Biological properties and vector competence of Diaphorina citri for Candidatus Liberibacter asiaticus modulated by an insect-specific virus

Summary: Insect-specific viruses (ISVs) infect only insects and have been studied primarily in mosquitoes. Here, we extend the concept of ISVs to include those found in plant-feeding insect vectors, such as Diaphorina citri, the vector of Candidatus Liberibacter asiaticus (CLas), the causal agent of...

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Main Authors: Diogo M. Galdeano, Tanvi Rawat, William Ingram, Curtis R. Carlson, Gustavo Rodrigues Alves, Yen-Wen Kuo
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:iScience
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Online Access:http://www.sciencedirect.com/science/article/pii/S258900422501243X
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author Diogo M. Galdeano
Tanvi Rawat
William Ingram
Curtis R. Carlson
Gustavo Rodrigues Alves
Yen-Wen Kuo
author_facet Diogo M. Galdeano
Tanvi Rawat
William Ingram
Curtis R. Carlson
Gustavo Rodrigues Alves
Yen-Wen Kuo
author_sort Diogo M. Galdeano
collection DOAJ
description Summary: Insect-specific viruses (ISVs) infect only insects and have been studied primarily in mosquitoes. Here, we extend the concept of ISVs to include those found in plant-feeding insect vectors, such as Diaphorina citri, the vector of Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus Huanglongbing. Using Diaphorina citri flavi-like virus (DcFLV) as a model, we examined its effects on host biology and CLas transmission. DcFLV infection delayed development and preoviposition and reduced feeding activity, whereas overall survival, oviposition, and sex ratio were unaffected. DcFLV-infected D. citri transmitted CLas with ∼20% greater efficiency than uninfected controls, despite no significant difference in CLas titers. These findings suggest that ISVs can modulate the transmission dynamics of plant pathogens by insect vectors, adding complexity to vector-pathogen interactions.
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spelling doaj-art-c79f079ea875437b87cbd4488406b3ad2025-08-20T03:29:10ZengElsevieriScience2589-00422025-08-0128811298210.1016/j.isci.2025.112982Biological properties and vector competence of Diaphorina citri for Candidatus Liberibacter asiaticus modulated by an insect-specific virusDiogo M. Galdeano0Tanvi Rawat1William Ingram2Curtis R. Carlson3Gustavo Rodrigues Alves4Yen-Wen Kuo5Department of Plant Pathology, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA; Departamento de Fitopatologia, Universidade Federal de Viçosa, Av. P.H. Rolfs, s/n, Viçosa, MG 36570-900, BrazilDepartment of Plant Pathology, University of California-Davis, One Shields Avenue, Davis, CA 95616, USADepartment of Plant Pathology, University of California-Davis, One Shields Avenue, Davis, CA 95616, USADepartment of Entomology and Nematology, University of California-Davis, One Shields Avenue, Davis, CA 95616, USADepartmento de Entomologia, Escola Superior de Agricultura Luiz de Queiroz (ESALQ), Piracicaba, SP 13418-900, BrazilDepartment of Plant Pathology, University of California-Davis, One Shields Avenue, Davis, CA 95616, USA; Corresponding authorSummary: Insect-specific viruses (ISVs) infect only insects and have been studied primarily in mosquitoes. Here, we extend the concept of ISVs to include those found in plant-feeding insect vectors, such as Diaphorina citri, the vector of Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus Huanglongbing. Using Diaphorina citri flavi-like virus (DcFLV) as a model, we examined its effects on host biology and CLas transmission. DcFLV infection delayed development and preoviposition and reduced feeding activity, whereas overall survival, oviposition, and sex ratio were unaffected. DcFLV-infected D. citri transmitted CLas with ∼20% greater efficiency than uninfected controls, despite no significant difference in CLas titers. These findings suggest that ISVs can modulate the transmission dynamics of plant pathogens by insect vectors, adding complexity to vector-pathogen interactions.http://www.sciencedirect.com/science/article/pii/S258900422501243XEntomologyVirologyPlant Biology
spellingShingle Diogo M. Galdeano
Tanvi Rawat
William Ingram
Curtis R. Carlson
Gustavo Rodrigues Alves
Yen-Wen Kuo
Biological properties and vector competence of Diaphorina citri for Candidatus Liberibacter asiaticus modulated by an insect-specific virus
iScience
Entomology
Virology
Plant Biology
title Biological properties and vector competence of Diaphorina citri for Candidatus Liberibacter asiaticus modulated by an insect-specific virus
title_full Biological properties and vector competence of Diaphorina citri for Candidatus Liberibacter asiaticus modulated by an insect-specific virus
title_fullStr Biological properties and vector competence of Diaphorina citri for Candidatus Liberibacter asiaticus modulated by an insect-specific virus
title_full_unstemmed Biological properties and vector competence of Diaphorina citri for Candidatus Liberibacter asiaticus modulated by an insect-specific virus
title_short Biological properties and vector competence of Diaphorina citri for Candidatus Liberibacter asiaticus modulated by an insect-specific virus
title_sort biological properties and vector competence of diaphorina citri for candidatus liberibacter asiaticus modulated by an insect specific virus
topic Entomology
Virology
Plant Biology
url http://www.sciencedirect.com/science/article/pii/S258900422501243X
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