Lysosome-related proteins may have changes in the urinary exosomes of patients with acute gout attack
Abstract Background The autophagy–lysosome is intricately linked to the development of gout. At present, the diagnosis and monitoring of gout are mainly invasive tests, which cannot predict the occurrence of gout in the acute phase, and bring new pain to patients. This study focuses on the changes o...
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BMC
2025-01-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02272-5 |
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| author | Jitu Wang Na Liu Mei Hu Man Zhang |
| author_facet | Jitu Wang Na Liu Mei Hu Man Zhang |
| author_sort | Jitu Wang |
| collection | DOAJ |
| description | Abstract Background The autophagy–lysosome is intricately linked to the development of gout. At present, the diagnosis and monitoring of gout are mainly invasive tests, which cannot predict the occurrence of gout in the acute phase, and bring new pain to patients. This study focuses on the changes of lysosome-related proteins in urinary exosomes of patients with acute gout attack to explore the potential noninvasive biomarkers clinical application value. Methods Urine samples were collected from the subject and exosomes were extracted. To explore the differentially expressed proteins in urinary exosomes among acute gout patients (AD group), intermittent gout patients (ID group) and normal controls (NC group) by DIA mass spectrometry. Urinary exosomal lysosome associated proteins were analyzed and receiver operating characteristic (ROC) curves of differentially expressed proteins were drawn to evaluate their clinical value in monitoring acute gout attack. Results A total of 1896 proteins were detected between AD group and ID group, of which 121 proteins were differentially expressed (FC > 1.5 and p < 0.05). There were three lysosomal-related proteins differentially expressed in urinary exosomes between AD group and ID group. Compared with the ID group, the expression of Cathepsin Z (CTSZ) and AP-1 complex subunit beta-1 (AP1B1) was increased, while the expression of Lysosome-associated membrane glycoprotein 2 (LAMP2) was decreased in AD group. The ROC analysis showed that CTSZ, AP1B1 and LAMP2 had a strong ability to predict acute gout attack, with AUC of 0.826, 0.847 and 0.882, respectively. Conclusions There are many specific protein changes in the urinary exosomes of patients with acute gout attack. The urinary exosomes of patients with acute gout attack may exhibit alterations in lysosome-related proteins, particularly CTSZ, AP1B1, and LAMP2, which may become potential biomarkers for monitoring acute gout attack. |
| format | Article |
| id | doaj-art-c790af9c29124f4b8281a7f6a6bc8b89 |
| institution | Kabale University |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj-art-c790af9c29124f4b8281a7f6a6bc8b892025-01-26T12:21:47ZengBMCEuropean Journal of Medical Research2047-783X2025-01-013011810.1186/s40001-025-02272-5Lysosome-related proteins may have changes in the urinary exosomes of patients with acute gout attackJitu Wang0Na Liu1Mei Hu2Man Zhang3Clinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical UniversityClinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical UniversityClinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical UniversityClinical Laboratory Medicine, Beijing Shijitan Hospital, Capital Medical UniversityAbstract Background The autophagy–lysosome is intricately linked to the development of gout. At present, the diagnosis and monitoring of gout are mainly invasive tests, which cannot predict the occurrence of gout in the acute phase, and bring new pain to patients. This study focuses on the changes of lysosome-related proteins in urinary exosomes of patients with acute gout attack to explore the potential noninvasive biomarkers clinical application value. Methods Urine samples were collected from the subject and exosomes were extracted. To explore the differentially expressed proteins in urinary exosomes among acute gout patients (AD group), intermittent gout patients (ID group) and normal controls (NC group) by DIA mass spectrometry. Urinary exosomal lysosome associated proteins were analyzed and receiver operating characteristic (ROC) curves of differentially expressed proteins were drawn to evaluate their clinical value in monitoring acute gout attack. Results A total of 1896 proteins were detected between AD group and ID group, of which 121 proteins were differentially expressed (FC > 1.5 and p < 0.05). There were three lysosomal-related proteins differentially expressed in urinary exosomes between AD group and ID group. Compared with the ID group, the expression of Cathepsin Z (CTSZ) and AP-1 complex subunit beta-1 (AP1B1) was increased, while the expression of Lysosome-associated membrane glycoprotein 2 (LAMP2) was decreased in AD group. The ROC analysis showed that CTSZ, AP1B1 and LAMP2 had a strong ability to predict acute gout attack, with AUC of 0.826, 0.847 and 0.882, respectively. Conclusions There are many specific protein changes in the urinary exosomes of patients with acute gout attack. The urinary exosomes of patients with acute gout attack may exhibit alterations in lysosome-related proteins, particularly CTSZ, AP1B1, and LAMP2, which may become potential biomarkers for monitoring acute gout attack.https://doi.org/10.1186/s40001-025-02272-5Acute gout attackUrine exosomesLysosomeAutophagyBiomarker |
| spellingShingle | Jitu Wang Na Liu Mei Hu Man Zhang Lysosome-related proteins may have changes in the urinary exosomes of patients with acute gout attack European Journal of Medical Research Acute gout attack Urine exosomes Lysosome Autophagy Biomarker |
| title | Lysosome-related proteins may have changes in the urinary exosomes of patients with acute gout attack |
| title_full | Lysosome-related proteins may have changes in the urinary exosomes of patients with acute gout attack |
| title_fullStr | Lysosome-related proteins may have changes in the urinary exosomes of patients with acute gout attack |
| title_full_unstemmed | Lysosome-related proteins may have changes in the urinary exosomes of patients with acute gout attack |
| title_short | Lysosome-related proteins may have changes in the urinary exosomes of patients with acute gout attack |
| title_sort | lysosome related proteins may have changes in the urinary exosomes of patients with acute gout attack |
| topic | Acute gout attack Urine exosomes Lysosome Autophagy Biomarker |
| url | https://doi.org/10.1186/s40001-025-02272-5 |
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