Life Sciences, Food Science & Nutrition, University School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaDepartment of Life Sciences, Food Science & Nutrition, University School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, India; Corresponding author.Department of Microbiology & Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaDepartment of Microbiology & Biotechnology, University School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaDepartment of Biochemistry & Forensic Science, University School of Sciences, Gujarat University, Ahmedabad, Gujarat 380009, IndiaThis study examines the antimicrobial properties of probiotic strains against twelve clinically relevant drug-resistant pathogens, including those classified as 'High priority' by the World Health Organization (WHO) for their significant antibiotic resistance. The probiotic strains, Lactobacillus acidophilus (LA, NCDC 13), Lacticaseibacillus casei (LC, NCDC 17), and Lactobacillus plantarum (LP, NCDC 20), were sourced from the National Dairy Research Institute (NDRI) in Karnal, India. Pathogens were isolated from clinical specimens, identified via Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF-MS), and their antibiotic resistance profiles characterized using the VITEK® 2 system. The antibacterial effects of the probiotic strains were assessed using agar well diffusion and agar slab methods, demonstrating a spectrum of inhibitory activities against all twelve pathogens. Additionally, in silico genomic analysis was performed to explore the genetic basis of these antibacterial effects. Whole genome sequences of the American Type Culture Collection (ATCC) equivalents for the NDRI strains were traced and obtained from the National Center for Biotechnology Information (NCBI) database, then analyzed using BAGEL-4 to identify bacteriocin gene clusters. This analysis revealed the presence of diverse bacteriocin gene clusters, encoding bacteriocins such as Acidocin, Enterolysin A, Helveticin J, Lactococcin, Sakacin, and Enterocin X, which are known for their antimicrobial properties. These findings provide a genetic basis for the observed in vitro antibacterial activities, highlighting the potential of these Lactobacillus strains to inhibit drug-resistant pathogens. This integrated approach confirms the potential of the NDRI-derived Lactobacillus strains as alternative therapeutic agents against WHO-designated priority pathogens.http://www.sciencedirect.com/science/article/pii/S2950194625001463Antibacterial ActivityAnti-microbial Resistance (AMR)Gel Diffusion AssayWHO Priority PathogensReference Lactobacillus spp.Bacteriocin Gene Clusters |
| spellingShingle | Vidhi Shah Richa Soni Milan Dabhi Dweipayan Goswami Rushikesh Joshi Antimicrobial activity of reference Lactobacillus strains against WHO priority drug-resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysis The Microbe Antibacterial Activity Anti-microbial Resistance (AMR) Gel Diffusion Assay WHO Priority Pathogens Reference Lactobacillus spp. Bacteriocin Gene Clusters |
| title | Antimicrobial activity of reference Lactobacillus strains against WHO priority drug-resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysis |
| title_full | Antimicrobial activity of reference Lactobacillus strains against WHO priority drug-resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysis |
| title_fullStr | Antimicrobial activity of reference Lactobacillus strains against WHO priority drug-resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysis |
| title_full_unstemmed | Antimicrobial activity of reference Lactobacillus strains against WHO priority drug-resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysis |
| title_short | Antimicrobial activity of reference Lactobacillus strains against WHO priority drug-resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysis |
| title_sort | antimicrobial activity of reference lactobacillus strains against who priority drug resistant pathogens through an integrated in vitro and bacteriocin gene clusters analysis |
| topic | Antibacterial Activity Anti-microbial Resistance (AMR) Gel Diffusion Assay WHO Priority Pathogens Reference Lactobacillus spp. Bacteriocin Gene Clusters |
| url | http://www.sciencedirect.com/science/article/pii/S2950194625001463 |
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