Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats
Background: Hyperglycemia is characterized by elevated blood glucose levels caused by impaired insulin production or function, leading to oxidative stress. Probiotic-fermented milk containing Lactiplantibacillus pentosus strain HBUAS 53657 (PFM) has antioxidant and antimicrobial properties that may...
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Indonesian Society for Biochemistry and Molecular Biology
2024-12-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/174 |
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| author | Rita Yaswir Eti Yerizel Netti Suharti Cimi Ilmiawati |
| author_facet | Rita Yaswir Eti Yerizel Netti Suharti Cimi Ilmiawati |
| author_sort | Rita Yaswir |
| collection | DOAJ |
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Background: Hyperglycemia is characterized by elevated blood glucose levels caused by impaired insulin production or function, leading to oxidative stress. Probiotic-fermented milk containing Lactiplantibacillus pentosus strain HBUAS 53657 (PFM) has antioxidant and antimicrobial properties that may reduce oxidative stress.
Objective: This study aimed to investigate the effects of PFM L. pentosus strain HBUAS 53657 on serum glutathione peroxidase (GPx) activity and pancreatic histopathology in hyperglycemic rats.
Method: A post-test-only experimental design was conducted using 25 adult male Wistar rats divided into five groups (n = 5 per group): normal control, hyperglycemic control group, and hyperglycemic rats treated with PFM at doses of 1 × 10⁸ CFU/day (P1), 1 × 10⁹ CFU/day (P2), and 1 × 10¹⁰ CFU/day (P3) for 28 days. Serum GPx activity was assessed via spectrophotometry, Langerhans islet area was measured using ImageJ, and islet damage was evaluated using the Ningrum Score. Statistical analysis was performed using One-Way ANOVA and Kruskal-Wallis tests.
Results: PFM administration at all doses significantly increased serum GPx activity (p = 0.002), with an optimal dose of 1 × 10⁹ CFU/day (P2). PFM also increased the Langerhans islet area (p = 0.001) and reduced islet damage scores (p < 0.001) in a dose-dependent manner.
Conclusion: Probiotic-fermented milk containing Lactiplantibacillus pentosus strain HBUAS 53657 increases serum GPx activity, expands Langerhans islet area, and reduces islet damage in hyperglycemic rats.
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| format | Article |
| id | doaj-art-c7caf446e3844a048db9d2eb303b3a25 |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-c7caf446e3844a048db9d2eb303b3a252025-02-08T03:04:40ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222024-12-017210.32889/actabioina.174Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic ratsRita Yaswir0Eti Yerizel1Netti Suharti2Cimi Ilmiawati3Master Program of Biomedical Science, Faculty of Medicine, Universitas Andalas, Padang 25127, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Universitas Andalas, Padang 25127, Indonesia Department of Biomedical Science, Faculty of Medicine, Universitas Andalas, Padang 25127, IndonesiaDepartement of Pharmacology, Faculty of Medicine, Universitas Andalas, Padang 25127, Indonesia Background: Hyperglycemia is characterized by elevated blood glucose levels caused by impaired insulin production or function, leading to oxidative stress. Probiotic-fermented milk containing Lactiplantibacillus pentosus strain HBUAS 53657 (PFM) has antioxidant and antimicrobial properties that may reduce oxidative stress. Objective: This study aimed to investigate the effects of PFM L. pentosus strain HBUAS 53657 on serum glutathione peroxidase (GPx) activity and pancreatic histopathology in hyperglycemic rats. Method: A post-test-only experimental design was conducted using 25 adult male Wistar rats divided into five groups (n = 5 per group): normal control, hyperglycemic control group, and hyperglycemic rats treated with PFM at doses of 1 × 10⁸ CFU/day (P1), 1 × 10⁹ CFU/day (P2), and 1 × 10¹⁰ CFU/day (P3) for 28 days. Serum GPx activity was assessed via spectrophotometry, Langerhans islet area was measured using ImageJ, and islet damage was evaluated using the Ningrum Score. Statistical analysis was performed using One-Way ANOVA and Kruskal-Wallis tests. Results: PFM administration at all doses significantly increased serum GPx activity (p = 0.002), with an optimal dose of 1 × 10⁹ CFU/day (P2). PFM also increased the Langerhans islet area (p = 0.001) and reduced islet damage scores (p < 0.001) in a dose-dependent manner. Conclusion: Probiotic-fermented milk containing Lactiplantibacillus pentosus strain HBUAS 53657 increases serum GPx activity, expands Langerhans islet area, and reduces islet damage in hyperglycemic rats. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/174antioxidantglutathione peroxidasehyperglycemiapancreatic histopathologyprobiotics |
| spellingShingle | Rita Yaswir Eti Yerizel Netti Suharti Cimi Ilmiawati Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats Acta Biochimica Indonesiana antioxidant glutathione peroxidase hyperglycemia pancreatic histopathology probiotics |
| title | Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats |
| title_full | Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats |
| title_fullStr | Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats |
| title_full_unstemmed | Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats |
| title_short | Effect of probiotic-fermented milk containing Lactiplantibacillus pentoses strain HBUAS 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats |
| title_sort | effect of probiotic fermented milk containing lactiplantibacillus pentoses strain hbuas 53657 on serum glutathione peroxidase activity and pancreatic histopathology in hyperglycemic rats |
| topic | antioxidant glutathione peroxidase hyperglycemia pancreatic histopathology probiotics |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/174 |
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