Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells
In situ detection of MSCs remains difficult and warrants additional methods to aid with their characterization in vivo. Two-photon confocal laser scanning microscopy (TPM) and second harmonic generation (SHG) could fill this gap. Both techniques enable the detection of cells and extracellular struct...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/5457132 |
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| _version_ | 1849685858612936704 |
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| author | Raf Donders Kathleen Sanen Rik Paesen Eli Slenders Wilfried Gyselaers Piet Stinissen Marcel Ameloot Niels Hellings |
| author_facet | Raf Donders Kathleen Sanen Rik Paesen Eli Slenders Wilfried Gyselaers Piet Stinissen Marcel Ameloot Niels Hellings |
| author_sort | Raf Donders |
| collection | DOAJ |
| description | In situ detection of MSCs remains difficult and warrants additional methods to aid with their characterization in vivo. Two-photon confocal laser scanning microscopy (TPM) and second harmonic generation (SHG) could fill this gap. Both techniques enable the detection of cells and extracellular structures, based on intrinsic properties of the specific tissue and intracellular molecules under optical irradiation. TPM imaging and SHG imaging have been used for label-free monitoring of stem cells differentiation, assessment of their behavior in biocompatible scaffolds, and even cell tracking in vivo. In this study, we show that TPM and SHG can accurately depict the umbilical cord architecture and visualize individual cells both in situ and during culture initiation, without the use of exogenously applied labels. In combination with nuclear DNA staining, we observed a variance in fluorescent intensity in the vessel walls. In addition, antibody staining showed differences in Oct4, αSMA, vimentin, and ALDH1A1 expression in situ, indicating functional differences among the umbilical cord cell populations. In future research, marker-free imaging can be of great added value to the current antigen-based staining methods for describing tissue structures and for the identification of progenitor cells in their tissue of origin. |
| format | Article |
| id | doaj-art-c7a32d0eca8141f6befd861df3a24b27 |
| institution | DOAJ |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-c7a32d0eca8141f6befd861df3a24b272025-08-20T03:22:57ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/54571325457132Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived CellsRaf Donders0Kathleen Sanen1Rik Paesen2Eli Slenders3Wilfried Gyselaers4Piet Stinissen5Marcel Ameloot6Niels Hellings7Biomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, Agoralaan Building C, 3590 Diepenbeek, BelgiumBiomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, Agoralaan Building C, 3590 Diepenbeek, BelgiumBiomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, Agoralaan Building C, 3590 Diepenbeek, BelgiumBiomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, Agoralaan Building C, 3590 Diepenbeek, BelgiumBiomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, Agoralaan Building C, 3590 Diepenbeek, BelgiumBiomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, Agoralaan Building C, 3590 Diepenbeek, BelgiumBiomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, Agoralaan Building C, 3590 Diepenbeek, BelgiumBiomedical Research Institute, Hasselt University and School of Life Sciences, Transnational University Limburg, Agoralaan Building C, 3590 Diepenbeek, BelgiumIn situ detection of MSCs remains difficult and warrants additional methods to aid with their characterization in vivo. Two-photon confocal laser scanning microscopy (TPM) and second harmonic generation (SHG) could fill this gap. Both techniques enable the detection of cells and extracellular structures, based on intrinsic properties of the specific tissue and intracellular molecules under optical irradiation. TPM imaging and SHG imaging have been used for label-free monitoring of stem cells differentiation, assessment of their behavior in biocompatible scaffolds, and even cell tracking in vivo. In this study, we show that TPM and SHG can accurately depict the umbilical cord architecture and visualize individual cells both in situ and during culture initiation, without the use of exogenously applied labels. In combination with nuclear DNA staining, we observed a variance in fluorescent intensity in the vessel walls. In addition, antibody staining showed differences in Oct4, αSMA, vimentin, and ALDH1A1 expression in situ, indicating functional differences among the umbilical cord cell populations. In future research, marker-free imaging can be of great added value to the current antigen-based staining methods for describing tissue structures and for the identification of progenitor cells in their tissue of origin.http://dx.doi.org/10.1155/2016/5457132 |
| spellingShingle | Raf Donders Kathleen Sanen Rik Paesen Eli Slenders Wilfried Gyselaers Piet Stinissen Marcel Ameloot Niels Hellings Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells Stem Cells International |
| title | Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells |
| title_full | Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells |
| title_fullStr | Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells |
| title_full_unstemmed | Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells |
| title_short | Label-Free Imaging of Umbilical Cord Tissue Morphology and Explant-Derived Cells |
| title_sort | label free imaging of umbilical cord tissue morphology and explant derived cells |
| url | http://dx.doi.org/10.1155/2016/5457132 |
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