miR-21 Responsive Nanocarrier Targeting Ovarian Cancer Cells
In recent years, DNA origami-based nanocarriers have been extensively utilized for efficient cancer therapy. However, developing a nanocarrier capable of effectively protecting cargos such as RNA remains a challenge. In this study, we designed a compact and controllable DNA tubular origami (DTO) mea...
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| Language: | English |
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Elsevier
2024-12-01
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| Series: | Computational and Structural Biotechnology Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S200103702400045X |
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| author | Liting Han Tao Song Xinyu Wang Yan Luo Chuanqi Gu Xin Li Jinda Wen Zhibin Wen Xiaolong Shi |
| author_facet | Liting Han Tao Song Xinyu Wang Yan Luo Chuanqi Gu Xin Li Jinda Wen Zhibin Wen Xiaolong Shi |
| author_sort | Liting Han |
| collection | DOAJ |
| description | In recent years, DNA origami-based nanocarriers have been extensively utilized for efficient cancer therapy. However, developing a nanocarrier capable of effectively protecting cargos such as RNA remains a challenge. In this study, we designed a compact and controllable DNA tubular origami (DTO) measuring 120 nm in length and 18 nm in width. The DTO exhibited appropriate structural characteristics for encapsulating and safeguarding cargo. Inside the DTO, we incorporated 20 connecting points to facilitate the delivery of cargoes to various ovarian and normal epithelial cell lines. Specifically, fluorescent-labeled DNA strands were attached to these sites as cargoes. The DTO was engineered to open upon encountering miR-21 through RNA/DNA strand displacement. Significantly, for the first time, we inhibited fluorescence using the compact DNA nanotube and observed dynamic fluorescent signals, indicating the controllable opening of DTO through live-cell imaging. Our results demonstrated that the DTO remained properly closed, exhibited effective internalization in ovarian cancer cells in vitro, showcasing marked differential expression of miR-21, and efficiently opened with short-term exposure to miR-21. Leveraging its autonomous behavior and compact design, the DTO emerges as a promising nanocarrier for various clinically relevant materials. It holds significant application prospects in anti-cancer therapy and the development of flexible biosensors. |
| format | Article |
| id | doaj-art-c7fb5cbf699e4c09b3445b156cfa9f9e |
| institution | OA Journals |
| issn | 2001-0370 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Computational and Structural Biotechnology Journal |
| spelling | doaj-art-c7fb5cbf699e4c09b3445b156cfa9f9e2025-08-20T02:37:24ZengElsevierComputational and Structural Biotechnology Journal2001-03702024-12-012419620410.1016/j.csbj.2024.02.021miR-21 Responsive Nanocarrier Targeting Ovarian Cancer CellsLiting Han0Tao Song1Xinyu Wang2Yan Luo3Chuanqi Gu4Xin Li5Jinda Wen6Zhibin Wen7Xiaolong Shi8Department of Gynecology 2, Renmin Hospital of Wuhan University, Wuhan, ChinaCollege of Computer Science and Technology, China University of Petroleum (East China), Qingdao, ChinaDepartment of Gynecology 2, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Gynecology 2, Renmin Hospital of Wuhan University, Wuhan, ChinaDepartment of Gynecology 2, Renmin Hospital of Wuhan University, Wuhan, China; College of Computer Science and Technology, China University of Petroleum (East China), Qingdao, China; Institute of Computing Science and Technology, Guangzhou University, Guangzhou, ChinaDepartment of Gynecology 2, Renmin Hospital of Wuhan University, Wuhan, China; Corresponding author.Institute of Computing Science and Technology, Guangzhou University, Guangzhou, ChinaInstitute of Computing Science and Technology, Guangzhou University, Guangzhou, ChinaInstitute of Computing Science and Technology, Guangzhou University, Guangzhou, ChinaIn recent years, DNA origami-based nanocarriers have been extensively utilized for efficient cancer therapy. However, developing a nanocarrier capable of effectively protecting cargos such as RNA remains a challenge. In this study, we designed a compact and controllable DNA tubular origami (DTO) measuring 120 nm in length and 18 nm in width. The DTO exhibited appropriate structural characteristics for encapsulating and safeguarding cargo. Inside the DTO, we incorporated 20 connecting points to facilitate the delivery of cargoes to various ovarian and normal epithelial cell lines. Specifically, fluorescent-labeled DNA strands were attached to these sites as cargoes. The DTO was engineered to open upon encountering miR-21 through RNA/DNA strand displacement. Significantly, for the first time, we inhibited fluorescence using the compact DNA nanotube and observed dynamic fluorescent signals, indicating the controllable opening of DTO through live-cell imaging. Our results demonstrated that the DTO remained properly closed, exhibited effective internalization in ovarian cancer cells in vitro, showcasing marked differential expression of miR-21, and efficiently opened with short-term exposure to miR-21. Leveraging its autonomous behavior and compact design, the DTO emerges as a promising nanocarrier for various clinically relevant materials. It holds significant application prospects in anti-cancer therapy and the development of flexible biosensors.http://www.sciencedirect.com/science/article/pii/S200103702400045XDNA origamiNano-drug3D nanotubeMiR-21Ovarian cancerCancer therapy |
| spellingShingle | Liting Han Tao Song Xinyu Wang Yan Luo Chuanqi Gu Xin Li Jinda Wen Zhibin Wen Xiaolong Shi miR-21 Responsive Nanocarrier Targeting Ovarian Cancer Cells Computational and Structural Biotechnology Journal DNA origami Nano-drug 3D nanotube MiR-21 Ovarian cancer Cancer therapy |
| title | miR-21 Responsive Nanocarrier Targeting Ovarian Cancer Cells |
| title_full | miR-21 Responsive Nanocarrier Targeting Ovarian Cancer Cells |
| title_fullStr | miR-21 Responsive Nanocarrier Targeting Ovarian Cancer Cells |
| title_full_unstemmed | miR-21 Responsive Nanocarrier Targeting Ovarian Cancer Cells |
| title_short | miR-21 Responsive Nanocarrier Targeting Ovarian Cancer Cells |
| title_sort | mir 21 responsive nanocarrier targeting ovarian cancer cells |
| topic | DNA origami Nano-drug 3D nanotube MiR-21 Ovarian cancer Cancer therapy |
| url | http://www.sciencedirect.com/science/article/pii/S200103702400045X |
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