Perioperative fluid therapy impairs lymphatic pump function in male rats
Abstract Because of its life‐saving benefits, perioperative IV fluid therapy remains a cornerstone of medical treatment. However, it also induces sustained edemagenic stress. The resulting persistent interstitial edema—excessive fluid accumulation in the interstitium—significantly delays recovery an...
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| Format: | Article |
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Wiley
2025-06-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.70389 |
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| author | Rebecca C. Harlow‐Adamek Reetu Singh Randolph H. Stewart Cristine L. Heaps Glen A. Laine Charles S. Cox Jr. Ranjeet M. Dongaonkar |
| author_facet | Rebecca C. Harlow‐Adamek Reetu Singh Randolph H. Stewart Cristine L. Heaps Glen A. Laine Charles S. Cox Jr. Ranjeet M. Dongaonkar |
| author_sort | Rebecca C. Harlow‐Adamek |
| collection | DOAJ |
| description | Abstract Because of its life‐saving benefits, perioperative IV fluid therapy remains a cornerstone of medical treatment. However, it also induces sustained edemagenic stress. The resulting persistent interstitial edema—excessive fluid accumulation in the interstitium—significantly delays recovery and worsens patient outcomes. Therefore, to gain a detailed understanding of the lymphatic functional consequences of perioperative fluid therapy, this study aimed to test the hypothesis that perioperative IV fluid therapy compromises lymphatic pump function within 3 days after major surgery. Following a midline laparotomy, animals received IV fluid therapy over 48 h during recovery (FLTP). Three days post‐surgery, mesenteric lymphatic vessels from FLTP and sham surgery (CTRL) animals were isolated, and lymphatic pump function was assessed in vitro. The transmural pressure‐pump flow and circumferential length‐wall tension relationships of FLTP vessels were altered—contraction frequency and normalized pump flow and active and passive wall tensions were significantly lower than CTRL. In vessels from another group of animals with surgically produced mesenteric venous hypertension to induce sustained edemagenic stress, only the pressure‐pump flow relationship was altered similarly to FLTP. These results demonstrate the detrimental effects of perioperative fluid therapy on lymphatic pumping, which is essential for restoring interstitial fluid pressure and resolving edema and inflammation. |
| format | Article |
| id | doaj-art-c7ebb1412ff3444f95f819411c651f2f |
| institution | DOAJ |
| issn | 2051-817X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj-art-c7ebb1412ff3444f95f819411c651f2f2025-08-20T03:20:56ZengWileyPhysiological Reports2051-817X2025-06-011311n/an/a10.14814/phy2.70389Perioperative fluid therapy impairs lymphatic pump function in male ratsRebecca C. Harlow‐Adamek0Reetu Singh1Randolph H. Stewart2Cristine L. Heaps3Glen A. Laine4Charles S. Cox Jr.5Ranjeet M. Dongaonkar6Michael E. DeBakey Institute for Comparative Cardiovascular Science and Biomedical Devices, Department of Veterinary Physiology & Pharmacology Texas A&M University College Station Texas USAMichael E. DeBakey Institute for Comparative Cardiovascular Science and Biomedical Devices, Department of Veterinary Physiology & Pharmacology Texas A&M University College Station Texas USAMichael E. DeBakey Institute for Comparative Cardiovascular Science and Biomedical Devices, Department of Veterinary Physiology & Pharmacology Texas A&M University College Station Texas USAMichael E. DeBakey Institute for Comparative Cardiovascular Science and Biomedical Devices, Department of Veterinary Physiology & Pharmacology Texas A&M University College Station Texas USAMichael E. DeBakey Institute for Comparative Cardiovascular Science and Biomedical Devices, Department of Veterinary Physiology & Pharmacology Texas A&M University College Station Texas USADepartment of Pediatric Surgery The University of Texas Medical School Houston Texas USAMichael E. DeBakey Institute for Comparative Cardiovascular Science and Biomedical Devices, Department of Veterinary Physiology & Pharmacology Texas A&M University College Station Texas USAAbstract Because of its life‐saving benefits, perioperative IV fluid therapy remains a cornerstone of medical treatment. However, it also induces sustained edemagenic stress. The resulting persistent interstitial edema—excessive fluid accumulation in the interstitium—significantly delays recovery and worsens patient outcomes. Therefore, to gain a detailed understanding of the lymphatic functional consequences of perioperative fluid therapy, this study aimed to test the hypothesis that perioperative IV fluid therapy compromises lymphatic pump function within 3 days after major surgery. Following a midline laparotomy, animals received IV fluid therapy over 48 h during recovery (FLTP). Three days post‐surgery, mesenteric lymphatic vessels from FLTP and sham surgery (CTRL) animals were isolated, and lymphatic pump function was assessed in vitro. The transmural pressure‐pump flow and circumferential length‐wall tension relationships of FLTP vessels were altered—contraction frequency and normalized pump flow and active and passive wall tensions were significantly lower than CTRL. In vessels from another group of animals with surgically produced mesenteric venous hypertension to induce sustained edemagenic stress, only the pressure‐pump flow relationship was altered similarly to FLTP. These results demonstrate the detrimental effects of perioperative fluid therapy on lymphatic pumping, which is essential for restoring interstitial fluid pressure and resolving edema and inflammation.https://doi.org/10.14814/phy2.70389enhanced recovery after surgerygoal‐directed fluid therapygut edemaintestinal dysfunctionresuscitation |
| spellingShingle | Rebecca C. Harlow‐Adamek Reetu Singh Randolph H. Stewart Cristine L. Heaps Glen A. Laine Charles S. Cox Jr. Ranjeet M. Dongaonkar Perioperative fluid therapy impairs lymphatic pump function in male rats Physiological Reports enhanced recovery after surgery goal‐directed fluid therapy gut edema intestinal dysfunction resuscitation |
| title | Perioperative fluid therapy impairs lymphatic pump function in male rats |
| title_full | Perioperative fluid therapy impairs lymphatic pump function in male rats |
| title_fullStr | Perioperative fluid therapy impairs lymphatic pump function in male rats |
| title_full_unstemmed | Perioperative fluid therapy impairs lymphatic pump function in male rats |
| title_short | Perioperative fluid therapy impairs lymphatic pump function in male rats |
| title_sort | perioperative fluid therapy impairs lymphatic pump function in male rats |
| topic | enhanced recovery after surgery goal‐directed fluid therapy gut edema intestinal dysfunction resuscitation |
| url | https://doi.org/10.14814/phy2.70389 |
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