Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury

The hydroalcoholic extract of Punica granatum (pomegranate) leaves was previously demonstrated to be anti-inflammatory in a rat model of lipopolysaccharide- (LPS-) induced acute peritonitis. Here, we investigated the anti-inflammatory effects of the ethyl acetate fraction obtained from the pomegrana...

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Main Authors: Aruanã Joaquim Matheus Costa Rodrigues Pinheiro, Jaciara Sá Gonçalves, Ádylla Wilenna Alves Dourado, Eduardo Martins de Sousa, Natilene Mesquita Brito, Lanna Karinny Silva, Marisa Cristina Aranha Batista, Joicy Cortez de Sá, Cinara Regina Aragão Vieira Monteiro, Elizabeth Soares Fernandes, Valério Monteiro-Neto, Lee Ann Campbell, Patrícia Maria Wiziack Zago, Lidio Gonçalves Lima-Neto
Format: Article
Language:English
Published: Wiley 2018-01-01
Series:Journal of Immunology Research
Online Access:http://dx.doi.org/10.1155/2018/6879183
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author Aruanã Joaquim Matheus Costa Rodrigues Pinheiro
Jaciara Sá Gonçalves
Ádylla Wilenna Alves Dourado
Eduardo Martins de Sousa
Natilene Mesquita Brito
Lanna Karinny Silva
Marisa Cristina Aranha Batista
Joicy Cortez de Sá
Cinara Regina Aragão Vieira Monteiro
Elizabeth Soares Fernandes
Valério Monteiro-Neto
Lee Ann Campbell
Patrícia Maria Wiziack Zago
Lidio Gonçalves Lima-Neto
author_facet Aruanã Joaquim Matheus Costa Rodrigues Pinheiro
Jaciara Sá Gonçalves
Ádylla Wilenna Alves Dourado
Eduardo Martins de Sousa
Natilene Mesquita Brito
Lanna Karinny Silva
Marisa Cristina Aranha Batista
Joicy Cortez de Sá
Cinara Regina Aragão Vieira Monteiro
Elizabeth Soares Fernandes
Valério Monteiro-Neto
Lee Ann Campbell
Patrícia Maria Wiziack Zago
Lidio Gonçalves Lima-Neto
author_sort Aruanã Joaquim Matheus Costa Rodrigues Pinheiro
collection DOAJ
description The hydroalcoholic extract of Punica granatum (pomegranate) leaves was previously demonstrated to be anti-inflammatory in a rat model of lipopolysaccharide- (LPS-) induced acute peritonitis. Here, we investigated the anti-inflammatory effects of the ethyl acetate fraction obtained from the pomegranate leaf hydroalcoholic extract (EAFPg) on the LPS-induced acute lung injury (ALI) mouse model. Male Swiss mice received either EAFPg at different doses or dexamethasone (per os) prior to LPS intranasal instillation. Vehicle-treated mice were used as controls. Animals were culled at 4 h after LPS challenge, and the bronchoalveolar lavage fluid (BALF) and lung samples were collected for analysis. EAFPg and kaempferol effects on NO and cytokine production by LPS-stimulated RAW 264.7 macrophages were also investigated. Pretreatment with EAFPg (100–300 mg/kg) markedly reduced cell accumulation (specially neutrophils) and collagen deposition in the lungs of ALI mice. The same animals presented with reduced lung and BALF TNF-α and IL-1β expression in comparison with vehicle controls (p<0.05). Additionally, incubation with either EAFPg or kaempferol (100 μg/ml) reduced NO production and cytokine gene expression in cultured LPS-treated RAW 264.7 macrophages. Overall, these results demonstrate that the prophylactic treatment with EAFPg attenuates acute lung inflammation. We suggest this fraction may be useful in treating ALI.
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spelling doaj-art-c7c4b0ccc1d6418ca8f19731d013e8fb2025-08-20T03:24:11ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/68791836879183Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung InjuryAruanã Joaquim Matheus Costa Rodrigues Pinheiro0Jaciara Sá Gonçalves1Ádylla Wilenna Alves Dourado2Eduardo Martins de Sousa3Natilene Mesquita Brito4Lanna Karinny Silva5Marisa Cristina Aranha Batista6Joicy Cortez de Sá7Cinara Regina Aragão Vieira Monteiro8Elizabeth Soares Fernandes9Valério Monteiro-Neto10Lee Ann Campbell11Patrícia Maria Wiziack Zago12Lidio Gonçalves Lima-Neto13Programa de Pós-Graduação, Universidade CEUMA, São Luís, MA, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís, MA, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís, MA, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís, MA, BrazilLaboratório de Estudos Ambientais, Instituto Federal do Maranhão, São Luís, MA, BrazilLaboratório de Estudos Ambientais, Instituto Federal do Maranhão, São Luís, MA, BrazilLaboratório de Farmacognosia I, Universidade Federal do Maranhão, São Luís, MA, BrazilDepartamento de Medicina, Universidade CEUMA, São Luís, MA, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís, MA, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís, MA, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís, MA, BrazilDepartment of Global Health, University of Washington, Seattle, WA, USADepartamento de Odontologia, Universidade CEUMA, São Luís, MA, BrazilPrograma de Pós-Graduação, Universidade CEUMA, São Luís, MA, BrazilThe hydroalcoholic extract of Punica granatum (pomegranate) leaves was previously demonstrated to be anti-inflammatory in a rat model of lipopolysaccharide- (LPS-) induced acute peritonitis. Here, we investigated the anti-inflammatory effects of the ethyl acetate fraction obtained from the pomegranate leaf hydroalcoholic extract (EAFPg) on the LPS-induced acute lung injury (ALI) mouse model. Male Swiss mice received either EAFPg at different doses or dexamethasone (per os) prior to LPS intranasal instillation. Vehicle-treated mice were used as controls. Animals were culled at 4 h after LPS challenge, and the bronchoalveolar lavage fluid (BALF) and lung samples were collected for analysis. EAFPg and kaempferol effects on NO and cytokine production by LPS-stimulated RAW 264.7 macrophages were also investigated. Pretreatment with EAFPg (100–300 mg/kg) markedly reduced cell accumulation (specially neutrophils) and collagen deposition in the lungs of ALI mice. The same animals presented with reduced lung and BALF TNF-α and IL-1β expression in comparison with vehicle controls (p<0.05). Additionally, incubation with either EAFPg or kaempferol (100 μg/ml) reduced NO production and cytokine gene expression in cultured LPS-treated RAW 264.7 macrophages. Overall, these results demonstrate that the prophylactic treatment with EAFPg attenuates acute lung inflammation. We suggest this fraction may be useful in treating ALI.http://dx.doi.org/10.1155/2018/6879183
spellingShingle Aruanã Joaquim Matheus Costa Rodrigues Pinheiro
Jaciara Sá Gonçalves
Ádylla Wilenna Alves Dourado
Eduardo Martins de Sousa
Natilene Mesquita Brito
Lanna Karinny Silva
Marisa Cristina Aranha Batista
Joicy Cortez de Sá
Cinara Regina Aragão Vieira Monteiro
Elizabeth Soares Fernandes
Valério Monteiro-Neto
Lee Ann Campbell
Patrícia Maria Wiziack Zago
Lidio Gonçalves Lima-Neto
Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury
Journal of Immunology Research
title Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury
title_full Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury
title_fullStr Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury
title_full_unstemmed Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury
title_short Punica granatum L. Leaf Extract Attenuates Lung Inflammation in Mice with Acute Lung Injury
title_sort punica granatum l leaf extract attenuates lung inflammation in mice with acute lung injury
url http://dx.doi.org/10.1155/2018/6879183
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