Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-forming Escherichia coli O157:H7 strain in co-culture biofilms
This study evaluated the impact of meat-processing environmental bacteria (MPB) on biofilm formation by Escherichia coli O157:H7 in dual-species cultures. Biofilm development by 50 MPB and E. coli O157:H7 was assessed using crystal violet staining. Four MPB and E. coli O157:H7 combinations were eval...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1517732/full |
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| author | Jeyachchandran Visvalingam Peipei Zhang Xianqin Yang |
| author_facet | Jeyachchandran Visvalingam Peipei Zhang Xianqin Yang |
| author_sort | Jeyachchandran Visvalingam |
| collection | DOAJ |
| description | This study evaluated the impact of meat-processing environmental bacteria (MPB) on biofilm formation by Escherichia coli O157:H7 in dual-species cultures. Biofilm development by 50 MPB and E. coli O157:H7 was assessed using crystal violet staining. Four MPB and E. coli O157:H7 combinations were evaluated further for viable cell numbers. A chlorinated alkaline agent and a quaternary ammonium-based agent were evaluated for their ability to remove biofilms. The E. coli O157:H7 strain was a non-biofilm former. In dual-species biofilms, if the companion MPB did not produce detectable biofilm, then the pairing did not produce measurable biofilms either. The interaction effect between MPB and E. coli O157:H7 was predominantly no-effect (neutral). Among the four MPB isolates tested by viable cell enumeration method, only generic E. coli genotype 136 reduced viable numbers of E. coli O157:H7 in dual-strain biofilm. Sequential treatment with cleaning and sanitizing treatment provided a better removal of biofilm than a single-agent treatment. |
| format | Article |
| id | doaj-art-c7f8a3fb534047de86820dd4f2138c9a |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-c7f8a3fb534047de86820dd4f2138c9a2025-08-20T02:13:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011510.3389/fmicb.2024.15177321517732Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-forming Escherichia coli O157:H7 strain in co-culture biofilmsJeyachchandran VisvalingamPeipei ZhangXianqin YangThis study evaluated the impact of meat-processing environmental bacteria (MPB) on biofilm formation by Escherichia coli O157:H7 in dual-species cultures. Biofilm development by 50 MPB and E. coli O157:H7 was assessed using crystal violet staining. Four MPB and E. coli O157:H7 combinations were evaluated further for viable cell numbers. A chlorinated alkaline agent and a quaternary ammonium-based agent were evaluated for their ability to remove biofilms. The E. coli O157:H7 strain was a non-biofilm former. In dual-species biofilms, if the companion MPB did not produce detectable biofilm, then the pairing did not produce measurable biofilms either. The interaction effect between MPB and E. coli O157:H7 was predominantly no-effect (neutral). Among the four MPB isolates tested by viable cell enumeration method, only generic E. coli genotype 136 reduced viable numbers of E. coli O157:H7 in dual-strain biofilm. Sequential treatment with cleaning and sanitizing treatment provided a better removal of biofilm than a single-agent treatment.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1517732/fullEscherichia coliE. coli O157biofilmmeat-processing environmental bacteriasanitation |
| spellingShingle | Jeyachchandran Visvalingam Peipei Zhang Xianqin Yang Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-forming Escherichia coli O157:H7 strain in co-culture biofilms Frontiers in Microbiology Escherichia coli E. coli O157 biofilm meat-processing environmental bacteria sanitation |
| title | Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-forming Escherichia coli O157:H7 strain in co-culture biofilms |
| title_full | Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-forming Escherichia coli O157:H7 strain in co-culture biofilms |
| title_fullStr | Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-forming Escherichia coli O157:H7 strain in co-culture biofilms |
| title_full_unstemmed | Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-forming Escherichia coli O157:H7 strain in co-culture biofilms |
| title_short | Inter- and intra-species interactions between meat plant environmental bacteria and a non-biofilm-forming Escherichia coli O157:H7 strain in co-culture biofilms |
| title_sort | inter and intra species interactions between meat plant environmental bacteria and a non biofilm forming escherichia coli o157 h7 strain in co culture biofilms |
| topic | Escherichia coli E. coli O157 biofilm meat-processing environmental bacteria sanitation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1517732/full |
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