Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity

Biomaterials with antimicrobial properties are a key research area due to the increasing threat of infections and the growing resistance of microorganisms to existing antibiotics. The aim of the study was to produce thermally cross-linked polymer films based on poly(1,5-pentanediol azelate) and poly...

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Main Authors: Aleksandra Bandzerewicz, Anna Herman, Ewa Dutkowska, Klara Niebuda, Paweł Ruśkowski, Agnieszka Gadomska-Gajadhur
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Bioengineering and Biotechnology
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Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2025.1579630/full
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author Aleksandra Bandzerewicz
Anna Herman
Ewa Dutkowska
Klara Niebuda
Paweł Ruśkowski
Agnieszka Gadomska-Gajadhur
author_facet Aleksandra Bandzerewicz
Anna Herman
Ewa Dutkowska
Klara Niebuda
Paweł Ruśkowski
Agnieszka Gadomska-Gajadhur
author_sort Aleksandra Bandzerewicz
collection DOAJ
description Biomaterials with antimicrobial properties are a key research area due to the increasing threat of infections and the growing resistance of microorganisms to existing antibiotics. The aim of the study was to produce thermally cross-linked polymer films based on poly(1,5-pentanediol azelate) and poly(1,4-butanediol citrate) with antimicrobial activity for medical applications. Well-formed, cross-linked, flexible materials differing in appearance depending on the conditions of the cross-linking process were obtained. In general, a lower cross-linking temperature was found to promote less brittle and more flexible films with greater structure uniformity. The polymer films had hydrophilic surfaces (water contact angle 40°–60°). All polymer films maintained integrity after immersion in PBS buffer. Most likely, the lower hydrophilicity of the polyazelate phase limited their degradation. A modified time-kill procedure (ASTM E2315-23) was performed to test the antimicrobial properties of the films against Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans. The antimicrobial activity of polycitrate-based films against P. aeruginosa has been reported with >90% reduction of the pathogen after 6 h of contact and 100% biocidal effect after 24 h. The antimicrobial activity of the film is pH-based. The biocidal effect of polycitrate film against P. aeruginosa is the most important and promising result, especially given the resistance of the pathogen to commonly used antibiotics.
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spelling doaj-art-c7c052ffada04265aba4380a1dbc968d2025-08-20T02:23:00ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-06-011310.3389/fbioe.2025.15796301579630Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activityAleksandra BandzerewiczAnna HermanEwa DutkowskaKlara NiebudaPaweł RuśkowskiAgnieszka Gadomska-GajadhurBiomaterials with antimicrobial properties are a key research area due to the increasing threat of infections and the growing resistance of microorganisms to existing antibiotics. The aim of the study was to produce thermally cross-linked polymer films based on poly(1,5-pentanediol azelate) and poly(1,4-butanediol citrate) with antimicrobial activity for medical applications. Well-formed, cross-linked, flexible materials differing in appearance depending on the conditions of the cross-linking process were obtained. In general, a lower cross-linking temperature was found to promote less brittle and more flexible films with greater structure uniformity. The polymer films had hydrophilic surfaces (water contact angle 40°–60°). All polymer films maintained integrity after immersion in PBS buffer. Most likely, the lower hydrophilicity of the polyazelate phase limited their degradation. A modified time-kill procedure (ASTM E2315-23) was performed to test the antimicrobial properties of the films against Pseudomonas aeruginosa, Staphylococcus aureus, and Candida albicans. The antimicrobial activity of polycitrate-based films against P. aeruginosa has been reported with >90% reduction of the pathogen after 6 h of contact and 100% biocidal effect after 24 h. The antimicrobial activity of the film is pH-based. The biocidal effect of polycitrate film against P. aeruginosa is the most important and promising result, especially given the resistance of the pathogen to commonly used antibiotics.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1579630/fullpolycitratepolyazelatepolymer filmswound dressingsPseudomonas aeruginosaantimicrobial
spellingShingle Aleksandra Bandzerewicz
Anna Herman
Ewa Dutkowska
Klara Niebuda
Paweł Ruśkowski
Agnieszka Gadomska-Gajadhur
Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity
Frontiers in Bioengineering and Biotechnology
polycitrate
polyazelate
polymer films
wound dressings
Pseudomonas aeruginosa
antimicrobial
title Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity
title_full Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity
title_fullStr Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity
title_full_unstemmed Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity
title_short Combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity
title_sort combining polyesters of citric and azelaic acids to obtain potential topical application biomaterials with antimicrobial activity
topic polycitrate
polyazelate
polymer films
wound dressings
Pseudomonas aeruginosa
antimicrobial
url https://www.frontiersin.org/articles/10.3389/fbioe.2025.1579630/full
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