EFFECT OF POST MORTEM INTERVAL TOWARD Β-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS
Background: The effects of post mortem interval on tryptase levels are unclear and have been challenging to quantify due to limited and at times contradictory research. Purpose: Analyze the effects of Post Mortem Interval (PMI) due to anaphylactic shock on the expression of β-tryptase and mast cell...
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Universitas Airlangga
2023-11-01
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| Series: | Journal of Vocational Health Studies |
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| Online Access: | https://e-journal.unair.ac.id/JVHS/article/view/41995 |
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| author | Imam Susilo Bilqisthi Ari Putra Ahmad Yudianto |
| author_facet | Imam Susilo Bilqisthi Ari Putra Ahmad Yudianto |
| author_sort | Imam Susilo |
| collection | DOAJ |
| description | Background: The effects of post mortem interval on tryptase levels are unclear and have been challenging to quantify due to limited and at times contradictory research. Purpose: Analyze the effects of Post Mortem Interval (PMI) due to anaphylactic shock on the expression of β-tryptase and mast cell chymase in the heart organ. Method: This type of research was an experimental study with a randomized block design (RBD) with the same subjects and a time series for 24 hours, using 5 (five) rabbits. The measurement of β-tryptase and chymase expression was based on immunohistochemical IRS (immunoreactive score) in the heart organs of rabbits experiencing anaphylactic shock sensitization and induction of anaphylactic shock using ovalbumin. Result: Based on the results of statistical tests using univariate analysis, there was a relationship between the length of death (post mortem interval) and the expression of mast cells β-Tryptase and cardiac chymase (p-value = 0.006) and chymase (p-value = 0.002) with (p-value <0.05). The effective test time with β-tryptase markers using cardiac organs was prone to long death time of 0 - 15 hours. The effective check-up time with chymase markers using the heart organ was at a long time of death of 9 hours. Conclusion: The results of the study showed that tryptase and chymase could be used as markers of anaphylactic shock. |
| format | Article |
| id | doaj-art-c7fbeb8a83e4423f9554d2bb862f94d6 |
| institution | Kabale University |
| issn | 2580-7161 2580-717X |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal of Vocational Health Studies |
| spelling | doaj-art-c7fbeb8a83e4423f9554d2bb862f94d62025-02-03T06:01:31ZengUniversitas AirlanggaJournal of Vocational Health Studies2580-71612580-717X2023-11-017210711410.20473/jvhs.V7.I2.2023.107-11440076EFFECT OF POST MORTEM INTERVAL TOWARD Β-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLSImam Susilo0https://orcid.org/0000-0003-3194-2853Bilqisthi Ari Putra1https://orcid.org/0000-0002-4167-1957Ahmad Yudianto2https://orcid.org/0000-0003-4754-768XDepartment of Pathology, Medical Faculty of Universitas Airlangga/ Dr. Soetomo General Departement of Forensic, Graduate School of Universitas AirlanggaDepartement of Forensic, Graduate School of Universitas AirlanggaBackground: The effects of post mortem interval on tryptase levels are unclear and have been challenging to quantify due to limited and at times contradictory research. Purpose: Analyze the effects of Post Mortem Interval (PMI) due to anaphylactic shock on the expression of β-tryptase and mast cell chymase in the heart organ. Method: This type of research was an experimental study with a randomized block design (RBD) with the same subjects and a time series for 24 hours, using 5 (five) rabbits. The measurement of β-tryptase and chymase expression was based on immunohistochemical IRS (immunoreactive score) in the heart organs of rabbits experiencing anaphylactic shock sensitization and induction of anaphylactic shock using ovalbumin. Result: Based on the results of statistical tests using univariate analysis, there was a relationship between the length of death (post mortem interval) and the expression of mast cells β-Tryptase and cardiac chymase (p-value = 0.006) and chymase (p-value = 0.002) with (p-value <0.05). The effective test time with β-tryptase markers using cardiac organs was prone to long death time of 0 - 15 hours. The effective check-up time with chymase markers using the heart organ was at a long time of death of 9 hours. Conclusion: The results of the study showed that tryptase and chymase could be used as markers of anaphylactic shock.https://e-journal.unair.ac.id/JVHS/article/view/41995anaphylactic shockβ-tryptasechymaserabbitheart organ |
| spellingShingle | Imam Susilo Bilqisthi Ari Putra Ahmad Yudianto EFFECT OF POST MORTEM INTERVAL TOWARD Β-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS Journal of Vocational Health Studies anaphylactic shock β-tryptase chymase rabbit heart organ |
| title | EFFECT OF POST MORTEM INTERVAL TOWARD Β-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS |
| title_full | EFFECT OF POST MORTEM INTERVAL TOWARD Β-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS |
| title_fullStr | EFFECT OF POST MORTEM INTERVAL TOWARD Β-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS |
| title_full_unstemmed | EFFECT OF POST MORTEM INTERVAL TOWARD Β-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS |
| title_short | EFFECT OF POST MORTEM INTERVAL TOWARD Β-TRYPTASE AND CHYMASE EXPRESSION OF ANAPHYLACTIC HEART MAST CELLS |
| title_sort | effect of post mortem interval toward β tryptase and chymase expression of anaphylactic heart mast cells |
| topic | anaphylactic shock β-tryptase chymase rabbit heart organ |
| url | https://e-journal.unair.ac.id/JVHS/article/view/41995 |
| work_keys_str_mv | AT imamsusilo effectofpostmortemintervaltowardbtryptaseandchymaseexpressionofanaphylacticheartmastcells AT bilqisthiariputra effectofpostmortemintervaltowardbtryptaseandchymaseexpressionofanaphylacticheartmastcells AT ahmadyudianto effectofpostmortemintervaltowardbtryptaseandchymaseexpressionofanaphylacticheartmastcells |