Yeast-fermented feed improves high-concentrate diet-induced mastitis in dairy goats by regulating rumen microbiota

Mastitis is a crucial disease that restricts the development of the dairy industry. In production practice, long-term high-concentrate diet (HCD) is often employed to boost milk yield. However, this can lead to rumen microbiota disorder and eventually results in mastitis. Microbial fermented feed ha...

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Main Authors: Keyi Wu, Xiaochun Sun, Jiawen Xu, Zhihang Guan, Weijie Yuan, Lijuan Bao, Yihong Zhao, Ruping Shan, Hui Chen, Caijun Zhao, Xiaoyu Hu, Yunhe Fu, Dacheng Liu, Naisheng Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Microbiology
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Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1582314/full
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author Keyi Wu
Keyi Wu
Xiaochun Sun
Jiawen Xu
Zhihang Guan
Weijie Yuan
Lijuan Bao
Yihong Zhao
Ruping Shan
Hui Chen
Caijun Zhao
Xiaoyu Hu
Yunhe Fu
Dacheng Liu
Naisheng Zhang
author_facet Keyi Wu
Keyi Wu
Xiaochun Sun
Jiawen Xu
Zhihang Guan
Weijie Yuan
Lijuan Bao
Yihong Zhao
Ruping Shan
Hui Chen
Caijun Zhao
Xiaoyu Hu
Yunhe Fu
Dacheng Liu
Naisheng Zhang
author_sort Keyi Wu
collection DOAJ
description Mastitis is a crucial disease that restricts the development of the dairy industry. In production practice, long-term high-concentrate diet (HCD) is often employed to boost milk yield. However, this can lead to rumen microbiota disorder and eventually results in mastitis. Microbial fermented feed has drawn increasing attention due to its abundant functions, safety, and effectiveness. Yeast, as a widely used fungus, has excellent fermentation performance and a variety of beneficial physiological functions. In this study, we investigated the therapeutic effect of yeast fermented feed (YFF) on mastitis in dairy goats induced by ruminal dysbiosis. Twenty-four dairy goats in late lactation, with an initial body weight of 34.65 ± 5.46 kg, were selected for the study. The total experimental period lasted 55 days. In the control group, a roughage-to-concentrate ratio (F:C) of 7:3 was consistently provided. For the subacute ruminal acidosis (SARA) group, eight dairy goats were given a 5:5 F:C diet free of charge for 10 days prior to the start of the experiment to ensure their adaptation to the diet. Subsequently, these eight dairy goats were fed an HCD consisting of 30% roughage and 70% mixed concentrate until the end of the experiment. For the SARA+Y group, eight dairy goats were fed the same diet as the SARA group from the beginning of the experiment until day 45. After that, 80 to 100 grams of YFF per goat were added to the diet daily for 10 days until the end of the experiment. In this study, adding YFF on the basis of HCD alleviated mastitis by restoring the function of the rumen barrier and regulating the imbalance of rumen microbiota. It alleviated the symptoms of SARA, reduced the levels of LPS in the rumen, serum, and mammary glands, and reduced the levels of proinflammatory cytokines. Specifically, it was reflected in restoring the function of the blood-milk barrier, limiting the inflammatory response, and reducing oxidative stress. In conclusion, these results suggest that supplementation with YFF alleviates mastitis induced by ruminal microbiota disturbance due to feeding HCD in several ways. This finding paves the way for a new approach and method to address mastitis in ruminant animals. It not only helps enhance the health level of ruminants but also plays a positive role in improving breeding efficiency. At the same time, it provides a strong guarantee for enhancing the quality of dairy products and lays a solid foundation for the sustainable development of the dairy industry.
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publishDate 2025-06-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Microbiology
spelling doaj-art-c7c5025d67b64a36a0c06bf3fbe960e42025-08-20T03:27:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-06-011610.3389/fmicb.2025.15823141582314Yeast-fermented feed improves high-concentrate diet-induced mastitis in dairy goats by regulating rumen microbiotaKeyi Wu0Keyi Wu1Xiaochun Sun2Jiawen Xu3Zhihang Guan4Weijie Yuan5Lijuan Bao6Yihong Zhao7Ruping Shan8Hui Chen9Caijun Zhao10Xiaoyu Hu11Yunhe Fu12Dacheng Liu13Naisheng Zhang14Department of Gynecology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaDepartment of Gynecology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, ChinaDepartment of Clinical Veterinary Medicine, College of Veterinary Medicine, Jilin University, Changchun, Jilin, ChinaMastitis is a crucial disease that restricts the development of the dairy industry. In production practice, long-term high-concentrate diet (HCD) is often employed to boost milk yield. However, this can lead to rumen microbiota disorder and eventually results in mastitis. Microbial fermented feed has drawn increasing attention due to its abundant functions, safety, and effectiveness. Yeast, as a widely used fungus, has excellent fermentation performance and a variety of beneficial physiological functions. In this study, we investigated the therapeutic effect of yeast fermented feed (YFF) on mastitis in dairy goats induced by ruminal dysbiosis. Twenty-four dairy goats in late lactation, with an initial body weight of 34.65 ± 5.46 kg, were selected for the study. The total experimental period lasted 55 days. In the control group, a roughage-to-concentrate ratio (F:C) of 7:3 was consistently provided. For the subacute ruminal acidosis (SARA) group, eight dairy goats were given a 5:5 F:C diet free of charge for 10 days prior to the start of the experiment to ensure their adaptation to the diet. Subsequently, these eight dairy goats were fed an HCD consisting of 30% roughage and 70% mixed concentrate until the end of the experiment. For the SARA+Y group, eight dairy goats were fed the same diet as the SARA group from the beginning of the experiment until day 45. After that, 80 to 100 grams of YFF per goat were added to the diet daily for 10 days until the end of the experiment. In this study, adding YFF on the basis of HCD alleviated mastitis by restoring the function of the rumen barrier and regulating the imbalance of rumen microbiota. It alleviated the symptoms of SARA, reduced the levels of LPS in the rumen, serum, and mammary glands, and reduced the levels of proinflammatory cytokines. Specifically, it was reflected in restoring the function of the blood-milk barrier, limiting the inflammatory response, and reducing oxidative stress. In conclusion, these results suggest that supplementation with YFF alleviates mastitis induced by ruminal microbiota disturbance due to feeding HCD in several ways. This finding paves the way for a new approach and method to address mastitis in ruminant animals. It not only helps enhance the health level of ruminants but also plays a positive role in improving breeding efficiency. At the same time, it provides a strong guarantee for enhancing the quality of dairy products and lays a solid foundation for the sustainable development of the dairy industry.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1582314/fullyeast-fermented feedmastitisrumen microbiotahigh concentrate dietLPS
spellingShingle Keyi Wu
Keyi Wu
Xiaochun Sun
Jiawen Xu
Zhihang Guan
Weijie Yuan
Lijuan Bao
Yihong Zhao
Ruping Shan
Hui Chen
Caijun Zhao
Xiaoyu Hu
Yunhe Fu
Dacheng Liu
Naisheng Zhang
Yeast-fermented feed improves high-concentrate diet-induced mastitis in dairy goats by regulating rumen microbiota
Frontiers in Microbiology
yeast-fermented feed
mastitis
rumen microbiota
high concentrate diet
LPS
title Yeast-fermented feed improves high-concentrate diet-induced mastitis in dairy goats by regulating rumen microbiota
title_full Yeast-fermented feed improves high-concentrate diet-induced mastitis in dairy goats by regulating rumen microbiota
title_fullStr Yeast-fermented feed improves high-concentrate diet-induced mastitis in dairy goats by regulating rumen microbiota
title_full_unstemmed Yeast-fermented feed improves high-concentrate diet-induced mastitis in dairy goats by regulating rumen microbiota
title_short Yeast-fermented feed improves high-concentrate diet-induced mastitis in dairy goats by regulating rumen microbiota
title_sort yeast fermented feed improves high concentrate diet induced mastitis in dairy goats by regulating rumen microbiota
topic yeast-fermented feed
mastitis
rumen microbiota
high concentrate diet
LPS
url https://www.frontiersin.org/articles/10.3389/fmicb.2025.1582314/full
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