A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice
A high-salt diet (HSD) is common worldwide and can lead to cardiovascular disease, chronic inflammation, and autoimmune diseases. Moreover, increasing evidence shows that HSD is closely related to a variety of immune diseases. Natural killer (NK) cells are important innate immune cells that directly...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/6687143 |
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| author | Xiaokang Zeng Yan Li Weibiao Lv Xinhuai Dong Chong Zeng Liming Zeng Zibo Wei Xu Lin Yanning Ma Qiang Xiao |
| author_facet | Xiaokang Zeng Yan Li Weibiao Lv Xinhuai Dong Chong Zeng Liming Zeng Zibo Wei Xu Lin Yanning Ma Qiang Xiao |
| author_sort | Xiaokang Zeng |
| collection | DOAJ |
| description | A high-salt diet (HSD) is common worldwide and can lead to cardiovascular disease, chronic inflammation, and autoimmune diseases. Moreover, increasing evidence shows that HSD is closely related to a variety of immune diseases. Natural killer (NK) cells are important innate immune cells that directly kill their targets via degranulation and secretion of interferon gamma (IFN-γ). NK cells play a vital role in resisting viruses and preventing the malignant transformation of cells; however, whether HSD affects the development and function of NK cells has not yet been elucidated. Therefore, the purpose of the present study was to understand the effects of HSD on the development and function of NK cells, in addition to investigating the underlying molecular mechanism. Our results show that the number of NK cells in the spleen and lungs of HSD-fed mice was significantly reduced, which may be due to the inhibition of NK cell proliferation. Further, the development of NK cells in mice was evaluated, and it was found that HSD reduced the effective NK cell subset (CD27+CD11b-). Moreover, it was also found that the ability of NK cells to secrete CD107a and IFN-γ in HSD-fed mice was decreased following stimulation with RMA-S and YAC-1 tumor cells. Finally, the underlying molecular mechanism was evaluated, and it was found that HSD increased the production of reactive oxygen species (ROS) by NK cells, while the expression of CD122 was decreased, suggesting that HSD downregulates CD122 expression in NK cells via ROS signaling, thereby reducing the responsiveness to IL-15 and ultimately inhibiting NK cell function. The present research discovered a novel mechanism by which HSD inhibits the function of NK cells, providing an alternative avenue for the treatment of immune diseases caused by HSD. |
| format | Article |
| id | doaj-art-c7b5ef106b6c43e59025d699a916bf59 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-c7b5ef106b6c43e59025d699a916bf592025-02-03T06:43:36ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/66871436687143A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in MiceXiaokang Zeng0Yan Li1Weibiao Lv2Xinhuai Dong3Chong Zeng4Liming Zeng5Zibo Wei6Xu Lin7Yanning Ma8Qiang Xiao9Central Laboratory, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaClinical Medicine Research Center, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaClinical Laboratory, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaCentral Laboratory, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaCentral Laboratory, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaCentral Laboratory, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaCentral Laboratory, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaCentral Laboratory, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaRadiotherapy Department, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, Guangdong, ChinaPulmonary and Critical Care Medicine, Shunde Hospital, Southern Medical University (The First People’s Hospital of Shunde Foshan), Foshan, 528300 Guangdong, ChinaA high-salt diet (HSD) is common worldwide and can lead to cardiovascular disease, chronic inflammation, and autoimmune diseases. Moreover, increasing evidence shows that HSD is closely related to a variety of immune diseases. Natural killer (NK) cells are important innate immune cells that directly kill their targets via degranulation and secretion of interferon gamma (IFN-γ). NK cells play a vital role in resisting viruses and preventing the malignant transformation of cells; however, whether HSD affects the development and function of NK cells has not yet been elucidated. Therefore, the purpose of the present study was to understand the effects of HSD on the development and function of NK cells, in addition to investigating the underlying molecular mechanism. Our results show that the number of NK cells in the spleen and lungs of HSD-fed mice was significantly reduced, which may be due to the inhibition of NK cell proliferation. Further, the development of NK cells in mice was evaluated, and it was found that HSD reduced the effective NK cell subset (CD27+CD11b-). Moreover, it was also found that the ability of NK cells to secrete CD107a and IFN-γ in HSD-fed mice was decreased following stimulation with RMA-S and YAC-1 tumor cells. Finally, the underlying molecular mechanism was evaluated, and it was found that HSD increased the production of reactive oxygen species (ROS) by NK cells, while the expression of CD122 was decreased, suggesting that HSD downregulates CD122 expression in NK cells via ROS signaling, thereby reducing the responsiveness to IL-15 and ultimately inhibiting NK cell function. The present research discovered a novel mechanism by which HSD inhibits the function of NK cells, providing an alternative avenue for the treatment of immune diseases caused by HSD.http://dx.doi.org/10.1155/2020/6687143 |
| spellingShingle | Xiaokang Zeng Yan Li Weibiao Lv Xinhuai Dong Chong Zeng Liming Zeng Zibo Wei Xu Lin Yanning Ma Qiang Xiao A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice Journal of Immunology Research |
| title | A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice |
| title_full | A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice |
| title_fullStr | A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice |
| title_full_unstemmed | A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice |
| title_short | A High-Salt Diet Disturbs the Development and Function of Natural Killer Cells in Mice |
| title_sort | high salt diet disturbs the development and function of natural killer cells in mice |
| url | http://dx.doi.org/10.1155/2020/6687143 |
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