Radiation exposure dose in human blood lymphocytes as assessed by the CBMN assay
The chances of accidental exposure are augmented as the application of ionizing radiation increases in various fields. Such accidental exposures may occur at nuclear power plants, laboratories, and hospitals. Cytogenetic assays have been used for estimating radiation dose in the situation of the a...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
The Ecological Society of Korea
2014-12-01
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| Series: | Journal of Ecology and Environment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.5141/ecoenv.2014.023 |
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| Summary: | The chances of accidental exposure are augmented as the application of ionizing radiation increases in various fields.
Such accidental exposures may occur at nuclear power plants, laboratories, and hospitals. Cytogenetic assays have been
used for estimating radiation dose in the situation of the accidents. The micronucleus assay has several advantages over
the other cytogenetic methods as it is simple and fast. The present study aimed at investigation of the micronuclei frequencies
in cytokinesis-block cells in human blood lymphocytes after γ-irradiation and at establishment of a standard
dose response relationship. The samples of peripheral blood were obtained from 6 different donors aged between 24 and
30 years old. The bloods were irradiated in vitro with 0-5 Gy. A linear quadratic dose-response equation was obtained by
scoring the micronuclei in binucleated cells; y = 27.87x2 + 46.13x + 2.08 (r2 = 0.99). Irradiation caused a significant decrease
in the nuclear division index. Necrotic and apoptotic cells increased in number after irradiation in a dose-dependent
manner. In conclusion, the conventional cytokinesis-block micronucleus assay has proven to be the great technique in
biological dosimetry. Dose-response calibration curve derived from CMBN assay could be used to estimate the exposure
dose during a radiological emergency. |
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| ISSN: | 2287-8327 2288-1220 |