Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride
Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was pe...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2020/7204951 |
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| author | Carlos Carpena-Torres Jesús Pintor María Jesús Pérez de Lara Fernando Huete-Toral Almudena Crooke Cristina Pastrana Gonzalo Carracedo |
| author_facet | Carlos Carpena-Torres Jesús Pintor María Jesús Pérez de Lara Fernando Huete-Toral Almudena Crooke Cristina Pastrana Gonzalo Carracedo |
| author_sort | Carlos Carpena-Torres |
| collection | DOAJ |
| description | Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. Conclusions. The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days). |
| format | Article |
| id | doaj-art-c7b88590b0ca4deaa6b3e0d35cffb373 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-c7b88590b0ca4deaa6b3e0d35cffb3732025-02-03T01:05:02ZengWileyJournal of Ophthalmology2090-004X2090-00582020-01-01202010.1155/2020/72049517204951Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium ChlorideCarlos Carpena-Torres0Jesús Pintor1María Jesús Pérez de Lara2Fernando Huete-Toral3Almudena Crooke4Cristina Pastrana5Gonzalo Carracedo6Department of Optometry and Vision, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid, SpainDepartment of Optometry and Vision, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid, SpainDepartment of Optometry and Vision, Faculty of Optics and Optometry, Universidad Complutense de Madrid, Madrid, SpainPurpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. Conclusions. The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).http://dx.doi.org/10.1155/2020/7204951 |
| spellingShingle | Carlos Carpena-Torres Jesús Pintor María Jesús Pérez de Lara Fernando Huete-Toral Almudena Crooke Cristina Pastrana Gonzalo Carracedo Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride Journal of Ophthalmology |
| title | Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride |
| title_full | Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride |
| title_fullStr | Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride |
| title_full_unstemmed | Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride |
| title_short | Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride |
| title_sort | optimization of a rabbit dry eye model induced by topical instillation of benzalkonium chloride |
| url | http://dx.doi.org/10.1155/2020/7204951 |
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