Electrospun Nanofibrous Membranes for Guided Bone Regeneration: Fabrication, Characterization, and Biocompatibility Evaluation—Toward Smart 2D Biomaterials
Electrospun nanofibrous membranes have gained considerable attention in bone tissue engineering due to their ability to mimic the extracellular matrix and provide a suitable environment for cell attachment and proliferation. This study investigates the fabrication, characterization, and biocompatibi...
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2025-08-01
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| author | Julia Radwan-Pragłowska Aleksandra Kopacz Aleksandra Sierakowska-Byczek Łukasz Janus Piotr Radomski Aleksander Radwan-Pragłowski |
| author_facet | Julia Radwan-Pragłowska Aleksandra Kopacz Aleksandra Sierakowska-Byczek Łukasz Janus Piotr Radomski Aleksander Radwan-Pragłowski |
| author_sort | Julia Radwan-Pragłowska |
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| description | Electrospun nanofibrous membranes have gained considerable attention in bone tissue engineering due to their ability to mimic the extracellular matrix and provide a suitable environment for cell attachment and proliferation. This study investigates the fabrication, characterization, and biocompatibility of poly(L-lactic acid) (PLA)-based membranes enhanced with periclase (MgO) and gold nanoparticles (AuNPs). The membranes were fabricated using an optimized electrospinning process and subsequently characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Fourier-transform infrared spectroscopy (FT-IR), and contact angle measurements. Additionally, in vitro biodegradation studies in simulated body fluid (SBF) and cytocompatibility tests with osteoblast-like cells were conducted. The results demonstrated that the incorporation of MgO and AuNPs significantly influenced the structural and chemical properties of the membranes, improving their wettability and bioactivity. SEM imaging confirmed uniform fiber morphology with well-distributed nanoparticles. FT-IR spectroscopy indicated successful integration of bioactive components into the PLA matrix. Cytocompatibility assays showed that modified membranes promoted higher osteoblast adhesion and proliferation compared to pristine PLA membranes. Furthermore, biodegradation studies revealed a controlled degradation rate suitable for guided bone regeneration applications. These findings suggest that electrospun PLA membranes enriched with MgO and AuNPs present a promising biomaterial for GBR applications, offering improved bioactivity, mechanical stability, and biocompatibility. |
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| id | doaj-art-c7cf0baf4b71406c8d0b25cc38a01f9b |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2025-08-01 |
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| series | Applied Sciences |
| spelling | doaj-art-c7cf0baf4b71406c8d0b25cc38a01f9b2025-08-20T03:36:35ZengMDPI AGApplied Sciences2076-34172025-08-011515871310.3390/app15158713Electrospun Nanofibrous Membranes for Guided Bone Regeneration: Fabrication, Characterization, and Biocompatibility Evaluation—Toward Smart 2D BiomaterialsJulia Radwan-Pragłowska0Aleksandra Kopacz1Aleksandra Sierakowska-Byczek2Łukasz Janus3Piotr Radomski4Aleksander Radwan-Pragłowski5Department of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandDepartment of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandDepartment of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandDepartment of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandDepartment of Chemical Technology and Environmental Analytics, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24 Street, 31-155 Cracow, PolandDepartment of Computer Science, Faculty of Computer Science and Telecommunications, Cracow University of Technology, Warszawska 24, 31-155 Cracow, PolandElectrospun nanofibrous membranes have gained considerable attention in bone tissue engineering due to their ability to mimic the extracellular matrix and provide a suitable environment for cell attachment and proliferation. This study investigates the fabrication, characterization, and biocompatibility of poly(L-lactic acid) (PLA)-based membranes enhanced with periclase (MgO) and gold nanoparticles (AuNPs). The membranes were fabricated using an optimized electrospinning process and subsequently characterized using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), Fourier-transform infrared spectroscopy (FT-IR), and contact angle measurements. Additionally, in vitro biodegradation studies in simulated body fluid (SBF) and cytocompatibility tests with osteoblast-like cells were conducted. The results demonstrated that the incorporation of MgO and AuNPs significantly influenced the structural and chemical properties of the membranes, improving their wettability and bioactivity. SEM imaging confirmed uniform fiber morphology with well-distributed nanoparticles. FT-IR spectroscopy indicated successful integration of bioactive components into the PLA matrix. Cytocompatibility assays showed that modified membranes promoted higher osteoblast adhesion and proliferation compared to pristine PLA membranes. Furthermore, biodegradation studies revealed a controlled degradation rate suitable for guided bone regeneration applications. These findings suggest that electrospun PLA membranes enriched with MgO and AuNPs present a promising biomaterial for GBR applications, offering improved bioactivity, mechanical stability, and biocompatibility.https://www.mdpi.com/2076-3417/15/15/8713compositesbiomaterialsnanoparticlesguided bone regeneration |
| spellingShingle | Julia Radwan-Pragłowska Aleksandra Kopacz Aleksandra Sierakowska-Byczek Łukasz Janus Piotr Radomski Aleksander Radwan-Pragłowski Electrospun Nanofibrous Membranes for Guided Bone Regeneration: Fabrication, Characterization, and Biocompatibility Evaluation—Toward Smart 2D Biomaterials Applied Sciences composites biomaterials nanoparticles guided bone regeneration |
| title | Electrospun Nanofibrous Membranes for Guided Bone Regeneration: Fabrication, Characterization, and Biocompatibility Evaluation—Toward Smart 2D Biomaterials |
| title_full | Electrospun Nanofibrous Membranes for Guided Bone Regeneration: Fabrication, Characterization, and Biocompatibility Evaluation—Toward Smart 2D Biomaterials |
| title_fullStr | Electrospun Nanofibrous Membranes for Guided Bone Regeneration: Fabrication, Characterization, and Biocompatibility Evaluation—Toward Smart 2D Biomaterials |
| title_full_unstemmed | Electrospun Nanofibrous Membranes for Guided Bone Regeneration: Fabrication, Characterization, and Biocompatibility Evaluation—Toward Smart 2D Biomaterials |
| title_short | Electrospun Nanofibrous Membranes for Guided Bone Regeneration: Fabrication, Characterization, and Biocompatibility Evaluation—Toward Smart 2D Biomaterials |
| title_sort | electrospun nanofibrous membranes for guided bone regeneration fabrication characterization and biocompatibility evaluation toward smart 2d biomaterials |
| topic | composites biomaterials nanoparticles guided bone regeneration |
| url | https://www.mdpi.com/2076-3417/15/15/8713 |
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