Expression of IFN-γ protein and mRNA in lung and trachea of macaque infected with Streptococcus pneumoniae
To investigate the expression and distribution of interferon-γ (IFN-γ) in lung and trachea of macaques infected with Streptococcus pneumoniae, the histopathological changes of macaques lung and trachea were studied by using hematoxylin-eosin (HE) stain, where the expression of IFN-γ was researched b...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2012-01-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.01.007 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | To investigate the expression and distribution of interferon-γ (IFN-γ) in lung and trachea of macaques infected with Streptococcus pneumoniae, the histopathological changes of macaques lung and trachea were studied by using hematoxylin-eosin (HE) stain, where the expression of IFN-γ was researched by immunohistochemistry and in situ hybridization. The results showed that a large area of lung was hyperemic and hemorrhagic, and there was lymphocytic infiltration in lung and some serous exudate in alveolar septum. The area of IFN-γ protein expression in infected macaques was higher than healthy group (P<0.05). The optical density of IFN-γ protein in pulmonary intravascular space was significantly higher than that of healthy group (P<0.01), and the optical density in other tissues was higher than that of healthy group (P<0.05). The optical densities of IFN-γ mRNA in lung, tracheal epithelium and muscular layer were significantly higher than those of healthy group (P<0.01), while in alveolar septum, lumen of blood vessel and lamina propria of tracheal were higher than those of healthy group (P<0.05). As a result, the lung presented significant red hepatization in infected macaques, and the expression of IFN-γ protein and mRNA in lung and trachea increased significantly, indicating that IFN-γ might play an important role in immunoloregulation of macaques lung and trachea infected by S. pneumoniae. |
|---|---|
| ISSN: | 1008-9209 2097-5155 |