Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues
ObjectiveTo analyze the differences of free and esterified oxo-eicosatetraenoic acids (oxo-ETEs) in blood cells and plasma from arterial and venous blood in hemodialysis (HD) patients. MethodsArterial and venous blood samples from 12 patients with end-stage renal disease (ESRD) before and after HD t...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Shanghai Chinese Clinical Medicine Press Co., Ltd.
2025-02-01
|
| Series: | Zhongguo Linchuang Yixue |
| Subjects: | |
| Online Access: | https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20241213 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850180350545756160 |
|---|---|
| author | Tong LIU Gollasch Maik C. Luft Friedrich Pan LIN Jun JI Yao MENG |
| author_facet | Tong LIU Gollasch Maik C. Luft Friedrich Pan LIN Jun JI Yao MENG |
| author_sort | Tong LIU |
| collection | DOAJ |
| description | ObjectiveTo analyze the differences of free and esterified oxo-eicosatetraenoic acids (oxo-ETEs) in blood cells and plasma from arterial and venous blood in hemodialysis (HD) patients. MethodsArterial and venous blood samples from 12 patients with end-stage renal disease (ESRD) before and after HD treatment at Charité – Universitätsmedizin Berlin, Germany, from June to December 2020 were collected. The esterified and free oxo-ETEs derived from arachidonic acid in blood cells and plasma were measured by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). ResultsNeither esterified nor free oxo-ETEs in blood cells displayed significant arteriovenous differences before and after HD. HD predominantly affected the metabolic levels of esterified and free oxo-ETEs in plasma. HD reduced the arteriovenous differences of esterified 12-oxo-ETE, free 15-oxo-ETE, and free 5-oxo-ETE in plasma, while raised the arteriovenous differences of esterified 15-oxo-ETE. ConclusionsThe oxo-ETEs in blood cells are relatively well-stabilized responding to HD treatment, whereas arteriovenous differences of free and esterified oxo-ETEs in plasma are present and active in response to HD treatment, potentially contributing to the cardiovascular disease. |
| format | Article |
| id | doaj-art-c7db631421004ac68ff8ee705b87af2c |
| institution | OA Journals |
| issn | 1008-6358 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Shanghai Chinese Clinical Medicine Press Co., Ltd. |
| record_format | Article |
| series | Zhongguo Linchuang Yixue |
| spelling | doaj-art-c7db631421004ac68ff8ee705b87af2c2025-08-20T02:18:14ZengShanghai Chinese Clinical Medicine Press Co., Ltd.Zhongguo Linchuang Yixue1008-63582025-02-013219310010.12025/j.issn.1008-6358.2025.2024121320241213Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissuesTong LIU0Gollasch Maik1C. Luft Friedrich2Pan LIN3Jun JI4Yao MENG5Department of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Internal Medicine and Geriatrics, University Medicine Greifswald, Greifswald 17475, GermanyExperimental and Clinical Research Center, a joint institution of Charité Medical Faculty and Max Delbrück Center (MDC) for Molecular Medicine, Berlin 13125, GermanyDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaDepartment of Nephrology, Zhongshan Hospital, Fudan University, Shanghai 200032, ChinaObjectiveTo analyze the differences of free and esterified oxo-eicosatetraenoic acids (oxo-ETEs) in blood cells and plasma from arterial and venous blood in hemodialysis (HD) patients. MethodsArterial and venous blood samples from 12 patients with end-stage renal disease (ESRD) before and after HD treatment at Charité – Universitätsmedizin Berlin, Germany, from June to December 2020 were collected. The esterified and free oxo-ETEs derived from arachidonic acid in blood cells and plasma were measured by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). ResultsNeither esterified nor free oxo-ETEs in blood cells displayed significant arteriovenous differences before and after HD. HD predominantly affected the metabolic levels of esterified and free oxo-ETEs in plasma. HD reduced the arteriovenous differences of esterified 12-oxo-ETE, free 15-oxo-ETE, and free 5-oxo-ETE in plasma, while raised the arteriovenous differences of esterified 15-oxo-ETE. ConclusionsThe oxo-ETEs in blood cells are relatively well-stabilized responding to HD treatment, whereas arteriovenous differences of free and esterified oxo-ETEs in plasma are present and active in response to HD treatment, potentially contributing to the cardiovascular disease.https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20241213hemodialysiseicosanoidsoxo-eicosatetraenoic acidbiotransformationarteriovenous difference |
| spellingShingle | Tong LIU Gollasch Maik C. Luft Friedrich Pan LIN Jun JI Yao MENG Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues Zhongguo Linchuang Yixue hemodialysis eicosanoids oxo-eicosatetraenoic acid biotransformation arteriovenous difference |
| title | Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues |
| title_full | Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues |
| title_fullStr | Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues |
| title_full_unstemmed | Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues |
| title_short | Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues |
| title_sort | effect of hemodialysis on the biotransformation of oxo eicosatetraenoic acids in peripheral tissues |
| topic | hemodialysis eicosanoids oxo-eicosatetraenoic acid biotransformation arteriovenous difference |
| url | https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20241213 |
| work_keys_str_mv | AT tongliu effectofhemodialysisonthebiotransformationofoxoeicosatetraenoicacidsinperipheraltissues AT gollaschmaik effectofhemodialysisonthebiotransformationofoxoeicosatetraenoicacidsinperipheraltissues AT cluftfriedrich effectofhemodialysisonthebiotransformationofoxoeicosatetraenoicacidsinperipheraltissues AT panlin effectofhemodialysisonthebiotransformationofoxoeicosatetraenoicacidsinperipheraltissues AT junji effectofhemodialysisonthebiotransformationofoxoeicosatetraenoicacidsinperipheraltissues AT yaomeng effectofhemodialysisonthebiotransformationofoxoeicosatetraenoicacidsinperipheraltissues |