Heat Stress Downregulates Photosystem I Redox State on Leaf Photosynthesis in Grapevine
Semi-arid viticultural regions globally are experiencing severe and frequent growing-season heat waves that negatively impact grapevine (<i>Vitis vinifera</i> L.) physiological performance and productivity. At the leaf level, heat stress can photodamage both Photosystem I (PSI) and Photo...
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MDPI AG
2025-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/15/4/948 |
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| author | Qian Qiu Yanli Sun Dinghan Guo Lei Wang Vinay Pagay Shiping Wang |
| author_facet | Qian Qiu Yanli Sun Dinghan Guo Lei Wang Vinay Pagay Shiping Wang |
| author_sort | Qian Qiu |
| collection | DOAJ |
| description | Semi-arid viticultural regions globally are experiencing severe and frequent growing-season heat waves that negatively impact grapevine (<i>Vitis vinifera</i> L.) physiological performance and productivity. At the leaf level, heat stress can photodamage both Photosystem I (PSI) and Photosystem II (PSII). In order to study the self-protection mechanism of grapevine leaves, in this study, 3-year-old potted ‘Merlot’ and ‘Muscat Hamburg’ grapevines were exposed to a 5-day simulated heatwave (45/25 °C day/night) and compared to vines maintained at 25/18 °C. After heat exposure, ‘Merlot’ demonstrated superior thermotolerance and superior physiological performance as measured by gas exchange, oxidative parameters, chlorophyll loss, and photoinhibition of PSI and PSII. Additionally, non-photochemical quenching (NPQ) dissipated the excess light energy in the form of heat. Y(NPQ) progressively rose from 0 to 0.6, signaling the start of the grapevine leaves’ self-defense against temperature stress. Furthermore, the stimulation of cyclic electron flow (CEF) under high temperatures contributed to the energy balance of PSI. The CEF of ‘Muscat Hamburg’ under high light intensities increased dramatically from 1 to 4. NAD(P)H dehydrogenase-dependent CEF around PSI increased markedly, suggesting its role in self-protection. These results demonstrate that both NPQ and CEF play key photoprotective roles by generating a proton gradient under heat stress. |
| format | Article |
| id | doaj-art-c7bf2ff042ec4003969441759dae5f34 |
| institution | OA Journals |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-c7bf2ff042ec4003969441759dae5f342025-08-20T02:17:19ZengMDPI AGAgronomy2073-43952025-04-0115494810.3390/agronomy15040948Heat Stress Downregulates Photosystem I Redox State on Leaf Photosynthesis in GrapevineQian Qiu0Yanli Sun1Dinghan Guo2Lei Wang3Vinay Pagay4Shiping Wang5Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Agriculture, Food and Wine, The University of Adelaide, PMB 1, Glen Osmond, SA 5064, AustraliaDepartment of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, ChinaSemi-arid viticultural regions globally are experiencing severe and frequent growing-season heat waves that negatively impact grapevine (<i>Vitis vinifera</i> L.) physiological performance and productivity. At the leaf level, heat stress can photodamage both Photosystem I (PSI) and Photosystem II (PSII). In order to study the self-protection mechanism of grapevine leaves, in this study, 3-year-old potted ‘Merlot’ and ‘Muscat Hamburg’ grapevines were exposed to a 5-day simulated heatwave (45/25 °C day/night) and compared to vines maintained at 25/18 °C. After heat exposure, ‘Merlot’ demonstrated superior thermotolerance and superior physiological performance as measured by gas exchange, oxidative parameters, chlorophyll loss, and photoinhibition of PSI and PSII. Additionally, non-photochemical quenching (NPQ) dissipated the excess light energy in the form of heat. Y(NPQ) progressively rose from 0 to 0.6, signaling the start of the grapevine leaves’ self-defense against temperature stress. Furthermore, the stimulation of cyclic electron flow (CEF) under high temperatures contributed to the energy balance of PSI. The CEF of ‘Muscat Hamburg’ under high light intensities increased dramatically from 1 to 4. NAD(P)H dehydrogenase-dependent CEF around PSI increased markedly, suggesting its role in self-protection. These results demonstrate that both NPQ and CEF play key photoprotective roles by generating a proton gradient under heat stress.https://www.mdpi.com/2073-4395/15/4/948grapephotosynthesisextreme heat stressphotosystem Icyclic electron flow |
| spellingShingle | Qian Qiu Yanli Sun Dinghan Guo Lei Wang Vinay Pagay Shiping Wang Heat Stress Downregulates Photosystem I Redox State on Leaf Photosynthesis in Grapevine Agronomy grape photosynthesis extreme heat stress photosystem I cyclic electron flow |
| title | Heat Stress Downregulates Photosystem I Redox State on Leaf Photosynthesis in Grapevine |
| title_full | Heat Stress Downregulates Photosystem I Redox State on Leaf Photosynthesis in Grapevine |
| title_fullStr | Heat Stress Downregulates Photosystem I Redox State on Leaf Photosynthesis in Grapevine |
| title_full_unstemmed | Heat Stress Downregulates Photosystem I Redox State on Leaf Photosynthesis in Grapevine |
| title_short | Heat Stress Downregulates Photosystem I Redox State on Leaf Photosynthesis in Grapevine |
| title_sort | heat stress downregulates photosystem i redox state on leaf photosynthesis in grapevine |
| topic | grape photosynthesis extreme heat stress photosystem I cyclic electron flow |
| url | https://www.mdpi.com/2073-4395/15/4/948 |
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