RNA-Seq Analysis Uncovered Transcriptomic Changes in <i>Poncirus trifoliata</i> Roots Under Long-Term Soil Drought Conditions
Drought is one of the most serious abiotic stresses in citrus plantations. It is thus imperative to fully understand the drought-resistant mechanisms in these plants. Here, RNA-seq was used to analyze the transcriptomic changes in the roots of <i>Poncirus trifoliata</i>, a widely used ro...
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2024-12-01
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| author | Chuncao Song Xingying Zeng Lin Zheng Qin Huang Lingshan Zhong Yong Zhou Hengfu Yin Yanjie Peng |
| author_facet | Chuncao Song Xingying Zeng Lin Zheng Qin Huang Lingshan Zhong Yong Zhou Hengfu Yin Yanjie Peng |
| author_sort | Chuncao Song |
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| description | Drought is one of the most serious abiotic stresses in citrus plantations. It is thus imperative to fully understand the drought-resistant mechanisms in these plants. Here, RNA-seq was used to analyze the transcriptomic changes in the roots of <i>Poncirus trifoliata</i>, a widely used rootstock in citrus plantations, under a 72-day soil drought and a 7-day recovery stage. Our results showed that the genes upregulated under drought were only enriched in the galactose metabolism and protein processing in endoplasmic reticulum pathways. In the galactose metabolism pathway, four genes related to the synthesis of raffinose family oligosaccharides, which act as osmoprotectants and ROS scavengers, were significantly upregulated under long-term drought. Several heat-shock protein (HSP) family genes were significantly upregulated under drought, leading to increased levels of HSPs to alleviate the endoplasmic reticulum-associated degradation of misfolded proteins induced by drought stress. Some other upregulated genes under drought, like late embryogenesis-abundant family genes and lipid transfer protein family genes, might also be crucial to the drought resistance of <i>P. trifoliata</i> roots. <i>MSYJ196370</i> (heat-shock factor family gene) was the top hub gene in the protein–protein interaction analysis of upregulated genes under drought. These findings supplement the transcriptomic response of <i>P. trifoliata</i> root under long-term drought stress. |
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| institution | DOAJ |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-c7db125c6c154ef9a12966b6be0eb35f2025-08-20T02:55:38ZengMDPI AGHorticulturae2311-75242024-12-011012131910.3390/horticulturae10121319RNA-Seq Analysis Uncovered Transcriptomic Changes in <i>Poncirus trifoliata</i> Roots Under Long-Term Soil Drought ConditionsChuncao Song0Xingying Zeng1Lin Zheng2Qin Huang3Lingshan Zhong4Yong Zhou5Hengfu Yin6Yanjie Peng7College of Life Science, Leshan Normal University, Leshan 614000, ChinaCollege of Life Science, Leshan Normal University, Leshan 614000, ChinaCollege of Life Science, Leshan Normal University, Leshan 614000, ChinaCollege of Life Science, Leshan Normal University, Leshan 614000, ChinaCollege of Life Science, Leshan Normal University, Leshan 614000, ChinaLeshan Engineering Technology Research Center for Innovative Utilization of Feature Plant Resources, Leshan 614000, ChinaKey Laboratory of Tree Breeding of Zhejiang Province, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaCollege of Life Science, Leshan Normal University, Leshan 614000, ChinaDrought is one of the most serious abiotic stresses in citrus plantations. It is thus imperative to fully understand the drought-resistant mechanisms in these plants. Here, RNA-seq was used to analyze the transcriptomic changes in the roots of <i>Poncirus trifoliata</i>, a widely used rootstock in citrus plantations, under a 72-day soil drought and a 7-day recovery stage. Our results showed that the genes upregulated under drought were only enriched in the galactose metabolism and protein processing in endoplasmic reticulum pathways. In the galactose metabolism pathway, four genes related to the synthesis of raffinose family oligosaccharides, which act as osmoprotectants and ROS scavengers, were significantly upregulated under long-term drought. Several heat-shock protein (HSP) family genes were significantly upregulated under drought, leading to increased levels of HSPs to alleviate the endoplasmic reticulum-associated degradation of misfolded proteins induced by drought stress. Some other upregulated genes under drought, like late embryogenesis-abundant family genes and lipid transfer protein family genes, might also be crucial to the drought resistance of <i>P. trifoliata</i> roots. <i>MSYJ196370</i> (heat-shock factor family gene) was the top hub gene in the protein–protein interaction analysis of upregulated genes under drought. These findings supplement the transcriptomic response of <i>P. trifoliata</i> root under long-term drought stress.https://www.mdpi.com/2311-7524/10/12/1319<i>Poncirus trifoliata</i>long-term droughtRNA-seqHSPraffinose family oligosaccharides |
| spellingShingle | Chuncao Song Xingying Zeng Lin Zheng Qin Huang Lingshan Zhong Yong Zhou Hengfu Yin Yanjie Peng RNA-Seq Analysis Uncovered Transcriptomic Changes in <i>Poncirus trifoliata</i> Roots Under Long-Term Soil Drought Conditions Horticulturae <i>Poncirus trifoliata</i> long-term drought RNA-seq HSP raffinose family oligosaccharides |
| title | RNA-Seq Analysis Uncovered Transcriptomic Changes in <i>Poncirus trifoliata</i> Roots Under Long-Term Soil Drought Conditions |
| title_full | RNA-Seq Analysis Uncovered Transcriptomic Changes in <i>Poncirus trifoliata</i> Roots Under Long-Term Soil Drought Conditions |
| title_fullStr | RNA-Seq Analysis Uncovered Transcriptomic Changes in <i>Poncirus trifoliata</i> Roots Under Long-Term Soil Drought Conditions |
| title_full_unstemmed | RNA-Seq Analysis Uncovered Transcriptomic Changes in <i>Poncirus trifoliata</i> Roots Under Long-Term Soil Drought Conditions |
| title_short | RNA-Seq Analysis Uncovered Transcriptomic Changes in <i>Poncirus trifoliata</i> Roots Under Long-Term Soil Drought Conditions |
| title_sort | rna seq analysis uncovered transcriptomic changes in i poncirus trifoliata i roots under long term soil drought conditions |
| topic | <i>Poncirus trifoliata</i> long-term drought RNA-seq HSP raffinose family oligosaccharides |
| url | https://www.mdpi.com/2311-7524/10/12/1319 |
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