Morphine and codeine in racing horse feed: is there reason for concern?
Opiates such as morphine and codeine are substances often misused to improve the performance of racing horses during competitions and are therefore on the International Federation for Equestrian Sports’ list of prohibited substances. However, a positive antidoping test may be due to the consumption...
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| Format: | Article |
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Sciendo
2025-03-01
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| Series: | Arhiv za Higijenu Rada i Toksikologiju |
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| Online Access: | https://doi.org/10.2478/aiht-2025-76-3888 |
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| author | Karačonji Irena Brčić Jelača Tea Jurič Andreja Vrdoljak Ana Lucić |
| author_facet | Karačonji Irena Brčić Jelača Tea Jurič Andreja Vrdoljak Ana Lucić |
| author_sort | Karačonji Irena Brčić |
| collection | DOAJ |
| description | Opiates such as morphine and codeine are substances often misused to improve the performance of racing horses during competitions and are therefore on the International Federation for Equestrian Sports’ list of prohibited substances. However, a positive antidoping test may be due to the consumption of feed (mainly lucerne or oats) contaminated by opium poppy containing the alkaloids morphine and codeine. In order to determine whether a positive antidoping test is the result of an intentional abuse of opiates or consumption of feed contaminated by poppies, we optimised conditions for the extraction of morphine and codeine from dehydrated lucerne and developed and validated a gas chromatography-mass spectrometry (GC-MS) method for the simultaneous determination of both analytes. The most efficient extraction of morphine and codeine from dehydrated lucerne was achieved using a citrate buffer pH4 followed by solid phase extraction. The method showed satisfactory linearity (R2>0.9980) in the tested concentration range (85–1600 ng/g), as well as good precision (RSD<4 %), accuracy (>95 %), and sensitivity (limit of detection 22 and 25 ng/g for morphine and codeine, respectively). The proposed method was used for analysing a sample of dehydrated lucerne having measurable content of morphine (1510 ng/g) and codeine (327 ng/g) that can cause positive results of opiate blood or urine testing up to 4 hours after feeding the horse with less than 500 g of dehydrated lucerne. The use of this analytical method should enable the exclusion of horse feed as the cause of positive antidoping tests. |
| format | Article |
| id | doaj-art-c7adcf7e916048e7afba4066879d052d |
| institution | OA Journals |
| issn | 1848-6312 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Arhiv za Higijenu Rada i Toksikologiju |
| spelling | doaj-art-c7adcf7e916048e7afba4066879d052d2025-08-20T02:11:54ZengSciendoArhiv za Higijenu Rada i Toksikologiju1848-63122025-03-01761606610.2478/aiht-2025-76-3888Morphine and codeine in racing horse feed: is there reason for concern?Karačonji Irena Brčić0Jelača Tea1Jurič Andreja2Vrdoljak Ana Lucić3Institute for Medical Research and Occupational Health, Zagreb, CroatiaUniversity of Rijeka , Department of Biotechnology , Rijeka , CroatiaInstitute for Medical Research and Occupational Health, Zagreb, CroatiaInstitute for Medical Research and Occupational Health, Zagreb, CroatiaOpiates such as morphine and codeine are substances often misused to improve the performance of racing horses during competitions and are therefore on the International Federation for Equestrian Sports’ list of prohibited substances. However, a positive antidoping test may be due to the consumption of feed (mainly lucerne or oats) contaminated by opium poppy containing the alkaloids morphine and codeine. In order to determine whether a positive antidoping test is the result of an intentional abuse of opiates or consumption of feed contaminated by poppies, we optimised conditions for the extraction of morphine and codeine from dehydrated lucerne and developed and validated a gas chromatography-mass spectrometry (GC-MS) method for the simultaneous determination of both analytes. The most efficient extraction of morphine and codeine from dehydrated lucerne was achieved using a citrate buffer pH4 followed by solid phase extraction. The method showed satisfactory linearity (R2>0.9980) in the tested concentration range (85–1600 ng/g), as well as good precision (RSD<4 %), accuracy (>95 %), and sensitivity (limit of detection 22 and 25 ng/g for morphine and codeine, respectively). The proposed method was used for analysing a sample of dehydrated lucerne having measurable content of morphine (1510 ng/g) and codeine (327 ng/g) that can cause positive results of opiate blood or urine testing up to 4 hours after feeding the horse with less than 500 g of dehydrated lucerne. The use of this analytical method should enable the exclusion of horse feed as the cause of positive antidoping tests.https://doi.org/10.2478/aiht-2025-76-3888antidopinggc-mslucernemethod developmentopiatesantidopinggc-mslucernaopijatirazvoj metode |
| spellingShingle | Karačonji Irena Brčić Jelača Tea Jurič Andreja Vrdoljak Ana Lucić Morphine and codeine in racing horse feed: is there reason for concern? Arhiv za Higijenu Rada i Toksikologiju antidoping gc-ms lucerne method development opiates antidoping gc-ms lucerna opijati razvoj metode |
| title | Morphine and codeine in racing horse feed: is there reason for concern? |
| title_full | Morphine and codeine in racing horse feed: is there reason for concern? |
| title_fullStr | Morphine and codeine in racing horse feed: is there reason for concern? |
| title_full_unstemmed | Morphine and codeine in racing horse feed: is there reason for concern? |
| title_short | Morphine and codeine in racing horse feed: is there reason for concern? |
| title_sort | morphine and codeine in racing horse feed is there reason for concern |
| topic | antidoping gc-ms lucerne method development opiates antidoping gc-ms lucerna opijati razvoj metode |
| url | https://doi.org/10.2478/aiht-2025-76-3888 |
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