Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae
To investigate the expression and distribution of interferon-γ (IFN-γ) in lung and trachea of macaques infected with Streptococcus pneumoniae, the histopathological changes of macaques lung and trachea were studied by using hematoxylin-eosin (HE) stain, where the expression of IFN-γ was researched b...
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Zhejiang University Press
2012-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.01.007 |
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| author | LUO Qi-hui ZHANG Yao CHEN Lian CHEN Zheng-li ZENG Wen CHENG An-chun |
| author_facet | LUO Qi-hui ZHANG Yao CHEN Lian CHEN Zheng-li ZENG Wen CHENG An-chun |
| author_sort | LUO Qi-hui |
| collection | DOAJ |
| description | To investigate the expression and distribution of interferon-γ (IFN-γ) in lung and trachea of macaques infected with Streptococcus pneumoniae, the histopathological changes of macaques lung and trachea were studied by using hematoxylin-eosin (HE) stain, where the expression of IFN-γ was researched by immunohistochemistry and in situ hybridization. The results showed that a large area of lung was hyperemic and hemorrhagic, and there was lymphocytic infiltration in lung and some serous exudate in alveolar septum. The area of IFN-γ protein expression in infected macaques was higher than healthy group (P<0.05). The optical density of IFN-γ protein in pulmonary intravascular space was significantly higher than that of healthy group (P<0.01), and the optical density in other tissues was higher than that of healthy group (P<0.05). The optical densities of IFN-γ mRNA in lung, tracheal epithelium and muscular layer were significantly higher than those of healthy group (P<0.01), while in alveolar septum, lumen of blood vessel and lamina propria of tracheal were higher than those of healthy group (P<0.05). As a result, the lung presented significant red hepatization in infected macaques, and the expression of IFN-γ protein and mRNA in lung and trachea increased significantly, indicating that IFN-γ might play an important role in immunoloregulation of macaques lung and trachea infected by S. pneumoniae. |
| format | Article |
| id | doaj-art-c7a244af79304da381f30f63f618e227 |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-c7a244af79304da381f30f63f618e2272025-08-20T03:16:03ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552012-01-0138485410.3785/j.issn.1008-9209.2012.01.00710089209Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniaeLUO Qi-huiZHANG YaoCHEN LianCHEN Zheng-liZENG WenCHENG An-chunTo investigate the expression and distribution of interferon-γ (IFN-γ) in lung and trachea of macaques infected with Streptococcus pneumoniae, the histopathological changes of macaques lung and trachea were studied by using hematoxylin-eosin (HE) stain, where the expression of IFN-γ was researched by immunohistochemistry and in situ hybridization. The results showed that a large area of lung was hyperemic and hemorrhagic, and there was lymphocytic infiltration in lung and some serous exudate in alveolar septum. The area of IFN-γ protein expression in infected macaques was higher than healthy group (P<0.05). The optical density of IFN-γ protein in pulmonary intravascular space was significantly higher than that of healthy group (P<0.01), and the optical density in other tissues was higher than that of healthy group (P<0.05). The optical densities of IFN-γ mRNA in lung, tracheal epithelium and muscular layer were significantly higher than those of healthy group (P<0.01), while in alveolar septum, lumen of blood vessel and lamina propria of tracheal were higher than those of healthy group (P<0.05). As a result, the lung presented significant red hepatization in infected macaques, and the expression of IFN-γ protein and mRNA in lung and trachea increased significantly, indicating that IFN-γ might play an important role in immunoloregulation of macaques lung and trachea infected by S. pneumoniae.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.01.007<italic>Streptococcus pneumoniae</italic>interferon-<italic>γ</italic>immunohistochemistrytissue <italic>in situ</italic> hybridizationlungtracheamacaque |
| spellingShingle | LUO Qi-hui ZHANG Yao CHEN Lian CHEN Zheng-li ZENG Wen CHENG An-chun Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae 浙江大学学报. 农业与生命科学版 <italic>Streptococcus pneumoniae</italic> interferon-<italic>γ</italic> immunohistochemistry tissue <italic>in situ</italic> hybridization lung trachea macaque |
| title | Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae |
| title_full | Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae |
| title_fullStr | Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae |
| title_full_unstemmed | Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae |
| title_short | Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae |
| title_sort | expression of ifn γ protein and mrna in lung and trachea of macaque infected with streptococcus pneumoniae |
| topic | <italic>Streptococcus pneumoniae</italic> interferon-<italic>γ</italic> immunohistochemistry tissue <italic>in situ</italic> hybridization lung trachea macaque |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.01.007 |
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