Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay
The chances of accidental exposure are augmented as the application of ionizing radiation increases in various fields. Such accidental exposures may occur at nuclear power plants, laboratories, and hospitals. Cytogenetic assays have been used for estimating radiation dose in the situation of the a...
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The Ecological Society of Korea
2014-12-01
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| Series: | Journal of Ecology and Environment |
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| Online Access: | http://dx.doi.org/10.5141/ecoenv.2014.023 |
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| author | Tae Ho Ryu Jin-Hong Kim Jin Kyu Kim |
| author_facet | Tae Ho Ryu Jin-Hong Kim Jin Kyu Kim |
| author_sort | Tae Ho Ryu |
| collection | DOAJ |
| description | The chances of accidental exposure are augmented as the application of ionizing radiation increases in various fields.
Such accidental exposures may occur at nuclear power plants, laboratories, and hospitals. Cytogenetic assays have been
used for estimating radiation dose in the situation of the accidents. The micronucleus assay has several advantages over
the other cytogenetic methods as it is simple and fast. The present study aimed at investigation of the micronuclei frequencies
in cytokinesis-block cells in human blood lymphocytes after γ-irradiation and at establishment of a standard
dose response relationship. The samples of peripheral blood were obtained from 6 different donors aged between 24 and
30 years old. The bloods were irradiated in vitro with 0-5 Gy. A linear quadratic dose-response equation was obtained by
scoring the micronuclei in binucleated cells; y = 27.87x2 + 46.13x + 2.08 (r2 = 0.99). Irradiation caused a significant decrease
in the nuclear division index. Necrotic and apoptotic cells increased in number after irradiation in a dose-dependent
manner. In conclusion, the conventional cytokinesis-block micronucleus assay has proven to be the great technique in
biological dosimetry. Dose-response calibration curve derived from CMBN assay could be used to estimate the exposure
dose during a radiological emergency. |
| format | Article |
| id | doaj-art-c7f1d1b499014a1eabf36bb85a50bf96 |
| institution | OA Journals |
| issn | 2287-8327 2288-1220 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | The Ecological Society of Korea |
| record_format | Article |
| series | Journal of Ecology and Environment |
| spelling | doaj-art-c7f1d1b499014a1eabf36bb85a50bf962025-08-20T02:23:53ZengThe Ecological Society of KoreaJournal of Ecology and Environment2287-83272288-12202014-12-0137419520010.5141/ecoenv.2014.023Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assayTae Ho Ryu0Jin-Hong Kim1Jin Kyu Kim2Korea Atomic Energy Research Institute (KAERI), Advanced Radiation Technology, Jeongeup 580-185, Koera1Korea Atomic Energy Research Institute (KAERI), Advanced Radiation Technology, Jeongeup 580-185, Koera1Korea Atomic Energy Research Institute (KAERI), Advanced Radiation Technology, Jeongeup 580-185, KoeraThe chances of accidental exposure are augmented as the application of ionizing radiation increases in various fields. Such accidental exposures may occur at nuclear power plants, laboratories, and hospitals. Cytogenetic assays have been used for estimating radiation dose in the situation of the accidents. The micronucleus assay has several advantages over the other cytogenetic methods as it is simple and fast. The present study aimed at investigation of the micronuclei frequencies in cytokinesis-block cells in human blood lymphocytes after γ-irradiation and at establishment of a standard dose response relationship. The samples of peripheral blood were obtained from 6 different donors aged between 24 and 30 years old. The bloods were irradiated in vitro with 0-5 Gy. A linear quadratic dose-response equation was obtained by scoring the micronuclei in binucleated cells; y = 27.87x2 + 46.13x + 2.08 (r2 = 0.99). Irradiation caused a significant decrease in the nuclear division index. Necrotic and apoptotic cells increased in number after irradiation in a dose-dependent manner. In conclusion, the conventional cytokinesis-block micronucleus assay has proven to be the great technique in biological dosimetry. Dose-response calibration curve derived from CMBN assay could be used to estimate the exposure dose during a radiological emergency.http://dx.doi.org/10.5141/ecoenv.2014.023biodosimetryCBMN assayhuman lymphocytesionizing radiation |
| spellingShingle | Tae Ho Ryu Jin-Hong Kim Jin Kyu Kim Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay Journal of Ecology and Environment biodosimetry CBMN assay human lymphocytes ionizing radiation |
| title | Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay |
| title_full | Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay |
| title_fullStr | Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay |
| title_full_unstemmed | Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay |
| title_short | Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay |
| title_sort | radiation exposure dose in human blood lymphocytes as assessed by the cbmn assay |
| topic | biodosimetry CBMN assay human lymphocytes ionizing radiation |
| url | http://dx.doi.org/10.5141/ecoenv.2014.023 |
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