Sequential extraction of antioxidants from Citrus aurantium L. flower and kinetic evaluation of their effect on oil microparticle oxidative stability
This study applied supercritical CO2 and ultrasound-assisted ethanol extraction (UAE) to obtain apolar and polar antioxidant fractions from Citrus aurantium flowers. HPLC-HRMS revealed distinct phytochemical profiles, with the polar extract showing higher phenolic content and antioxidant activity. L...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-04-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525002950 |
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| author | Abdessamie Kellil Rajat Suhag Maria Concetta Tenuta Sara Bolchini Vakare Merkyte Matteo Scampicchio Ksenia Morozova Giovanna Ferrentino |
| author_facet | Abdessamie Kellil Rajat Suhag Maria Concetta Tenuta Sara Bolchini Vakare Merkyte Matteo Scampicchio Ksenia Morozova Giovanna Ferrentino |
| author_sort | Abdessamie Kellil |
| collection | DOAJ |
| description | This study applied supercritical CO2 and ultrasound-assisted ethanol extraction (UAE) to obtain apolar and polar antioxidant fractions from Citrus aurantium flowers. HPLC-HRMS revealed distinct phytochemical profiles, with the polar extract showing higher phenolic content and antioxidant activity. Linseed oil was co-encapsulated with these extracts at varying concentrations (1, 2, and 5 mg/g of oil) using the Particles from Gas Saturated Solution (PGSS) technique, and the oxidative stability was assessed using isothermal calorimetry. Key kinetic parameters, such as induction time (τ), oxidation rates (Rinh, Runi), and antioxidant efficiency (A.E.), were measured. The polar extract demonstrated superior A.E., further confirmed by the DPPH stopped-flow assay. Co-encapsulation of both extracts produced an additive effect, surpassing the synthetic antioxidant BHT (200 μg/g). This study highlights Citrus aurantium flower extracts as natural antioxidants, enhancing the oxidative stability of encapsulated oils, while offering a sustainable method for bioactive compound recovery for food applications. |
| format | Article |
| id | doaj-art-c7a121e8c0dd4098ba047f014bfee3d1 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-c7a121e8c0dd4098ba047f014bfee3d12025-08-20T03:49:45ZengElsevierFood Chemistry: X2590-15752025-04-012710244810.1016/j.fochx.2025.102448Sequential extraction of antioxidants from Citrus aurantium L. flower and kinetic evaluation of their effect on oil microparticle oxidative stabilityAbdessamie Kellil0Rajat Suhag1Maria Concetta Tenuta2Sara Bolchini3Vakare Merkyte4Matteo Scampicchio5Ksenia Morozova6Giovanna Ferrentino7Faculty of Agricultural, Environmental and Food Sciences, Free University of Bolzano, Piazza Università, 1, Bolzano 39100, ItalyFaculty of Agricultural, Environmental and Food Sciences, Free University of Bolzano, Piazza Università, 1, Bolzano 39100, ItalyFaculty of Agricultural, Environmental and Food Sciences, Free University of Bolzano, Piazza Università, 1, Bolzano 39100, ItalyFaculty of Agricultural, Environmental and Food Sciences, Free University of Bolzano, Piazza Università, 1, Bolzano 39100, ItalyNestlé NPTC Waters, Via Dogana Vecchia snc, 24040 Madone, ItalyFaculty of Agricultural, Environmental and Food Sciences, Free University of Bolzano, Piazza Università, 1, Bolzano 39100, ItalyFaculty of Agricultural, Environmental and Food Sciences, Free University of Bolzano, Piazza Università, 1, Bolzano 39100, ItalyFaculty of Agricultural, Environmental and Food Sciences, Free University of Bolzano, Piazza Università, 1, Bolzano 39100, Italy; Corresponding author.This study applied supercritical CO2 and ultrasound-assisted ethanol extraction (UAE) to obtain apolar and polar antioxidant fractions from Citrus aurantium flowers. HPLC-HRMS revealed distinct phytochemical profiles, with the polar extract showing higher phenolic content and antioxidant activity. Linseed oil was co-encapsulated with these extracts at varying concentrations (1, 2, and 5 mg/g of oil) using the Particles from Gas Saturated Solution (PGSS) technique, and the oxidative stability was assessed using isothermal calorimetry. Key kinetic parameters, such as induction time (τ), oxidation rates (Rinh, Runi), and antioxidant efficiency (A.E.), were measured. The polar extract demonstrated superior A.E., further confirmed by the DPPH stopped-flow assay. Co-encapsulation of both extracts produced an additive effect, surpassing the synthetic antioxidant BHT (200 μg/g). This study highlights Citrus aurantium flower extracts as natural antioxidants, enhancing the oxidative stability of encapsulated oils, while offering a sustainable method for bioactive compound recovery for food applications.http://www.sciencedirect.com/science/article/pii/S2590157525002950Supercritical fluid technologiesUltrasound assisted extractionAntioxidant activityEncapsulationGreen technologies |
| spellingShingle | Abdessamie Kellil Rajat Suhag Maria Concetta Tenuta Sara Bolchini Vakare Merkyte Matteo Scampicchio Ksenia Morozova Giovanna Ferrentino Sequential extraction of antioxidants from Citrus aurantium L. flower and kinetic evaluation of their effect on oil microparticle oxidative stability Food Chemistry: X Supercritical fluid technologies Ultrasound assisted extraction Antioxidant activity Encapsulation Green technologies |
| title | Sequential extraction of antioxidants from Citrus aurantium L. flower and kinetic evaluation of their effect on oil microparticle oxidative stability |
| title_full | Sequential extraction of antioxidants from Citrus aurantium L. flower and kinetic evaluation of their effect on oil microparticle oxidative stability |
| title_fullStr | Sequential extraction of antioxidants from Citrus aurantium L. flower and kinetic evaluation of their effect on oil microparticle oxidative stability |
| title_full_unstemmed | Sequential extraction of antioxidants from Citrus aurantium L. flower and kinetic evaluation of their effect on oil microparticle oxidative stability |
| title_short | Sequential extraction of antioxidants from Citrus aurantium L. flower and kinetic evaluation of their effect on oil microparticle oxidative stability |
| title_sort | sequential extraction of antioxidants from citrus aurantium l flower and kinetic evaluation of their effect on oil microparticle oxidative stability |
| topic | Supercritical fluid technologies Ultrasound assisted extraction Antioxidant activity Encapsulation Green technologies |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525002950 |
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