An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits
Objectives. To investigate whether botulinum toxin type A (BTXA) could control excessive secretion after submandibular gland (SMG) transplantation in rabbits and its possible mechanisms. Methods. A new SMG transplantation model was established in rabbit. 30 successfully constructed models were rando...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2016/7058537 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560764192292864 |
|---|---|
| author | Shang Xie Hui Xu Bo Lin Kan Wang Xiao-Feng Shan Zhi-Gang Cai |
| author_facet | Shang Xie Hui Xu Bo Lin Kan Wang Xiao-Feng Shan Zhi-Gang Cai |
| author_sort | Shang Xie |
| collection | DOAJ |
| description | Objectives. To investigate whether botulinum toxin type A (BTXA) could control excessive secretion after submandibular gland (SMG) transplantation in rabbits and its possible mechanisms. Methods. A new SMG transplantation model was established in rabbit. 30 successfully constructed models were randomly assigned to five groups including control group and four experimental groups. Secretion outputs were used to analyze the effect of BTXA injection on excessive secretion. Hematoxylin and eosin (HE) staining, transmission electron microscopy (TEM), Western blot, and immunofluorescence were performed to analyze its possible mechanisms. Results. After BTXA injection, a significant decrease of excessive secretion after SMG transplantation was found in 2 and 4 weeks groups, but no significant effect on 12 and 24 weeks groups. HE and TEM results showed that BTXA led to morphological and ultrastructural changes of acinar cells of transplanted SMG. Western blot results suggested that BTXA decreased the aquaporin-5 (AQP5) protein expression after BTXA injection for 2 and 4 weeks. Immunofluorescence results showed that AQP5 protein was mainly expressed in the cytoplasm after BTXA injection for 2 and 4 weeks, which might indicate that BTXA promoted AQP5 expression from the cell membrane to cytoplasm. Conclusion. BTXA could effectively control excessive secretion after SMG transplantation in rabbits. |
| format | Article |
| id | doaj-art-c8008c6a71d34ce2816cd905ec40bb6c |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-c8008c6a71d34ce2816cd905ec40bb6c2025-02-03T01:26:46ZengWileyJournal of Ophthalmology2090-004X2090-00582016-01-01201610.1155/2016/70585377058537An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in RabbitsShang Xie0Hui Xu1Bo Lin2Kan Wang3Xiao-Feng Shan4Zhi-Gang Cai5Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, No. 22 Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaObjectives. To investigate whether botulinum toxin type A (BTXA) could control excessive secretion after submandibular gland (SMG) transplantation in rabbits and its possible mechanisms. Methods. A new SMG transplantation model was established in rabbit. 30 successfully constructed models were randomly assigned to five groups including control group and four experimental groups. Secretion outputs were used to analyze the effect of BTXA injection on excessive secretion. Hematoxylin and eosin (HE) staining, transmission electron microscopy (TEM), Western blot, and immunofluorescence were performed to analyze its possible mechanisms. Results. After BTXA injection, a significant decrease of excessive secretion after SMG transplantation was found in 2 and 4 weeks groups, but no significant effect on 12 and 24 weeks groups. HE and TEM results showed that BTXA led to morphological and ultrastructural changes of acinar cells of transplanted SMG. Western blot results suggested that BTXA decreased the aquaporin-5 (AQP5) protein expression after BTXA injection for 2 and 4 weeks. Immunofluorescence results showed that AQP5 protein was mainly expressed in the cytoplasm after BTXA injection for 2 and 4 weeks, which might indicate that BTXA promoted AQP5 expression from the cell membrane to cytoplasm. Conclusion. BTXA could effectively control excessive secretion after SMG transplantation in rabbits.http://dx.doi.org/10.1155/2016/7058537 |
| spellingShingle | Shang Xie Hui Xu Bo Lin Kan Wang Xiao-Feng Shan Zhi-Gang Cai An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits Journal of Ophthalmology |
| title | An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits |
| title_full | An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits |
| title_fullStr | An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits |
| title_full_unstemmed | An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits |
| title_short | An Experimental Study on Botulinum Toxin Type A for the Treatment of Excessive Secretion after Submandibular Gland Transplantation in Rabbits |
| title_sort | experimental study on botulinum toxin type a for the treatment of excessive secretion after submandibular gland transplantation in rabbits |
| url | http://dx.doi.org/10.1155/2016/7058537 |
| work_keys_str_mv | AT shangxie anexperimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT huixu anexperimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT bolin anexperimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT kanwang anexperimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT xiaofengshan anexperimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT zhigangcai anexperimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT shangxie experimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT huixu experimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT bolin experimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT kanwang experimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT xiaofengshan experimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits AT zhigangcai experimentalstudyonbotulinumtoxintypeaforthetreatmentofexcessivesecretionaftersubmandibularglandtransplantationinrabbits |