Cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stress
Autophagy is a highly conserved process of intracellular degradation of damaged, oxidized or excess macromolecules and organelles in eukaryotic cells. Under optimal conditions, the baseline level of autophagy is low. However, the activity of autophagic processes greatly increases in response to stre...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kazan Federal University
2020-12-01
|
| Series: | Учёные записки Казанского университета: Серия Естественные науки |
| Subjects: | |
| Online Access: | https://kpfu.ru/uz-eng-ns-2020-4-3.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841564828874833920 |
|---|---|
| author | A.B. Mazina S.A. Dmitrieva A.G. Renkova J.N. Valitova V.R. Khabibrakhmanova F.V. Minibayeva |
| author_facet | A.B. Mazina S.A. Dmitrieva A.G. Renkova J.N. Valitova V.R. Khabibrakhmanova F.V. Minibayeva |
| author_sort | A.B. Mazina |
| collection | DOAJ |
| description | Autophagy is a highly conserved process of intracellular degradation of damaged, oxidized or excess macromolecules and organelles in eukaryotic cells. Under optimal conditions, the baseline level of autophagy is low. However, the activity of autophagic processes greatly increases in response to stress. In this paper, we analyzed the biochemical characteristics of autophagosomes that are formed in the wheat suspension culture cells in response to starvation and as a result of treatment with polyamine spermine. High activity of acid phosphatase and protease, the marker enzymes of lytic vesicles, and changes in the lipid composition were observed in the autophagosomes isolated from the sucrose-deficient cells. It was revealed that polyamine spermine can induce аutophagy, which was accompanied with the changes in the cellular redox status and the energy status of mitochondria. The spermine-induced autophagosomes were characterized by the high enzymatic activity of acid phosphatase and protease. The results obtained suggest the functional universality of the main biochemical markers of autophagosomes in plant cells. |
| format | Article |
| id | doaj-art-c79d2cc187be477684c77a0fed223ad2 |
| institution | Kabale University |
| issn | 2542-064X 2500-218X |
| language | English |
| publishDate | 2020-12-01 |
| publisher | Kazan Federal University |
| record_format | Article |
| series | Учёные записки Казанского университета: Серия Естественные науки |
| spelling | doaj-art-c79d2cc187be477684c77a0fed223ad22025-01-02T22:16:58ZengKazan Federal UniversityУчёные записки Казанского университета: Серия Естественные науки2542-064X2500-218X2020-12-01162454155610.26907/2542-064X.2020.4.541-556Cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stressA.B. Mazina0S.A. Dmitrieva1A.G. Renkova2J.N. Valitova3V.R. Khabibrakhmanova4F.V. Minibayeva5Kazan Federal University, Kazan, 420008 Russia; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420111 RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420111 RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420111 RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420111 RussiaKazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420111 Russia; Kazan National Research Technological University, Kazan, 420015 RussiaKazan Federal University, Kazan, 420008 Russia; Kazan Institute of Biochemistry and Biophysics, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, 420111 RussiaAutophagy is a highly conserved process of intracellular degradation of damaged, oxidized or excess macromolecules and organelles in eukaryotic cells. Under optimal conditions, the baseline level of autophagy is low. However, the activity of autophagic processes greatly increases in response to stress. In this paper, we analyzed the biochemical characteristics of autophagosomes that are formed in the wheat suspension culture cells in response to starvation and as a result of treatment with polyamine spermine. High activity of acid phosphatase and protease, the marker enzymes of lytic vesicles, and changes in the lipid composition were observed in the autophagosomes isolated from the sucrose-deficient cells. It was revealed that polyamine spermine can induce аutophagy, which was accompanied with the changes in the cellular redox status and the energy status of mitochondria. The spermine-induced autophagosomes were characterized by the high enzymatic activity of acid phosphatase and protease. The results obtained suggest the functional universality of the main biochemical markers of autophagosomes in plant cells.https://kpfu.ru/uz-eng-ns-2020-4-3.htmlsuspension culture of wheatautophagosomesstarvationspermineacid phosphataseprotease |
| spellingShingle | A.B. Mazina S.A. Dmitrieva A.G. Renkova J.N. Valitova V.R. Khabibrakhmanova F.V. Minibayeva Cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stress Учёные записки Казанского университета: Серия Естественные науки suspension culture of wheat autophagosomes starvation spermine acid phosphatase protease |
| title | Cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stress |
| title_full | Cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stress |
| title_fullStr | Cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stress |
| title_full_unstemmed | Cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stress |
| title_short | Cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stress |
| title_sort | cytological and biochemical markers of autophagy in wheat suspension culture cells under abiotic stress |
| topic | suspension culture of wheat autophagosomes starvation spermine acid phosphatase protease |
| url | https://kpfu.ru/uz-eng-ns-2020-4-3.html |
| work_keys_str_mv | AT abmazina cytologicalandbiochemicalmarkersofautophagyinwheatsuspensionculturecellsunderabioticstress AT sadmitrieva cytologicalandbiochemicalmarkersofautophagyinwheatsuspensionculturecellsunderabioticstress AT agrenkova cytologicalandbiochemicalmarkersofautophagyinwheatsuspensionculturecellsunderabioticstress AT jnvalitova cytologicalandbiochemicalmarkersofautophagyinwheatsuspensionculturecellsunderabioticstress AT vrkhabibrakhmanova cytologicalandbiochemicalmarkersofautophagyinwheatsuspensionculturecellsunderabioticstress AT fvminibayeva cytologicalandbiochemicalmarkersofautophagyinwheatsuspensionculturecellsunderabioticstress |