EVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTS
In this study 122 strains of strictly anaerobic gram-negative rods Bacteroides, Prevotella and Fusobacterium, isolated from cancer patients between 2004 and 2014 were analyzed. The most common strains were B. fragilis (55 %) followed by Prevotella (34.4 %) and Fusobacterium spp (10.6 %). The species...
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Russian Academy of Sciences, Tomsk National Research Medical Center
2016-02-01
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| Series: | Сибирский онкологический журнал |
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| Online Access: | https://www.siboncoj.ru/jour/article/view/148 |
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| author | I. I. Shilnikova N. V. Dmitrieva |
| author_facet | I. I. Shilnikova N. V. Dmitrieva |
| author_sort | I. I. Shilnikova |
| collection | DOAJ |
| description | In this study 122 strains of strictly anaerobic gram-negative rods Bacteroides, Prevotella and Fusobacterium, isolated from cancer patients between 2004 and 2014 were analyzed. The most common strains were B. fragilis (55 %) followed by Prevotella (34.4 %) and Fusobacterium spp (10.6 %). The species identification was carried out by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Among the B. fragilis group strains, B. fragilis, B. thetaiotaomicron, B. ovatus and B. vulgatus were isolated most frequently. Among Prevotella species, the most frequently isolated species were P. buccae, P. buccalis, P. oris, P. denticola and P. nigrescens, and 77 % of the Fusobacterium spp. were F. nucleatum. Susceptibilities of the isolates to antimicrobial agents were determined by the E-test methodology. The percentage of the susceptibility of B. fragilis group isolates were: metronidazole, 97 %; imipenem, 95.5 %; amoxicillin/clavulanate, 95.5 % and clindamycin, 77.6 %. Three B. fragilis isolates proved to be multidrug-resistant (simultaneously to imipenem, amoxicillin/ clavulanate and metronidazole or clindamycin). All Prevotella isolates were susceptible to imipenem and amoxicillin/clavulanate, whereas 78.6 % of the pigmented Prevotella species and 46.4 % of the non-pigmented species were resistant to penicillin. The number of strains susceptibility to metronidazole and clindamycin were 93 % and 88 %, respectively. All Fusobacterium strains were sensitive to all tested antibiotics, including penicillin. |
| format | Article |
| id | doaj-art-c7a2c089f9df4ae5bf6a5b746fef361a |
| institution | Kabale University |
| issn | 1814-4861 2312-3168 |
| language | Russian |
| publishDate | 2016-02-01 |
| publisher | Russian Academy of Sciences, Tomsk National Research Medical Center |
| record_format | Article |
| series | Сибирский онкологический журнал |
| spelling | doaj-art-c7a2c089f9df4ae5bf6a5b746fef361a2025-08-20T03:56:24ZrusRussian Academy of Sciences, Tomsk National Research Medical CenterСибирский онкологический журнал1814-48612312-31682016-02-01153743148EVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTSI. I. Shilnikova0N. V. Dmitrieva1N.N. Blokhin Russian Cancer Research Center of the Russian Academy of Medical Sciences, MoscowN.N. Blokhin Russian Cancer Research Center of the Russian Academy of Medical Sciences, MoscowIn this study 122 strains of strictly anaerobic gram-negative rods Bacteroides, Prevotella and Fusobacterium, isolated from cancer patients between 2004 and 2014 were analyzed. The most common strains were B. fragilis (55 %) followed by Prevotella (34.4 %) and Fusobacterium spp (10.6 %). The species identification was carried out by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Among the B. fragilis group strains, B. fragilis, B. thetaiotaomicron, B. ovatus and B. vulgatus were isolated most frequently. Among Prevotella species, the most frequently isolated species were P. buccae, P. buccalis, P. oris, P. denticola and P. nigrescens, and 77 % of the Fusobacterium spp. were F. nucleatum. Susceptibilities of the isolates to antimicrobial agents were determined by the E-test methodology. The percentage of the susceptibility of B. fragilis group isolates were: metronidazole, 97 %; imipenem, 95.5 %; amoxicillin/clavulanate, 95.5 % and clindamycin, 77.6 %. Three B. fragilis isolates proved to be multidrug-resistant (simultaneously to imipenem, amoxicillin/ clavulanate and metronidazole or clindamycin). All Prevotella isolates were susceptible to imipenem and amoxicillin/clavulanate, whereas 78.6 % of the pigmented Prevotella species and 46.4 % of the non-pigmented species were resistant to penicillin. The number of strains susceptibility to metronidazole and clindamycin were 93 % and 88 %, respectively. All Fusobacterium strains were sensitive to all tested antibiotics, including penicillin.https://www.siboncoj.ru/jour/article/view/148antimicrobial susceptibility, resistance, bacteroides, prevotella, fusobacterium, cancer patients |
| spellingShingle | I. I. Shilnikova N. V. Dmitrieva EVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTS Сибирский онкологический журнал antimicrobial susceptibility, resistance, bacteroides, prevotella, fusobacterium, cancer patients |
| title | EVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTS |
| title_full | EVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTS |
| title_fullStr | EVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTS |
| title_full_unstemmed | EVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTS |
| title_short | EVALUATION OF ANTIBIOTIC SUSCEPTIBILITY OF ANAEROBIC PATHOGENS BACTEROIDES, PREVOTELLA AND FUSOBACTERIUM ISOLATED FROM CANCER PATIENTS |
| title_sort | evaluation of antibiotic susceptibility of anaerobic pathogens bacteroides prevotella and fusobacterium isolated from cancer patients |
| topic | antimicrobial susceptibility, resistance, bacteroides, prevotella, fusobacterium, cancer patients |
| url | https://www.siboncoj.ru/jour/article/view/148 |
| work_keys_str_mv | AT iishilnikova evaluationofantibioticsusceptibilityofanaerobicpathogensbacteroidesprevotellaandfusobacteriumisolatedfromcancerpatients AT nvdmitrieva evaluationofantibioticsusceptibilityofanaerobicpathogensbacteroidesprevotellaandfusobacteriumisolatedfromcancerpatients |