Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru
Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new...
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MDPI AG
2024-09-01
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| author | Claudio Quiñones-Cerna Alina Castañeda-Aspajo Marycielo Tirado-Gutierrez David Salirrosas-Fernández Juan Carlos Rodríguez-Soto José Alfredo Cruz-Monzón Fernando Hurtado-Butrón Wilmer Ugarte-López Mayra Gutiérrez-Araujo Medardo Alberto Quezada-Alvarez Julieta Alessandra Gálvez-Rivera Mario Esparza-Mantilla |
| author_facet | Claudio Quiñones-Cerna Alina Castañeda-Aspajo Marycielo Tirado-Gutierrez David Salirrosas-Fernández Juan Carlos Rodríguez-Soto José Alfredo Cruz-Monzón Fernando Hurtado-Butrón Wilmer Ugarte-López Mayra Gutiérrez-Araujo Medardo Alberto Quezada-Alvarez Julieta Alessandra Gálvez-Rivera Mario Esparza-Mantilla |
| author_sort | Claudio Quiñones-Cerna |
| collection | DOAJ |
| description | Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new bacterial strains from underexplored habitats could improve remediation efforts at contaminated sites. This study aimed to evaluate the hydrocarbon biodegradation capacity of bacteria isolated from agricultural soils in Huamachuco, Peru. Soil samples from Oca crops were collected and bacteria were isolated. Biodegradation assays were conducted using diesel as the sole carbon source in the Bushnell Haas Mineral medium. Molecular characterization of the 16S rRNA gene identified four strains. Diesel biodegradation assays at 1% concentration were performed under agitation conditions at 150 rpm and 30 °C, and monitored on day 10 by measuring cellular biomass (OD<sub>600</sub>), with hydrocarbons analyzed by gas chromatography. The results showed <i>Pseudomonas protegens</i> (PROM2) achieved the highest efficiency in removing total hydrocarbons (91.5 ± 0.7%). Additionally, <i>Pseudomonas citri</i> PROM3 and <i>Acinetobacter guillouiae</i> ClyRoM5 also demonstrated high capacity in removing several individual hydrocarbons. Indigenous bacteria from uncontaminated agricultural soils present a high potential for hydrocarbon bioremediation, offering an ecological and effective solution for soil decontamination. |
| format | Article |
| id | doaj-art-c7b65f7bdec3467f9dae293adad84673 |
| institution | OA Journals |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-c7b65f7bdec3467f9dae293adad846732025-08-20T01:55:41ZengMDPI AGMicroorganisms2076-26072024-09-01129189610.3390/microorganisms12091896Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, PeruClaudio Quiñones-Cerna0Alina Castañeda-Aspajo1Marycielo Tirado-Gutierrez2David Salirrosas-Fernández3Juan Carlos Rodríguez-Soto4José Alfredo Cruz-Monzón5Fernando Hurtado-Butrón6Wilmer Ugarte-López7Mayra Gutiérrez-Araujo8Medardo Alberto Quezada-Alvarez9Julieta Alessandra Gálvez-Rivera10Mario Esparza-Mantilla11Laboratorio de Biotecnología e Ingeniería Genética, Facultad de Ciencias Biológicas, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruDepartamento de Ingeniería Ambiental, Facultad de Ingeniería Química, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruDepartamento de Ingeniería Ambiental, Facultad de Ingeniería Química, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruLaboratorio de Citometría, Facultad de Ciencias Biológicas, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruLaboratorio de Citometría, Facultad de Ciencias Biológicas, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruDepartamento de Química, Facultad de Ingeniería Química, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruLaboratorio Multidisciplinario de Nanociencia y Nanotecnología “Oswaldo Sánchez Rosales”, Facultad de Ciencias Físicas y Matemáticas, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruDepartamento de Ingeniería Ambiental, Facultad de Ingeniería Química, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruLaboratorio de Citometría, Facultad de Ciencias Biológicas, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruLaboratorio de Investigación y Desarrollo en Ciencias Ambientales, Facultad de Ingeniería Química, Universidad Nacional de Trujillo, Juan Pablo II Av., Trujillo 13008, PeruEscuela Profesional de Ciencias Biológicas, Facultad de Ciencias, Universidad Nacional de Piura, Juan Pablo II Av., Trujillo 13008, PeruFundación Internacional para la Cultura y Ciencias de la Vida, Iquique 1100000, ChilePollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new bacterial strains from underexplored habitats could improve remediation efforts at contaminated sites. This study aimed to evaluate the hydrocarbon biodegradation capacity of bacteria isolated from agricultural soils in Huamachuco, Peru. Soil samples from Oca crops were collected and bacteria were isolated. Biodegradation assays were conducted using diesel as the sole carbon source in the Bushnell Haas Mineral medium. Molecular characterization of the 16S rRNA gene identified four strains. Diesel biodegradation assays at 1% concentration were performed under agitation conditions at 150 rpm and 30 °C, and monitored on day 10 by measuring cellular biomass (OD<sub>600</sub>), with hydrocarbons analyzed by gas chromatography. The results showed <i>Pseudomonas protegens</i> (PROM2) achieved the highest efficiency in removing total hydrocarbons (91.5 ± 0.7%). Additionally, <i>Pseudomonas citri</i> PROM3 and <i>Acinetobacter guillouiae</i> ClyRoM5 also demonstrated high capacity in removing several individual hydrocarbons. Indigenous bacteria from uncontaminated agricultural soils present a high potential for hydrocarbon bioremediation, offering an ecological and effective solution for soil decontamination.https://www.mdpi.com/2076-2607/12/9/1896biotechnologybioremediationmitigationbiodegradationdieselhydrocarbons |
| spellingShingle | Claudio Quiñones-Cerna Alina Castañeda-Aspajo Marycielo Tirado-Gutierrez David Salirrosas-Fernández Juan Carlos Rodríguez-Soto José Alfredo Cruz-Monzón Fernando Hurtado-Butrón Wilmer Ugarte-López Mayra Gutiérrez-Araujo Medardo Alberto Quezada-Alvarez Julieta Alessandra Gálvez-Rivera Mario Esparza-Mantilla Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru Microorganisms biotechnology bioremediation mitigation biodegradation diesel hydrocarbons |
| title | Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru |
| title_full | Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru |
| title_fullStr | Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru |
| title_full_unstemmed | Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru |
| title_short | Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru |
| title_sort | efficacy of indigenous bacteria in the biodegradation of hydrocarbons isolated from agricultural soils in huamachuco peru |
| topic | biotechnology bioremediation mitigation biodegradation diesel hydrocarbons |
| url | https://www.mdpi.com/2076-2607/12/9/1896 |
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