Investigation of the repairing effects and mechanisms of Polygonatum Odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical-induced oxidative stress

This study established a model system consisting of yak myofibrillar protein (MP) and the Fenton system to investigate the effects of different concentrations of Polygonatum Odoratum Polysaccharides (POP) (0.0 %, 0.2 %, 0.5 %, 1.0 %, 2.0 %) on the physicochemical properties and gel behavior of MP in...

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Main Authors: Xinying Wang, Rongsheng Du, Yuan Liu, Linlin Wang, Lina Wang
Format: Article
Language:English
Published: Elsevier 2025-05-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525004201
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author Xinying Wang
Rongsheng Du
Yuan Liu
Linlin Wang
Lina Wang
author_facet Xinying Wang
Rongsheng Du
Yuan Liu
Linlin Wang
Lina Wang
author_sort Xinying Wang
collection DOAJ
description This study established a model system consisting of yak myofibrillar protein (MP) and the Fenton system to investigate the effects of different concentrations of Polygonatum Odoratum Polysaccharides (POP) (0.0 %, 0.2 %, 0.5 %, 1.0 %, 2.0 %) on the physicochemical properties and gel behavior of MP induced by hydroxyl radicals (·OH). The results indicated that POP inhibited the oxidation-induced deterioration of protein structure, enhancing the integrity and stability of the MP spatial structure and restoring its gel properties. POP suppressed the ·OH attack on MP's amino acid side chains, reducing oxidation-induced protein crosslinking and aggregation. Moreover, it was observed that the protective effect of POP on MP against oxidative damage was concentration-dependent, with the 2.0 % concentration exhibiting excellent antioxidant properties. Overall, these findings suggest that the addition of POP alleviated the oxidative damage caused by ·OH oxidation on MP and promoted the formation of enhanced gel characteristics.
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issn 2590-1575
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publishDate 2025-05-01
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series Food Chemistry: X
spelling doaj-art-c7c6bf87eec5435d85969f2cbef79e132025-08-20T03:22:23ZengElsevierFood Chemistry: X2590-15752025-05-012810257310.1016/j.fochx.2025.102573Investigation of the repairing effects and mechanisms of Polygonatum Odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical-induced oxidative stressXinying Wang0Rongsheng Du1Yuan Liu2Linlin Wang3Lina Wang4College of Pharmacy and Food, Southwest Minzu University, Chengdu, Sichuan 610041, ChinaSichuan Institute for Drug Control, Chengdu, Sichuan 611731, ChinaCollege of Grassland Resources, Southwest Minzu University, Chengdu, Sichuan 610041, ChinaCollege of Pharmacy and Food, Southwest Minzu University, Chengdu, Sichuan 610041, China; Corresponding authors.College of Pharmacy and Food, Southwest Minzu University, Chengdu, Sichuan 610041, China; Corresponding authors.This study established a model system consisting of yak myofibrillar protein (MP) and the Fenton system to investigate the effects of different concentrations of Polygonatum Odoratum Polysaccharides (POP) (0.0 %, 0.2 %, 0.5 %, 1.0 %, 2.0 %) on the physicochemical properties and gel behavior of MP induced by hydroxyl radicals (·OH). The results indicated that POP inhibited the oxidation-induced deterioration of protein structure, enhancing the integrity and stability of the MP spatial structure and restoring its gel properties. POP suppressed the ·OH attack on MP's amino acid side chains, reducing oxidation-induced protein crosslinking and aggregation. Moreover, it was observed that the protective effect of POP on MP against oxidative damage was concentration-dependent, with the 2.0 % concentration exhibiting excellent antioxidant properties. Overall, these findings suggest that the addition of POP alleviated the oxidative damage caused by ·OH oxidation on MP and promoted the formation of enhanced gel characteristics.http://www.sciencedirect.com/science/article/pii/S2590157525004201Polygonatum Odoratum polysaccharidesFenton systemAntioxidantYak myofibrillar proteinPhysicochemical propertiesGelation
spellingShingle Xinying Wang
Rongsheng Du
Yuan Liu
Linlin Wang
Lina Wang
Investigation of the repairing effects and mechanisms of Polygonatum Odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical-induced oxidative stress
Food Chemistry: X
Polygonatum Odoratum polysaccharides
Fenton system
Antioxidant
Yak myofibrillar protein
Physicochemical properties
Gelation
title Investigation of the repairing effects and mechanisms of Polygonatum Odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical-induced oxidative stress
title_full Investigation of the repairing effects and mechanisms of Polygonatum Odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical-induced oxidative stress
title_fullStr Investigation of the repairing effects and mechanisms of Polygonatum Odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical-induced oxidative stress
title_full_unstemmed Investigation of the repairing effects and mechanisms of Polygonatum Odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical-induced oxidative stress
title_short Investigation of the repairing effects and mechanisms of Polygonatum Odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical-induced oxidative stress
title_sort investigation of the repairing effects and mechanisms of polygonatum odoratum polysaccharides on myofibrillar protein gelation under hydroxyl radical induced oxidative stress
topic Polygonatum Odoratum polysaccharides
Fenton system
Antioxidant
Yak myofibrillar protein
Physicochemical properties
Gelation
url http://www.sciencedirect.com/science/article/pii/S2590157525004201
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