Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation
Serum and BALF (bronchoalveolar lavage fluid) IL-8 levels and serum levels were investigated in Tox ocara canis infected guinea-pigs and the role of IL-5 as a modulator of cytokine secretion was studied. Serum levels increased early in infected animals, exceeding control levels 4 h after infection,...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1998-01-01
|
| Series: | Mediators of Inflammation |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09629359891379 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849764002027012096 |
|---|---|
| author | L. H. Faccioli A. I. Medeiros A. Malheiro R. C. L. R. Pietro A. Januário B. B. Vargaftig |
| author_facet | L. H. Faccioli A. I. Medeiros A. Malheiro R. C. L. R. Pietro A. Januário B. B. Vargaftig |
| author_sort | L. H. Faccioli |
| collection | DOAJ |
| description | Serum and BALF (bronchoalveolar lavage fluid) IL-8 levels and serum levels were investigated in Tox ocara canis infected guinea-pigs and the role of IL-5 as a modulator of cytokine secretion was studied. Serum levels increased early in infected animals, exceeding control levels 4 h after infection, peaked between days 6 and 18, and continued to exceed control levels after 48 days of infection. Serum and BALF IL-8 levels showed the same profile as blood eosinophilia, increasing 6 days post-infection and peaking between days 18 and 24. Treatment of infected animals with anti-IL-5 Ab suppressed eosinophilia with a parallel increase in blood IL-8 levels, whereas no change was found in levels. To support our in vivo observation we carried out experiments in vitro using guinea-pig LPS-stimulated adherent peritoneal cells which release large amounts of IL-8 into the supernatants. When rIL-5 was added to LPS-stimulated cells, 65% inhibition of IL-8 release into the supernatants was observed. Pre-incubation of cells with anti-IL-5 Ab prevented the inhibition of IL-8 release into the supernatants induced by rIL-5. Our results demonstrate for the first time that TNF- α and IL-8 are released concomitant with or after IL-5 in the eosinophilic inflammation induced by T. canis . Moreover, in addition to showing that IL-5 is fundamental for the induction of blood eosinophilia, the present results suggest that this cytokine may play a new biological role by acting as modulator of IL-8 secretion. |
| format | Article |
| id | doaj-art-c79c2b6ef1f547859e55f0025bf2ecb2 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-c79c2b6ef1f547859e55f0025bf2ecb22025-08-20T03:05:15ZengWileyMediators of Inflammation0962-93511466-18611998-01-0171414710.1080/09629359891379Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammationL. H. Faccioli0A. I. Medeiros1A. Malheiro2R. C. L. R. Pietro3A. Januário4B. B. Vargaftig5Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, BrazilDepartment of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, BrazilDepartment of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, BrazilDepartment of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, BrazilDepartment of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, BrazilUnité de Pharmacologie Cellulaire, Unité Associée Institut Pasteur/INSERM n. 285, Paris, FranceSerum and BALF (bronchoalveolar lavage fluid) IL-8 levels and serum levels were investigated in Tox ocara canis infected guinea-pigs and the role of IL-5 as a modulator of cytokine secretion was studied. Serum levels increased early in infected animals, exceeding control levels 4 h after infection, peaked between days 6 and 18, and continued to exceed control levels after 48 days of infection. Serum and BALF IL-8 levels showed the same profile as blood eosinophilia, increasing 6 days post-infection and peaking between days 18 and 24. Treatment of infected animals with anti-IL-5 Ab suppressed eosinophilia with a parallel increase in blood IL-8 levels, whereas no change was found in levels. To support our in vivo observation we carried out experiments in vitro using guinea-pig LPS-stimulated adherent peritoneal cells which release large amounts of IL-8 into the supernatants. When rIL-5 was added to LPS-stimulated cells, 65% inhibition of IL-8 release into the supernatants was observed. Pre-incubation of cells with anti-IL-5 Ab prevented the inhibition of IL-8 release into the supernatants induced by rIL-5. Our results demonstrate for the first time that TNF- α and IL-8 are released concomitant with or after IL-5 in the eosinophilic inflammation induced by T. canis . Moreover, in addition to showing that IL-5 is fundamental for the induction of blood eosinophilia, the present results suggest that this cytokine may play a new biological role by acting as modulator of IL-8 secretion.http://dx.doi.org/10.1080/09629359891379InterleukinsEosinophilsInflammationToxocara canis . |
| spellingShingle | L. H. Faccioli A. I. Medeiros A. Malheiro R. C. L. R. Pietro A. Januário B. B. Vargaftig Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation Mediators of Inflammation Interleukins Eosinophils Inflammation Toxocara canis . |
| title | Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation |
| title_full | Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation |
| title_fullStr | Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation |
| title_full_unstemmed | Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation |
| title_short | Interleukin-5 modulates interleukin-8 secretion in eosinophilic inflammation |
| title_sort | interleukin 5 modulates interleukin 8 secretion in eosinophilic inflammation |
| topic | Interleukins Eosinophils Inflammation Toxocara canis . |
| url | http://dx.doi.org/10.1080/09629359891379 |
| work_keys_str_mv | AT lhfaccioli interleukin5modulatesinterleukin8secretionineosinophilicinflammation AT aimedeiros interleukin5modulatesinterleukin8secretionineosinophilicinflammation AT amalheiro interleukin5modulatesinterleukin8secretionineosinophilicinflammation AT rclrpietro interleukin5modulatesinterleukin8secretionineosinophilicinflammation AT ajanuario interleukin5modulatesinterleukin8secretionineosinophilicinflammation AT bbvargaftig interleukin5modulatesinterleukin8secretionineosinophilicinflammation |