Lycopene ameliorates diabetes-induced pancreatic, hepatic, and renal damage by modulating the JAK/STAT/SOCS signaling pathway in rats
Objective(s): Emerging evidence suggests that the JAK/STAT/SOCS signaling pathway is crucial for maintaining homeostasis, and its dysregulation contributes to diabetes development. This study aimed to evaluate the roles of SOCS-1 and SOCS-3 in the pancreas, liver, and kidney and to explore the invol...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2025-04-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_25548_5f629290e68b5b8a158b3b3c3a796b66.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849252027432960000 |
|---|---|
| author | Ozlem Ozmen Melda Şahin Senay Topsakal Şerife Taşan Uğur Şahi̇n |
| author_facet | Ozlem Ozmen Melda Şahin Senay Topsakal Şerife Taşan Uğur Şahi̇n |
| author_sort | Ozlem Ozmen |
| collection | DOAJ |
| description | Objective(s): Emerging evidence suggests that the JAK/STAT/SOCS signaling pathway is crucial for maintaining homeostasis, and its dysregulation contributes to diabetes development. This study aimed to evaluate the roles of SOCS-1 and SOCS-3 in the pancreas, liver, and kidney and to explore the involvement of the JAK/STAT pathway in the molecular mechanisms underlying their effects on inflammation and apoptosis, as well as organ injury in a diabetes mellitus (DM) model. Additionally, we sought to elucidate the role of the JAK/STAT/SOCS pathway in mediating the effects of lycopene (LYC).Materials and Methods: Forty Sprague-Dawley rats were divided into control, DM, LYC, and LYC+DM groups. Diabetes was induced in the DM groups using streptozotocin. LYC was administered to the LYC and LYC+DM groups for 30 days. After the study, pancreas, liver, and kidney tissues were analyzed using histopathological, immunohistochemical, and PCR methods.Results: Significant vacuolization and degenerative changes were observed in the DM group’s pancreatic islet cells. Kidney and liver tissues showed hyperemia, hemorrhage, and degenerative changes. Immunohistochemical analysis revealed increased expression of Cas-3, TNF-α, IFN-α, and IL-6, while IL-10 was significantly reduced in the DM group. PCR analysis showed elevated levels of TNF-α and Cas-3, with decreased SOCS-1 and SOCS-3 expression in the DM group.Conclusion: This study highlights the therapeutic potential of targeting the JAK/STAT/SOCS pathway with lycopene, demonstrating its promise in mitigating diabetes and related complications. |
| format | Article |
| id | doaj-art-c805549c0dee4a93a2e90f08afc035c9 |
| institution | Kabale University |
| issn | 2008-3866 2008-3874 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj-art-c805549c0dee4a93a2e90f08afc035c92025-08-20T03:56:45ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742025-04-0128446146810.22038/ijbms.2025.79979.1732625548Lycopene ameliorates diabetes-induced pancreatic, hepatic, and renal damage by modulating the JAK/STAT/SOCS signaling pathway in ratsOzlem Ozmen0Melda Şahin1Senay Topsakal2Şerife Taşan3Uğur Şahi̇n4Department of Pathology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, TürkiyeDepartment of Pathology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, TürkiyeDepartment of Endocrinology and Metabolism,University, Pamukkale Faculty of Medicine, Denizli, TürkiyeDepartment of Pathology, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, TürkiyeGenetic Research Unit, Innovative Technologies Application and Research Center, Süleyman Demirel University, Isparta, TürkiyeObjective(s): Emerging evidence suggests that the JAK/STAT/SOCS signaling pathway is crucial for maintaining homeostasis, and its dysregulation contributes to diabetes development. This study aimed to evaluate the roles of SOCS-1 and SOCS-3 in the pancreas, liver, and kidney and to explore the involvement of the JAK/STAT pathway in the molecular mechanisms underlying their effects on inflammation and apoptosis, as well as organ injury in a diabetes mellitus (DM) model. Additionally, we sought to elucidate the role of the JAK/STAT/SOCS pathway in mediating the effects of lycopene (LYC).Materials and Methods: Forty Sprague-Dawley rats were divided into control, DM, LYC, and LYC+DM groups. Diabetes was induced in the DM groups using streptozotocin. LYC was administered to the LYC and LYC+DM groups for 30 days. After the study, pancreas, liver, and kidney tissues were analyzed using histopathological, immunohistochemical, and PCR methods.Results: Significant vacuolization and degenerative changes were observed in the DM group’s pancreatic islet cells. Kidney and liver tissues showed hyperemia, hemorrhage, and degenerative changes. Immunohistochemical analysis revealed increased expression of Cas-3, TNF-α, IFN-α, and IL-6, while IL-10 was significantly reduced in the DM group. PCR analysis showed elevated levels of TNF-α and Cas-3, with decreased SOCS-1 and SOCS-3 expression in the DM group.Conclusion: This study highlights the therapeutic potential of targeting the JAK/STAT/SOCS pathway with lycopene, demonstrating its promise in mitigating diabetes and related complications.https://ijbms.mums.ac.ir/article_25548_5f629290e68b5b8a158b3b3c3a796b66.pdfapoptosiscytokinesdiabetes mellitusinflammationlycopenesignal transduction |
| spellingShingle | Ozlem Ozmen Melda Şahin Senay Topsakal Şerife Taşan Uğur Şahi̇n Lycopene ameliorates diabetes-induced pancreatic, hepatic, and renal damage by modulating the JAK/STAT/SOCS signaling pathway in rats Iranian Journal of Basic Medical Sciences apoptosis cytokines diabetes mellitus inflammation lycopene signal transduction |
| title | Lycopene ameliorates diabetes-induced pancreatic, hepatic, and renal damage by modulating the JAK/STAT/SOCS signaling pathway in rats |
| title_full | Lycopene ameliorates diabetes-induced pancreatic, hepatic, and renal damage by modulating the JAK/STAT/SOCS signaling pathway in rats |
| title_fullStr | Lycopene ameliorates diabetes-induced pancreatic, hepatic, and renal damage by modulating the JAK/STAT/SOCS signaling pathway in rats |
| title_full_unstemmed | Lycopene ameliorates diabetes-induced pancreatic, hepatic, and renal damage by modulating the JAK/STAT/SOCS signaling pathway in rats |
| title_short | Lycopene ameliorates diabetes-induced pancreatic, hepatic, and renal damage by modulating the JAK/STAT/SOCS signaling pathway in rats |
| title_sort | lycopene ameliorates diabetes induced pancreatic hepatic and renal damage by modulating the jak stat socs signaling pathway in rats |
| topic | apoptosis cytokines diabetes mellitus inflammation lycopene signal transduction |
| url | https://ijbms.mums.ac.ir/article_25548_5f629290e68b5b8a158b3b3c3a796b66.pdf |
| work_keys_str_mv | AT ozlemozmen lycopeneamelioratesdiabetesinducedpancreatichepaticandrenaldamagebymodulatingthejakstatsocssignalingpathwayinrats AT meldasahin lycopeneamelioratesdiabetesinducedpancreatichepaticandrenaldamagebymodulatingthejakstatsocssignalingpathwayinrats AT senaytopsakal lycopeneamelioratesdiabetesinducedpancreatichepaticandrenaldamagebymodulatingthejakstatsocssignalingpathwayinrats AT serifetasan lycopeneamelioratesdiabetesinducedpancreatichepaticandrenaldamagebymodulatingthejakstatsocssignalingpathwayinrats AT ugursahin lycopeneamelioratesdiabetesinducedpancreatichepaticandrenaldamagebymodulatingthejakstatsocssignalingpathwayinrats |