Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imaging
Thrombosis is the major cause of ischemic stroke and poses a serious health burden globally. Current thrombolytic strategies, such as systematic administration of recombinant human tissue plasminogen activator (rt-PA), are challenged by limited thrombolysis efficiency due to low targeting ability an...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-06-01
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| Series: | Photoacoustics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221359792500045X |
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| author | Mengtao Han Zhiwei Xue Mengchen Yu Nanlin You Yaguang Ren Zhiqiang Xu Zhifeng Wu Yiming He Zonghai Sheng Chengbo Liu Donghai Wang Jingqin Chen |
| author_facet | Mengtao Han Zhiwei Xue Mengchen Yu Nanlin You Yaguang Ren Zhiqiang Xu Zhifeng Wu Yiming He Zonghai Sheng Chengbo Liu Donghai Wang Jingqin Chen |
| author_sort | Mengtao Han |
| collection | DOAJ |
| description | Thrombosis is the major cause of ischemic stroke and poses a serious health burden globally. Current thrombolytic strategies, such as systematic administration of recombinant human tissue plasminogen activator (rt-PA), are challenged by limited thrombolysis efficiency due to low targeting ability and a short plasma half-life. Here, we report a rapid synergistic strategy that integrates sonothrombolysis and rt-PA mediated pharmacological thrombolysis to achieve accurate and efficient treatment of ischemic stroke. The strategy (PLPA@PFP) uses a platelet-biomimetic membrane as a carrier to deliver both perfluoropentane (PFP) and rt-PA, prolonging half-life and effectively accumulating at the thrombus within 0.5 hours. Upon exposure to focused ultrasound, PFP-based cavitation effects significantly enhance thrombus breakdown and rt-PA penetration, enabling synergistic sono/pharmacological thrombolysis both in vitro and in vivo. High-resolution photoacoustic (PA) imaging provides direct assessment of vascular reperfusion following therapeutic intervention in a murine model of ischemic stroke, offering important guidance for clinical treatment. |
| format | Article |
| id | doaj-art-c7d8c0cc175149e9bca5518633f06da8 |
| institution | OA Journals |
| issn | 2213-5979 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj-art-c7d8c0cc175149e9bca5518633f06da82025-08-20T01:55:38ZengElsevierPhotoacoustics2213-59792025-06-014310072210.1016/j.pacs.2025.100722Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imagingMengtao Han0Zhiwei Xue1Mengchen Yu2Nanlin You3Yaguang Ren4Zhiqiang Xu5Zhifeng Wu6Yiming He7Zonghai Sheng8Chengbo Liu9Donghai Wang10Jingqin Chen11Department of Neurosurgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, China; Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; Shandong Key Laboratory of Brain Health and Function Remodeling, Jinan 250012, ChinaDepartment of Neurosurgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, China; Shandong Key Laboratory of Brain Health and Function Remodeling, Jinan 250012, ChinaDepartment of Neurosurgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, China; Shandong Key Laboratory of Brain Health and Function Remodeling, Jinan 250012, ChinaDepartment of Neurosurgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, China; Shandong Key Laboratory of Brain Health and Function Remodeling, Jinan 250012, ChinaResearch Center for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; State Key Laboratory of Biomedical Imaging Science and System, Shenzhen 518055, ChinaResearch Center for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; State Key Laboratory of Biomedical Imaging Science and System, Shenzhen 518055, ChinaResearch Center for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; State Key Laboratory of Biomedical Imaging Science and System, Shenzhen 518055, ChinaDepartment of Neurosurgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, China; Shandong Key Laboratory of Brain Health and Function Remodeling, Jinan 250012, ChinaState Key Laboratory of Biomedical Imaging Science and System, Shenzhen 518055, China; Research Center for Advanced Detection Materials and Medical Imaging Devices, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaResearch Center for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; State Key Laboratory of Biomedical Imaging Science and System, Shenzhen 518055, China; Corresponding authors at: Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.Department of Neurosurgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, China; Shandong Key Laboratory of Brain Health and Function Remodeling, Jinan 250012, China; Department of Neurosurgery, Qilu Hospital of Shandong University Dezhou Hospital, Dezhou 253000, China; Corresponding author at: Department of Neurosurgery, Qilu Hospital of Shandong University, Cheeloo College of Medicine and Institute of Brain and Brain-Inspired Science, Shandong University, Jinan 250012, China.Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China; State Key Laboratory of Biomedical Imaging Science and System, Shenzhen 518055, China; Corresponding authors at: Research Center for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.Thrombosis is the major cause of ischemic stroke and poses a serious health burden globally. Current thrombolytic strategies, such as systematic administration of recombinant human tissue plasminogen activator (rt-PA), are challenged by limited thrombolysis efficiency due to low targeting ability and a short plasma half-life. Here, we report a rapid synergistic strategy that integrates sonothrombolysis and rt-PA mediated pharmacological thrombolysis to achieve accurate and efficient treatment of ischemic stroke. The strategy (PLPA@PFP) uses a platelet-biomimetic membrane as a carrier to deliver both perfluoropentane (PFP) and rt-PA, prolonging half-life and effectively accumulating at the thrombus within 0.5 hours. Upon exposure to focused ultrasound, PFP-based cavitation effects significantly enhance thrombus breakdown and rt-PA penetration, enabling synergistic sono/pharmacological thrombolysis both in vitro and in vivo. High-resolution photoacoustic (PA) imaging provides direct assessment of vascular reperfusion following therapeutic intervention in a murine model of ischemic stroke, offering important guidance for clinical treatment.http://www.sciencedirect.com/science/article/pii/S221359792500045XIschemic strokePhotoacoustic imagingRapid thrombolysisCerebrovascular imagingBiomimetic drug delivery system |
| spellingShingle | Mengtao Han Zhiwei Xue Mengchen Yu Nanlin You Yaguang Ren Zhiqiang Xu Zhifeng Wu Yiming He Zonghai Sheng Chengbo Liu Donghai Wang Jingqin Chen Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imaging Photoacoustics Ischemic stroke Photoacoustic imaging Rapid thrombolysis Cerebrovascular imaging Biomimetic drug delivery system |
| title | Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imaging |
| title_full | Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imaging |
| title_fullStr | Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imaging |
| title_full_unstemmed | Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imaging |
| title_short | Rapid synergistic thrombolysis of ischemic stroke guided by high-resolution and high-speed photoacoustic cerebrovascular imaging |
| title_sort | rapid synergistic thrombolysis of ischemic stroke guided by high resolution and high speed photoacoustic cerebrovascular imaging |
| topic | Ischemic stroke Photoacoustic imaging Rapid thrombolysis Cerebrovascular imaging Biomimetic drug delivery system |
| url | http://www.sciencedirect.com/science/article/pii/S221359792500045X |
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