Characterization and exopolysaccharide production of Bacillus velezensis DTA1 from Vietnamese atoll soil

Exopolysaccharides (EPS) and salt-tolerant, EPS-producing bacteria have attracted increasing attention due to their diverse applications across various fields. This study investigated the characteristics and EPS production potential of Bacillus velezensis DTA1, a salt-tolerant, EPS-producing strain...

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Main Authors: Thi Hue Le, Hoang Tuan Dinh, Mai Huong Le, Cong Tinh Nguyen, Quang Cuong Hoang
Format: Article
Language:English
Published: University of Brawijaya 2025-07-01
Series:Journal of Degraded and Mining Lands Management
Subjects:
Online Access:https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/17192
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author Thi Hue Le
Hoang Tuan Dinh
Mai Huong Le
Cong Tinh Nguyen
Quang Cuong Hoang
author_facet Thi Hue Le
Hoang Tuan Dinh
Mai Huong Le
Cong Tinh Nguyen
Quang Cuong Hoang
author_sort Thi Hue Le
collection DOAJ
description Exopolysaccharides (EPS) and salt-tolerant, EPS-producing bacteria have attracted increasing attention due to their diverse applications across various fields. This study investigated the characteristics and EPS production potential of Bacillus velezensis DTA1, a salt-tolerant, EPS-producing strain isolated from coral island soil in Vietnam. Strain DTA1 demonstrated notable versatility in utilizing various carbon sources and exhibited a wide tolerance range to temperature, pH, and NaCl concentrations. Response Surface Methodology (RSM) was employed to optimize the culture conditions, resulting in an EPS yield of 32.80 g/L, achieved under optimized conditions of 7.26% sucrose, 3.27% NaCl, and pH 8.46. Through the evaluation of water-holding capacity, this study demonstrated the notable properties of the EPS and culture broth of strain DTA1 in enhancing water retention and soil component aggregation. These findings highlight the potential of strain DTA1 for arid land reclamation applications, particularly in harsh environments such as saline, alkaline, and arid coral sand areas on offshore islands.
format Article
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institution Kabale University
issn 2339-076X
2502-2458
language English
publishDate 2025-07-01
publisher University of Brawijaya
record_format Article
series Journal of Degraded and Mining Lands Management
spelling doaj-art-c7ad2fcb9bcf46d396cf028e9248a0952025-08-20T03:29:57ZengUniversity of BrawijayaJournal of Degraded and Mining Lands Management2339-076X2502-24582025-07-011248305831410.15243/jdmlm.2025.124.830517194Characterization and exopolysaccharide production of Bacillus velezensis DTA1 from Vietnamese atoll soilThi Hue Le0https://orcid.org/0009-0002-2722-5798Hoang Tuan Dinh1Mai Huong Le2https://orcid.org/0000-0002-5070-7421Cong Tinh Nguyen3Quang Cuong Hoang4Joint Vietnam-Russia Tropical Science and Technology Research Center, 63 Nguyen Van Huyen, Nghia Do, Cau Giay, Hanoi, Vietnam VNU University of Science, 334 Nguyen Trai, Thanh Xuan, Hanoi, VietnamInstitute of Chemistry, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Cau Giay, Hanoi, Vietnam Joint Vietnam-Russia Tropical Science and Technology Research Center, 63 Nguyen Van Huyen, Nghia Do, Cau Giay, Hanoi, Vietnam Joint Vietnam-Russia Tropical Science and Technology Research Center, 63 Nguyen Van Huyen, Nghia Do, Cau Giay, Hanoi, Vietnam Exopolysaccharides (EPS) and salt-tolerant, EPS-producing bacteria have attracted increasing attention due to their diverse applications across various fields. This study investigated the characteristics and EPS production potential of Bacillus velezensis DTA1, a salt-tolerant, EPS-producing strain isolated from coral island soil in Vietnam. Strain DTA1 demonstrated notable versatility in utilizing various carbon sources and exhibited a wide tolerance range to temperature, pH, and NaCl concentrations. Response Surface Methodology (RSM) was employed to optimize the culture conditions, resulting in an EPS yield of 32.80 g/L, achieved under optimized conditions of 7.26% sucrose, 3.27% NaCl, and pH 8.46. Through the evaluation of water-holding capacity, this study demonstrated the notable properties of the EPS and culture broth of strain DTA1 in enhancing water retention and soil component aggregation. These findings highlight the potential of strain DTA1 for arid land reclamation applications, particularly in harsh environments such as saline, alkaline, and arid coral sand areas on offshore islands.https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/17192bacillus velezensisexopolysaccharidesresponse surface methodologysalt-tolerant
spellingShingle Thi Hue Le
Hoang Tuan Dinh
Mai Huong Le
Cong Tinh Nguyen
Quang Cuong Hoang
Characterization and exopolysaccharide production of Bacillus velezensis DTA1 from Vietnamese atoll soil
Journal of Degraded and Mining Lands Management
bacillus velezensis
exopolysaccharides
response surface methodology
salt-tolerant
title Characterization and exopolysaccharide production of Bacillus velezensis DTA1 from Vietnamese atoll soil
title_full Characterization and exopolysaccharide production of Bacillus velezensis DTA1 from Vietnamese atoll soil
title_fullStr Characterization and exopolysaccharide production of Bacillus velezensis DTA1 from Vietnamese atoll soil
title_full_unstemmed Characterization and exopolysaccharide production of Bacillus velezensis DTA1 from Vietnamese atoll soil
title_short Characterization and exopolysaccharide production of Bacillus velezensis DTA1 from Vietnamese atoll soil
title_sort characterization and exopolysaccharide production of bacillus velezensis dta1 from vietnamese atoll soil
topic bacillus velezensis
exopolysaccharides
response surface methodology
salt-tolerant
url https://jdmlm.ub.ac.id/index.php/jdmlm/article/view/17192
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AT maihuongle characterizationandexopolysaccharideproductionofbacillusvelezensisdta1fromvietnameseatollsoil
AT congtinhnguyen characterizationandexopolysaccharideproductionofbacillusvelezensisdta1fromvietnameseatollsoil
AT quangcuonghoang characterizationandexopolysaccharideproductionofbacillusvelezensisdta1fromvietnameseatollsoil