CLASP promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in Arabidopsis meristematic cells.
Following cytokinesis in plants, Endoplasmic MTs (EMTs) assemble on the nuclear surface, forming a radial network that extends out to the cell cortex, where they attach and incorporate into the cortical microtubule (CMT) array. We found that in these post-cytokinetic cells, the MT-associated protein...
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| _version_ | 1850231108810047488 |
|---|---|
| author | P Yen Le Chris Ambrose |
| author_facet | P Yen Le Chris Ambrose |
| author_sort | P Yen Le |
| collection | DOAJ |
| description | Following cytokinesis in plants, Endoplasmic MTs (EMTs) assemble on the nuclear surface, forming a radial network that extends out to the cell cortex, where they attach and incorporate into the cortical microtubule (CMT) array. We found that in these post-cytokinetic cells, the MT-associated protein CLASP is enriched at sites of EMT-cortex attachment, and is required for stable EMT tethering and growth into the cell cortex. Loss of EMT-cortex anchoring in clasp-1 mutants results in destabilized EMT arrays, and is accompanied by enhanced mobility of the cytoplasm, premature vacuolation, and precocious entry into cell elongation phase. Thus, EMTs appear to maintain cells in a meristematic state by providing a structural scaffold that stabilizes the cytoplasm to counteract actomyosin-based cytoplasmic streaming forces, thereby preventing premature establishment of a central vacuole and rapid cell elongation. |
| format | Article |
| id | doaj-art-c7e72e4b83314dac85d14902f6dcc96c |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-c7e72e4b83314dac85d14902f6dcc96c2025-08-20T02:03:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019852110.1371/journal.pone.0198521CLASP promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in Arabidopsis meristematic cells.P Yen LeChris AmbroseFollowing cytokinesis in plants, Endoplasmic MTs (EMTs) assemble on the nuclear surface, forming a radial network that extends out to the cell cortex, where they attach and incorporate into the cortical microtubule (CMT) array. We found that in these post-cytokinetic cells, the MT-associated protein CLASP is enriched at sites of EMT-cortex attachment, and is required for stable EMT tethering and growth into the cell cortex. Loss of EMT-cortex anchoring in clasp-1 mutants results in destabilized EMT arrays, and is accompanied by enhanced mobility of the cytoplasm, premature vacuolation, and precocious entry into cell elongation phase. Thus, EMTs appear to maintain cells in a meristematic state by providing a structural scaffold that stabilizes the cytoplasm to counteract actomyosin-based cytoplasmic streaming forces, thereby preventing premature establishment of a central vacuole and rapid cell elongation.https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198521/1/pone.0198521.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210218%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210218T195535Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
| spellingShingle | P Yen Le Chris Ambrose CLASP promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in Arabidopsis meristematic cells. PLoS ONE |
| title | CLASP promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in Arabidopsis meristematic cells. |
| title_full | CLASP promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in Arabidopsis meristematic cells. |
| title_fullStr | CLASP promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in Arabidopsis meristematic cells. |
| title_full_unstemmed | CLASP promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in Arabidopsis meristematic cells. |
| title_short | CLASP promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in Arabidopsis meristematic cells. |
| title_sort | clasp promotes stable tethering of endoplasmic microtubules to the cell cortex to maintain cytoplasmic stability in arabidopsis meristematic cells |
| url | https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198521/1/pone.0198521.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210218%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210218T195535Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
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