Interrelationships of the Intestinal Microbiome, Trimethylamine N-Oxide and Lipopolysaccharide-Binding Protein with Crohn’s Disease Activity
Crohn’s disease (CD) is a multifactorial inflammatory bowel disease whose pathogenetic mechanisms are a field of ongoing study. Changes in the intestinal microbiome in CD may influence metabolite production and reflect the disease’s severity. We investigate the relationship between trimethylamine N-...
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2024-12-01
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| author | Yelena Laryushina Nadezhda Samoilova-Bedych Lyudmila Turgunova Alexandr Marchenko Yermek Turgunov Samat Kozhakhmetov Maxat Suieubayev Nurislam Mukhanbetzhanov Nadezhda Kabdulina |
| author_facet | Yelena Laryushina Nadezhda Samoilova-Bedych Lyudmila Turgunova Alexandr Marchenko Yermek Turgunov Samat Kozhakhmetov Maxat Suieubayev Nurislam Mukhanbetzhanov Nadezhda Kabdulina |
| author_sort | Yelena Laryushina |
| collection | DOAJ |
| description | Crohn’s disease (CD) is a multifactorial inflammatory bowel disease whose pathogenetic mechanisms are a field of ongoing study. Changes in the intestinal microbiome in CD may influence metabolite production and reflect the disease’s severity. We investigate the relationship between trimethylamine N-oxide (TMAO) and lipopolysaccharide-binding protein (LPS) levels and changes in the gut microbiome in patients with CD of various degrees of activity. Methods: In total, 29 CD patients and 15 healthy individuals were investigated for their levels of TMAO by HPLC-MS, and LPS protein by ELISA and metagenomic 16 s-sequencing of feces was performed. Results: We found significant differences in TMAO levels in patients in the remission/mild and moderate/severe groups compared to the control group (<i>p</i> = 0.02 and <i>p</i> = 0.014), changes in alpha diversity with the Shannon index (<i>p</i> = 0. 0151 and <i>p</i> = 0.0018) and in beta diversity (ANOSIM <i>p</i> = 0.009 and PERMANOVA <i>p</i> = 0.005) in both groups compared to controls. Strongly positive correlations in TMAO levels and mixed correlations of LPS with alpha diversity metrics were found, as well as significant correlations with microbiota species. Conclusions: Changes in the level of metabolites may reflect specific disturbances in the composition of the intestinal microbiome at different degrees of severity of CD. |
| format | Article |
| id | doaj-art-c7e085e08185492783fb9bcfa84cb821 |
| institution | Kabale University |
| issn | 2076-0817 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj-art-c7e085e08185492783fb9bcfa84cb8212025-01-24T13:44:34ZengMDPI AGPathogens2076-08172024-12-01141510.3390/pathogens14010005Interrelationships of the Intestinal Microbiome, Trimethylamine N-Oxide and Lipopolysaccharide-Binding Protein with Crohn’s Disease ActivityYelena Laryushina0Nadezhda Samoilova-Bedych1Lyudmila Turgunova2Alexandr Marchenko3Yermek Turgunov4Samat Kozhakhmetov5Maxat Suieubayev6Nurislam Mukhanbetzhanov7Nadezhda Kabdulina8Department of Internal Diseases, Karaganda Medical University, Karaganda 100000, KazakhstanDepartment of Internal Diseases, Karaganda Medical University, Karaganda 100000, KazakhstanDepartment of Internal Diseases, Karaganda Medical University, Karaganda 100000, KazakhstanDepartment of Internal Diseases, Karaganda Medical University, Karaganda 100000, KazakhstanDepartment of Internal Diseases, Karaganda Medical University, Karaganda 100000, KazakhstanNational Laboratory Astana, Nazarbayev University, Astana 010000, KazakhstanNational Laboratory Astana, Nazarbayev University, Astana 010000, KazakhstanNational Laboratory Astana, Nazarbayev University, Astana 010000, KazakhstanGastroenterology Department, Regional Clinical Hospital, Karaganda 100000, KazakhstanCrohn’s disease (CD) is a multifactorial inflammatory bowel disease whose pathogenetic mechanisms are a field of ongoing study. Changes in the intestinal microbiome in CD may influence metabolite production and reflect the disease’s severity. We investigate the relationship between trimethylamine N-oxide (TMAO) and lipopolysaccharide-binding protein (LPS) levels and changes in the gut microbiome in patients with CD of various degrees of activity. Methods: In total, 29 CD patients and 15 healthy individuals were investigated for their levels of TMAO by HPLC-MS, and LPS protein by ELISA and metagenomic 16 s-sequencing of feces was performed. Results: We found significant differences in TMAO levels in patients in the remission/mild and moderate/severe groups compared to the control group (<i>p</i> = 0.02 and <i>p</i> = 0.014), changes in alpha diversity with the Shannon index (<i>p</i> = 0. 0151 and <i>p</i> = 0.0018) and in beta diversity (ANOSIM <i>p</i> = 0.009 and PERMANOVA <i>p</i> = 0.005) in both groups compared to controls. Strongly positive correlations in TMAO levels and mixed correlations of LPS with alpha diversity metrics were found, as well as significant correlations with microbiota species. Conclusions: Changes in the level of metabolites may reflect specific disturbances in the composition of the intestinal microbiome at different degrees of severity of CD.https://www.mdpi.com/2076-0817/14/1/5Crohn’s diseaseCDIBDtrimethylamine N-oxidelipopolysaccharide-binding proteinTMAO |
| spellingShingle | Yelena Laryushina Nadezhda Samoilova-Bedych Lyudmila Turgunova Alexandr Marchenko Yermek Turgunov Samat Kozhakhmetov Maxat Suieubayev Nurislam Mukhanbetzhanov Nadezhda Kabdulina Interrelationships of the Intestinal Microbiome, Trimethylamine N-Oxide and Lipopolysaccharide-Binding Protein with Crohn’s Disease Activity Pathogens Crohn’s disease CD IBD trimethylamine N-oxide lipopolysaccharide-binding protein TMAO |
| title | Interrelationships of the Intestinal Microbiome, Trimethylamine N-Oxide and Lipopolysaccharide-Binding Protein with Crohn’s Disease Activity |
| title_full | Interrelationships of the Intestinal Microbiome, Trimethylamine N-Oxide and Lipopolysaccharide-Binding Protein with Crohn’s Disease Activity |
| title_fullStr | Interrelationships of the Intestinal Microbiome, Trimethylamine N-Oxide and Lipopolysaccharide-Binding Protein with Crohn’s Disease Activity |
| title_full_unstemmed | Interrelationships of the Intestinal Microbiome, Trimethylamine N-Oxide and Lipopolysaccharide-Binding Protein with Crohn’s Disease Activity |
| title_short | Interrelationships of the Intestinal Microbiome, Trimethylamine N-Oxide and Lipopolysaccharide-Binding Protein with Crohn’s Disease Activity |
| title_sort | interrelationships of the intestinal microbiome trimethylamine n oxide and lipopolysaccharide binding protein with crohn s disease activity |
| topic | Crohn’s disease CD IBD trimethylamine N-oxide lipopolysaccharide-binding protein TMAO |
| url | https://www.mdpi.com/2076-0817/14/1/5 |
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