Exploring Skin Longevity Pathways: <i>Rosa hybrid</i> Extract-Mediated AMP-Activated Protein Kinase Activation, Antioxidant, and Autophagic Mechanisms in Human Keratinocytes
The skin is continuously exposed to environmental changes, rendering it vulnerable to damage from external stressors that contribute to premature skin aging. This study aims to explore skin longevity pathways stimulated by a rose extract (RE) derived from petals. Human keratinocytes treated with RE...
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MDPI AG
2025-03-01
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| Series: | Cosmetics |
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| author | Olivier Gouin Andrea Cavagnino Gayané Azadiguian Sibylle Jäger Gilles Comte Mohammed Bendahmane Lionel Breton Martin A. Baraibar Annie F. Black |
| author_facet | Olivier Gouin Andrea Cavagnino Gayané Azadiguian Sibylle Jäger Gilles Comte Mohammed Bendahmane Lionel Breton Martin A. Baraibar Annie F. Black |
| author_sort | Olivier Gouin |
| collection | DOAJ |
| description | The skin is continuously exposed to environmental changes, rendering it vulnerable to damage from external stressors that contribute to premature skin aging. This study aims to explore skin longevity pathways stimulated by a rose extract (RE) derived from petals. Human keratinocytes treated with RE exhibited a significant increase in NRF2 (NF-E2-related factor 2; ≈2–4% of induction) and LAMP2A (Lysosome-Associated Membrane Protein 2A; ≈6–12% of induction) levels. The presence of RE significantly mitigated the increase in carbonylation levels (≈34–37% of protection) and the number of labeled P16<sup>INK4A</sup> cells (≈60–72% of protection), associated with proliferation arrest, both induced by exposure to BAP (Benzo[<i>a</i>]pyrene) coupled with UV-A (Ultraviolet A) irradiation. The beneficial effects mediated by RE were inhibited by Compound C, a specific AMPK inhibitor (AMP-activated protein kinase). The involvement of the AMPK pathway in mediating the beneficial effects of RE has been confirmed by assessing its activation through the evaluation of its phosphorylation state which was significantly elevated in the presence of RE compared to the stress condition. In conclusion, the activation of the AMPK pathway enhances antioxidant defenses and promotes autophagy. This dual action, mediated by RE, helps protect skin cells from oxidative damage and senescence while maintaining proteostasis, skin integrity, and cellular proliferation under pollution-induced stress (BAP + UV-A). These findings highlight the potential in mitigating age-related skin changes through the modulation of longevity pathways. |
| format | Article |
| id | doaj-art-c7dcfa6c22f24a9689bd7cacda40d664 |
| institution | DOAJ |
| issn | 2079-9284 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Cosmetics |
| spelling | doaj-art-c7dcfa6c22f24a9689bd7cacda40d6642025-08-20T03:13:51ZengMDPI AGCosmetics2079-92842025-03-011225710.3390/cosmetics12020057Exploring Skin Longevity Pathways: <i>Rosa hybrid</i> Extract-Mediated AMP-Activated Protein Kinase Activation, Antioxidant, and Autophagic Mechanisms in Human KeratinocytesOlivier Gouin0Andrea Cavagnino1Gayané Azadiguian2Sibylle Jäger3Gilles Comte4Mohammed Bendahmane5Lionel Breton6Martin A. Baraibar7Annie F. Black8Oxiproteomics, 2 Rue Antoine Etex, 94000 Créteil, FranceOxiproteomics, 2 Rue Antoine Etex, 94000 Créteil, FranceLancôme, 62 Quai Charles Pasqua, 92300 Levallois Perret, FranceL’Oréal Recherche & Innovation, 1 Av. Eugène Schueller, 93600 Aulnay-sous-Bois, FranceUMR Ecologie Microbienne, CNRS, INRAE, VetAgro Sup, UCBL, Université de Lyon, 69007 Lyon, FranceLaboratoire Reproduction et Développement des Plantes, INRAE-CNRS-Lyon, Ecole Normale Supérieure de Lyon, 69007 Lyon, FranceCILIA Consulting, 18 Av de Paris, 78000 Versailles, FranceOxiproteomics, 2 Rue Antoine Etex, 94000 Créteil, FranceLancôme, 62 Quai Charles Pasqua, 92300 Levallois Perret, FranceThe skin is continuously exposed to environmental changes, rendering it vulnerable to damage from external stressors that contribute to premature skin aging. This study aims to explore skin longevity pathways stimulated by a rose extract (RE) derived from petals. Human keratinocytes treated with RE exhibited a significant increase in NRF2 (NF-E2-related factor 2; ≈2–4% of induction) and LAMP2A (Lysosome-Associated Membrane Protein 2A; ≈6–12% of induction) levels. The presence of RE significantly mitigated the increase in carbonylation levels (≈34–37% of protection) and the number of labeled P16<sup>INK4A</sup> cells (≈60–72% of protection), associated with proliferation arrest, both induced by exposure to BAP (Benzo[<i>a</i>]pyrene) coupled with UV-A (Ultraviolet A) irradiation. The beneficial effects mediated by RE were inhibited by Compound C, a specific AMPK inhibitor (AMP-activated protein kinase). The involvement of the AMPK pathway in mediating the beneficial effects of RE has been confirmed by assessing its activation through the evaluation of its phosphorylation state which was significantly elevated in the presence of RE compared to the stress condition. In conclusion, the activation of the AMPK pathway enhances antioxidant defenses and promotes autophagy. This dual action, mediated by RE, helps protect skin cells from oxidative damage and senescence while maintaining proteostasis, skin integrity, and cellular proliferation under pollution-induced stress (BAP + UV-A). These findings highlight the potential in mitigating age-related skin changes through the modulation of longevity pathways.https://www.mdpi.com/2079-9284/12/2/57rose extractautophagysenescenceAMPKcarbonylationproteostasis |
| spellingShingle | Olivier Gouin Andrea Cavagnino Gayané Azadiguian Sibylle Jäger Gilles Comte Mohammed Bendahmane Lionel Breton Martin A. Baraibar Annie F. Black Exploring Skin Longevity Pathways: <i>Rosa hybrid</i> Extract-Mediated AMP-Activated Protein Kinase Activation, Antioxidant, and Autophagic Mechanisms in Human Keratinocytes Cosmetics rose extract autophagy senescence AMPK carbonylation proteostasis |
| title | Exploring Skin Longevity Pathways: <i>Rosa hybrid</i> Extract-Mediated AMP-Activated Protein Kinase Activation, Antioxidant, and Autophagic Mechanisms in Human Keratinocytes |
| title_full | Exploring Skin Longevity Pathways: <i>Rosa hybrid</i> Extract-Mediated AMP-Activated Protein Kinase Activation, Antioxidant, and Autophagic Mechanisms in Human Keratinocytes |
| title_fullStr | Exploring Skin Longevity Pathways: <i>Rosa hybrid</i> Extract-Mediated AMP-Activated Protein Kinase Activation, Antioxidant, and Autophagic Mechanisms in Human Keratinocytes |
| title_full_unstemmed | Exploring Skin Longevity Pathways: <i>Rosa hybrid</i> Extract-Mediated AMP-Activated Protein Kinase Activation, Antioxidant, and Autophagic Mechanisms in Human Keratinocytes |
| title_short | Exploring Skin Longevity Pathways: <i>Rosa hybrid</i> Extract-Mediated AMP-Activated Protein Kinase Activation, Antioxidant, and Autophagic Mechanisms in Human Keratinocytes |
| title_sort | exploring skin longevity pathways i rosa hybrid i extract mediated amp activated protein kinase activation antioxidant and autophagic mechanisms in human keratinocytes |
| topic | rose extract autophagy senescence AMPK carbonylation proteostasis |
| url | https://www.mdpi.com/2079-9284/12/2/57 |
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