A New Method for Preparation of Decellularized Human Scaffolds for Facial Reconstruction
Vascularized composite allotransplantation (VCA) has emerged as a robust alternative for addressing anatomically complex defects but requires a toxic lifelong immunosuppressive regimen. Tissue engineering offers the promise of creating recipient-specific alternative grafts using a decellularization...
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MDPI AG
2025-04-01
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| Series: | Current Issues in Molecular Biology |
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| author | Elise Lupon Aylin Acun Alec R. Andrews Ruben Oganesyan Hyshem H. Lancia Laurent Lantieri Mark A. Randolph Curtis L. Cetrulo Alexandre G. Lellouch Basak E. Uygun |
| author_facet | Elise Lupon Aylin Acun Alec R. Andrews Ruben Oganesyan Hyshem H. Lancia Laurent Lantieri Mark A. Randolph Curtis L. Cetrulo Alexandre G. Lellouch Basak E. Uygun |
| author_sort | Elise Lupon |
| collection | DOAJ |
| description | Vascularized composite allotransplantation (VCA) has emerged as a robust alternative for addressing anatomically complex defects but requires a toxic lifelong immunosuppressive regimen. Tissue engineering offers the promise of creating recipient-specific alternative grafts using a decellularization and recellularization approach. In this article, we establish a reliable protocol for human face decellularization by immersion as a new tool in the development of engineered graft alternatives for reconstructive surgery. Three cadaveric face grafts were immersed in 1% sodium dodecyl sulfate for 216 h followed by 1% Triton X-100 for 48 h, without perfusion through the pedicle. We determined that decellularization was successfully accomplished for three facial specimens as confirmed by histological evaluation and quantification of DNA content. The extracellular components including collagen, glycosaminoglycans, elastin, and matrix-bound growth factors were preserved. Vascular architecture did not show significant differences between native and decellularized grafts as imaged by X-ray angiography. The mechanical strength of the grafts was not altered after decellularization. We also showed that the decellularized grafts were biocompatible in vitro and in vivo allowing cell engraftment. As a result, we have successfully developed a protocol to yield a clinical size decellularized graft suitable for generating a recellularized, potentially non-immunogenic graft for facial reconstruction. |
| format | Article |
| id | doaj-art-c78b00c7fedb446aada78dd5a996f14b |
| institution | OA Journals |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-c78b00c7fedb446aada78dd5a996f14b2025-08-20T02:28:19ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452025-04-0147427510.3390/cimb47040275A New Method for Preparation of Decellularized Human Scaffolds for Facial ReconstructionElise Lupon0Aylin Acun1Alec R. Andrews2Ruben Oganesyan3Hyshem H. Lancia4Laurent Lantieri5Mark A. Randolph6Curtis L. Cetrulo7Alexandre G. Lellouch8Basak E. Uygun9Department of Plastic and Reconstructive Surgery, Institut Universitaire Locomoteur et du Sport, Pasteur 2 Hospital, University Côte d’Azur, 06107 Nice, FranceShriners Children’s Boston, Boston, MA 02114, USAVascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USAShriners Children’s Boston, Boston, MA 02114, USAVascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USAService de Chirurgie Plastique et Reconstructrice, Hôpital Européen Georges-Pompidou, 75015 Paris, FranceVascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USAVascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USAVascularized Composite Allotransplantation Laboratory, Center for Transplantation Sciences, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02115, USAShriners Children’s Boston, Boston, MA 02114, USAVascularized composite allotransplantation (VCA) has emerged as a robust alternative for addressing anatomically complex defects but requires a toxic lifelong immunosuppressive regimen. Tissue engineering offers the promise of creating recipient-specific alternative grafts using a decellularization and recellularization approach. In this article, we establish a reliable protocol for human face decellularization by immersion as a new tool in the development of engineered graft alternatives for reconstructive surgery. Three cadaveric face grafts were immersed in 1% sodium dodecyl sulfate for 216 h followed by 1% Triton X-100 for 48 h, without perfusion through the pedicle. We determined that decellularization was successfully accomplished for three facial specimens as confirmed by histological evaluation and quantification of DNA content. The extracellular components including collagen, glycosaminoglycans, elastin, and matrix-bound growth factors were preserved. Vascular architecture did not show significant differences between native and decellularized grafts as imaged by X-ray angiography. The mechanical strength of the grafts was not altered after decellularization. We also showed that the decellularized grafts were biocompatible in vitro and in vivo allowing cell engraftment. As a result, we have successfully developed a protocol to yield a clinical size decellularized graft suitable for generating a recellularized, potentially non-immunogenic graft for facial reconstruction.https://www.mdpi.com/1467-3045/47/4/275decellularizationrecellularizationvascularized composite allotransplantationfacial reconstructionregenerative medicinescaffold |
| spellingShingle | Elise Lupon Aylin Acun Alec R. Andrews Ruben Oganesyan Hyshem H. Lancia Laurent Lantieri Mark A. Randolph Curtis L. Cetrulo Alexandre G. Lellouch Basak E. Uygun A New Method for Preparation of Decellularized Human Scaffolds for Facial Reconstruction Current Issues in Molecular Biology decellularization recellularization vascularized composite allotransplantation facial reconstruction regenerative medicine scaffold |
| title | A New Method for Preparation of Decellularized Human Scaffolds for Facial Reconstruction |
| title_full | A New Method for Preparation of Decellularized Human Scaffolds for Facial Reconstruction |
| title_fullStr | A New Method for Preparation of Decellularized Human Scaffolds for Facial Reconstruction |
| title_full_unstemmed | A New Method for Preparation of Decellularized Human Scaffolds for Facial Reconstruction |
| title_short | A New Method for Preparation of Decellularized Human Scaffolds for Facial Reconstruction |
| title_sort | new method for preparation of decellularized human scaffolds for facial reconstruction |
| topic | decellularization recellularization vascularized composite allotransplantation facial reconstruction regenerative medicine scaffold |
| url | https://www.mdpi.com/1467-3045/47/4/275 |
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