Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues

Effect of hemodialysis on the biotransformation of oxo-eicosatetraenoic acids in peripheral tissues

ObjectiveTo analyze the differences of free and esterified oxo-eicosatetraenoic acids (oxo-ETEs) in blood cells and plasma from arterial and venous blood in hemodialysis (HD) patients. MethodsArterial and venous blood samples from 12 patients with end-stage renal disease (ESRD) before and after HD t...

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Bibliographic Details
Main Authors: Tong LIU, Gollasch Maik, C. Luft Friedrich, Pan LIN, Jun JI, Yao MENG
Format: Article
Language:English
Published: Shanghai Chinese Clinical Medicine Press Co., Ltd. 2025-02-01
Series:Zhongguo Linchuang Yixue
Subjects:
hemodialysis
eicosanoids
oxo-eicosatetraenoic acid
biotransformation
arteriovenous difference
Online Access:https://www.c-jcm.com/article/doi/10.12025/j.issn.1008-6358.2025.20241213
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Summary:ObjectiveTo analyze the differences of free and esterified oxo-eicosatetraenoic acids (oxo-ETEs) in blood cells and plasma from arterial and venous blood in hemodialysis (HD) patients. MethodsArterial and venous blood samples from 12 patients with end-stage renal disease (ESRD) before and after HD treatment at Charité – Universitätsmedizin Berlin, Germany, from June to December 2020 were collected. The esterified and free oxo-ETEs derived from arachidonic acid in blood cells and plasma were measured by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). ResultsNeither esterified nor free oxo-ETEs in blood cells displayed significant arteriovenous differences before and after HD. HD predominantly affected the metabolic levels of esterified and free oxo-ETEs in plasma. HD reduced the arteriovenous differences of esterified 12-oxo-ETE, free 15-oxo-ETE, and free 5-oxo-ETE in plasma, while raised the arteriovenous differences of esterified 15-oxo-ETE. ConclusionsThe oxo-ETEs in blood cells are relatively well-stabilized responding to HD treatment, whereas arteriovenous differences of free and esterified oxo-ETEs in plasma are present and active in response to HD treatment, potentially contributing to the cardiovascular disease.
ISSN:1008-6358

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